Page last updated: 2024-10-24

RPAP3/R2TP/prefoldin-like complex

Definition

Target type: cellularcomponent

A protein complex first characterized in human and comprised of a R2TP module (R2TP complex), a prefoldin-like module (containing both prefoldin-like proteins and canonical prefoldins), WD40 repeat protein Monad/WDR92 and DNA-dependent RNA polymerase subunit RPB5. This complex might have chaperone activity. [GOC:pr, PMID:20453924, PMID:21925213, PMID:22418846]

The RPAP3/R2TP/prefoldin-like complex is a multi-protein chaperone complex involved in the biogenesis of a diverse range of cellular components, including ribosomal subunits, RNA polymerase II, and other proteins. It plays a crucial role in the proper folding and assembly of these components, ensuring their functional integrity. The complex consists of multiple subunits:

* **RPAP3 (R2TP complex-associated protein 3)**: A key component of the complex, RPAP3 acts as a scaffold protein, mediating the interaction of other subunits and contributing to the complex's stability.
* **R2TP complex subunits (RUVBL1/RUVBL2, PIH1D1, and TAH1L)**: These subunits, together with RPAP3, form the core R2TP complex. RUVBL1/RUVBL2 are AAA+ ATPases that provide the energy for the complex's chaperone activity. PIH1D1 acts as a scaffold and facilitates the interaction of the complex with its substrates. TAH1L is a WD40 repeat protein that enhances the activity of R2TP.
* **Prefoldin-like subunits (PFDN1, PFDN2, PFDN3, PFDN4, PFDN5, and PFDN6)**: These subunits, also known as the prefoldin complex, contribute to the complex's ability to interact with and stabilize unfolded substrates.

The RPAP3/R2TP/prefoldin-like complex operates in the cytoplasm and the nucleus, where it interacts with its substrates, facilitating their folding and assembly. The complex has been implicated in a wide range of cellular processes, including ribosome biogenesis, RNA polymerase II transcription, protein trafficking, and DNA repair.
'
"

Proteins (2)

ProteinDefinitionTaxonomy
RuvB-like 1A RuvB-like 1 that is encoded in the genome of human. [PRO:DNx, UniProtKB:Q9Y265]Homo sapiens (human)
RuvB-like 2A RuvB-like 2 that is encoded in the genome of human. [PRO:DNx, UniProtKB:Q9Y230]Homo sapiens (human)

Compounds (3)

CompoundDefinitionClassesRoles
sorafenib(trifluoromethyl)benzenes;
aromatic ether;
monochlorobenzenes;
phenylureas;
pyridinecarboxamide
angiogenesis inhibitor;
anticoronaviral agent;
antineoplastic agent;
EC 2.7.11.1 (non-specific serine/threonine protein kinase) inhibitor;
ferroptosis inducer;
tyrosine kinase inhibitor
rottlerinrottlerin : A chromenol that is 2,2-dimethyl-2H-chromene substituted by hydroxy groups at positions 5 and 7, a 3-acetyl-2,4,6-trihydroxy-5-methylbenzyl group at position 6 and a (1E)-3-oxo-1-phenylprop-1-en-3-yl group at position 8. A potassium channel opener, it is isolated from Mallotus philippensis.

rottlerin: an angiogenesis inhibitor; an inhibitor of protein kinase Cdelta (PKCdelta) and calmodulin kinase III; RN refers to (E)-isomer; do not confuse this chalcone with an anthraquinone that is also called rottlerin (RN 481-72-1);
aromatic ketone;
benzenetriol;
chromenol;
enone;
methyl ketone
anti-allergic agent;
antihypertensive agent;
antineoplastic agent;
apoptosis inducer;
K-ATP channel agonist;
metabolite
akt-i-1,2 compoundAkt-I-1,2 compound: an aminopeptidase P inhibitor; structure in first source