CAF-1 complex
Definition
Target type: cellularcomponent
A conserved heterotrimeric protein complex that promotes histone H3 and H4 deposition onto newly synthesized DNA during replication or DNA repair; specifically facilitates replication-dependent nucleosome assembly with the major histone H3 (H3.1). In many species the CAF-1 subunits are designated p150, p60, and p48. [PMID:17065558, PMID:17083276]
The CAF-1 complex, also known as Chromatin Assembly Factor 1, is a multi-subunit protein complex that plays a crucial role in the assembly and replication of chromatin. The complex is composed of three subunits:
- **CAF-1A (p150)**: This subunit binds to both histone H3 and H4, and it has a histone chaperone function, meaning it can interact with and deliver histones to the DNA. It also contains a bromodomain, which recognizes acetylated lysines on histones, allowing it to associate with actively transcribed regions.
- **CAF-1B (p60)**: This subunit has a DNA-binding domain and is responsible for targeting the complex to replication forks. It interacts with the PCNA (proliferating cell nuclear antigen), a ring-shaped protein that encircles DNA during replication.
- **CAF-1C (p48)**: This subunit is involved in regulating the assembly of nucleosomes. It has a histone acetyltransferase (HAT) activity, meaning it can add acetyl groups to histones, which can modify their properties and influence gene expression.
The CAF-1 complex is recruited to replication forks by its interaction with PCNA. Once at the fork, the complex utilizes its histone chaperone function to deliver newly synthesized histones to the newly replicated DNA, ensuring the proper formation of nucleosomes. This process is essential for maintaining the integrity and stability of chromatin during DNA replication. The complex also plays a role in DNA repair, contributing to the accurate restoration of chromatin structure after damage.'
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Proteins (1)
| Protein | Definition | Taxonomy |
|---|---|---|
| Histone-binding protein RBBP4 | A histone-binding protein RBBP4 that is encoded in the genome of human. [PRO:DNx, UniProtKB:Q09028] | Homo sapiens (human) |
Compounds (3)
| Compound | Definition | Classes | Roles |
|---|---|---|---|
| przewaquinone d | przewaquinone D: isolated from root of Salvia przewalskii; structure given in first source; RN given refers to the trans- isomer, przewaquinone D | ||
| epz-6438 | tazemetostat: a histone methyltransferase EZH2 inhibitor with antineoplastic activity | ||
| gsk343 | GSK343 : A member of the class of indazoles that is 1-isopropyl-1H-indazole-4-carboxamide in which the nitrogen of the carboxamide group is substituted by a (6-methyl-2-oxo-4-propyl-1,2-dihydropyridin-3-yl)methyl group and in which the indazole ring is substituted at position 6 by a 2-(4-methylpiperazin-1-yl)pyridin-4-yl group. A highly potent and selective EZH2 inhibitor (IC50 = 4 nM). GSK343: an EZH2 methyltransferase inhibitor | aminopyridine; indazoles; N-alkylpiperazine; N-arylpiperazine; pyridone; secondary carboxamide | antineoplastic agent; apoptosis inducer; EC 2.1.1.43 (enhancer of zeste homolog 2) inhibitor |