3-hydroxyanthranilate 3,4-dioxygenase activity

Definition

Target type: molecularfunction

Catalysis of the reaction: 3-hydroxyanthranilate + O2 = cis,cis-2-amino-3-(3-oxoprop-1-enyl)but-2-enedioate + H+. [EC:1.13.11.6, RHEA:17953]

3-Hydroxyanthranilate 3,4-dioxygenase (HAAO) catalyzes the oxidative cleavage of 3-hydroxyanthranilate to 2-amino-3-carboxymuconate semialdehyde, an essential step in the tryptophan catabolic pathway. This reaction involves the incorporation of molecular oxygen into the aromatic ring of 3-hydroxyanthranilate, leading to the formation of a cyclic peroxide intermediate. This intermediate then undergoes ring opening and decarboxylation to yield the final product. The active site of HAAO contains a non-heme iron ion that plays a crucial role in the catalytic mechanism. The iron ion coordinates to a histidine residue and a water molecule, forming a mononuclear iron center. This iron center is responsible for activating molecular oxygen and facilitating the oxidation of 3-hydroxyanthranilate. The enzyme also requires a reducing agent, such as NADH, for its activity. In addition to its role in tryptophan catabolism, HAAO has also been implicated in other biological processes, including the biosynthesis of the neurotransmitter serotonin and the detoxification of xenobiotics. It has also been shown to play a role in the pathogenesis of certain diseases, such as cancer and neurodegenerative disorders.'
"

Proteins (1)

Compounds (1)