E3 ubiquitin-protein ligase UHRF1

An E3 ubiquitin-protein ligase UHRF1 that is encoded in the genome of human. [PRO:DNx, UniProtKB:Q96T88]

Synonyms

EC 2.3.2.27;
Inverted CCAAT box-binding protein of 90 kDa;
Nuclear protein 95;
Nuclear zinc finger protein Np95;
HuNp95;
hNp95;
RING finger protein 106;
RING-type E3 ubiquitin transferase UHRF1;
Transcription factor ICBP90

Research

Bioassay Publications (2)

Timeframe	Studies on this Protein(%)	All Drugs %
pre-1990	0 (0.00)	18.7374
1990's	0 (0.00)	18.2507
2000's	0 (0.00)	29.6817
2010's	2 (100.00)	24.3611
2020's	0 (0.00)	2.80

Compounds (2)

Drugs with Inhibition Measurements

Drug	Taxonomy	Measurement	Average (mM)	Bioassay(s)	Publication(s)
(5-bromo-3-pyridinyl)-[4-(1-pyrrolidinyl)-1-piperidinyl]methanone	Homo sapiens (human)	IC50	20.0000	2	2
entecavir	Homo sapiens (human)	IC50	20.0000	2	2

Enables

This protein enables 14 target(s):

Target	Category	Definition
cis-regulatory region sequence-specific DNA binding	molecular function	Binding to a specific upstream regulatory DNA sequence (transcription factor recognition sequence or binding site, located in cis relative to the transcription start site (i.e., on the same strand of DNA) of a gene transcribed by some RNA polymerase. Cis-regulatory sites are often referred to as a sequence motifs, enhancers, or silencers. [GOC:txnOH-2018]
nucleic acid binding	molecular function	Binding to a nucleic acid. [GOC:jl]
ubiquitin-protein transferase activity	molecular function	Catalysis of the transfer of ubiquitin from one protein to another via the reaction X-Ub + Y = Y-Ub + X, where both X-Ub and Y-Ub are covalent linkages. [GOC:BioGRID, GOC:jh2, PMID:9635407]
protein binding	molecular function	Binding to a protein. [GOC:go_curators]
zinc ion binding	molecular function	Binding to a zinc ion (Zn). [GOC:ai]
methyl-CpG binding	molecular function	Binding to a methylated cytosine/guanine dinucleotide. [GOC:jl, PMID:11746232]
methylated histone binding	molecular function	Binding to a histone in which a residue has been modified by methylation. [GOC:bf, PMID:14585615]
histone binding	molecular function	Binding to a histone, any of a group of water-soluble proteins found in association with the DNA of eukaryotic or archaeal chromosomes. They are involved in the condensation and coiling of chromosomes during cell division and have also been implicated in gene regulation and DNA replication. They may be chemically modified (methylated, acetlyated and others) to regulate gene transcription. [GOC:jl, PMID:16209651, PMID:30212449, PMID:9305837]
identical protein binding	molecular function	Binding to an identical protein or proteins. [GOC:jl]
hemi-methylated DNA-binding	molecular function	Binding to double-stranded hemi-methylated DNA at replication foci (one strand methylated, while the other strand is unmethylated). Methylation of cytosine or adenine in DNA is an important mechanism for establishing stable heritable epigenetic marks. [GOC:imk, GOC:sp, PMID:18772889]
ubiquitin protein ligase activity	molecular function	Catalysis of the transfer of ubiquitin to a substrate protein via the reaction X-ubiquitin + S = X + S-ubiquitin, where X is either an E2 or E3 enzyme, the X-ubiquitin linkage is a thioester bond, and the S-ubiquitin linkage is an amide bond: an isopeptide bond between the C-terminal glycine of ubiquitin and the epsilon-amino group of lysine residues in the substrate or, in the linear extension of ubiquitin chains, a peptide bond the between the C-terminal glycine and N-terminal methionine of ubiquitin residues. [GOC:BioGRID, GOC:dph, GOC:mah, GOC:tb, PMID:22863777]
H3K9me3 modified histone binding	molecular function	Binding to a histone H3 in which the lysine residue at position 9 has been modified by trimethylation. [PMID:30110338]
DNA damage sensor activity	molecular function	A molecule that recognises toxic DNA structures, for example, double-strand breaks or collapsed replication forks, and initiates a signaling response. [PMID:31995034]
histone H3 ubiquitin ligase activity	molecular function	Catalysis of the transfer of ubiquitin to a histone H3 substrate. [PMID:25303536]

Located In

This protein is located in 4 target(s):

Target	Category	Definition
nucleus	cellular component	A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent. [GOC:go_curators]
nucleoplasm	cellular component	That part of the nuclear content other than the chromosomes or the nucleolus. [GOC:ma, ISBN:0124325653]
replication fork	cellular component	The Y-shaped region of a replicating DNA molecule, resulting from the separation of the DNA strands and in which the synthesis of new strands takes place. Also includes associated protein complexes. [GOC:mah, ISBN:0198547684]
nuclear matrix	cellular component	The dense fibrillar network lying on the inner side of the nuclear membrane. [ISBN:0582227089]

Active In

This protein is active in 1 target(s):

Target	Category	Definition
spindle	cellular component	The array of microtubules and associated molecules that forms between opposite poles of a eukaryotic cell during mitosis or meiosis and serves to move the duplicated chromosomes apart. [ISBN:0198547684]

Part Of

This protein is part of 3 target(s):

Target	Category	Definition
chromatin	cellular component	The ordered and organized complex of DNA, protein, and sometimes RNA, that forms the chromosome. [GOC:elh, PMID:20404130]
euchromatin	cellular component	A dispersed and relatively uncompacted form of chromatin that is in a transcription-competent conformation. [PMID:32017156]
heterochromatin	cellular component	A compact and highly condensed form of chromatin that is refractory to transcription. [PMID:32017156]

Involved In

This protein is involved in 15 target(s):

Target	Category	Definition
negative regulation of transcription by RNA polymerase II	biological process	Any process that stops, prevents, or reduces the frequency, rate or extent of transcription mediated by RNA polymerase II. [GOC:go_curators, GOC:txnOH]
double-strand break repair via homologous recombination	biological process	The error-free repair of a double-strand break in DNA in which the broken DNA molecule is repaired using homologous sequences. A strand in the broken DNA searches for a homologous region in an intact chromosome to serve as the template for DNA synthesis. The restoration of two intact DNA molecules results in the exchange, reciprocal or nonreciprocal, of genetic material between the intact DNA molecule and the broken DNA molecule. [GOC:elh, PMID:10357855]
ubiquitin-dependent protein catabolic process	biological process	The chemical reactions and pathways resulting in the breakdown of a protein or peptide by hydrolysis of its peptide bonds, initiated by the covalent attachment of a ubiquitin group, or multiple ubiquitin groups, to the protein. [GOC:go_curators]
DNA damage response	biological process	Any process that results in a change in state or activity of a cell (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a stimulus indicating damage to its DNA from environmental insults or errors during metabolism. [GOC:go_curators]
heterochromatin formation	biological process	An epigenetic gene silencing mechanism in which chromatin is compacted into heterochromatin, resulting in a chromatin conformation refractory to transcription. This process starts with heterochromatin nucleation, its spreading, and ends with heterochromatin boundary formation. [PMID:25192661, PMID:33827924]
homologous recombination	biological process	A DNA recombination process that results in the exchange of an equal amount of genetic material between highly homologous DNA molecules. [GOC:mah, PMID:11139492, PMID:17304215]
epigenetic regulation of gene expression	biological process	A process that modulates the frequency, rate or extent of gene expression through chromatin remodeling either by modifying higher order chromatin fiber structure, nucleosomal histones, or cytosine methylation of DNA. Once established, this regulation may be maintained over many cell divisions. It can also be heritable in the absence of the instigating signal. [PMID:10521337, PMID:11498582, PMID:22243696, PMID:34414474]
negative regulation of gene expression via chromosomal CpG island methylation	biological process	An epigenetic gene regulation mechanism that negatively regulates gene expression by methylation of cytosine residues in chromosomal CpG islands. CpG islands are genomic regions that contain a high frequency of the CG dinucleotide associated with the transcription start site of genes. [PMID:11898023, Wikipedia:Cpg_island]
positive regulation of transcription by RNA polymerase II	biological process	Any process that activates or increases the frequency, rate or extent of transcription from an RNA polymerase II promoter. [GOC:go_curators, GOC:txnOH]
regulation of epithelial cell proliferation	biological process	Any process that modulates the frequency, rate or extent of epithelial cell proliferation. [GOC:ai]
positive regulation of protein metabolic process	biological process	Any process that activates or increases the frequency, rate or extent of the chemical reactions and pathways involving a protein. [GOC:ai]
protein autoubiquitination	biological process	The ubiquitination by a protein of one or more of its own amino acid residues, or residues on an identical protein. Ubiquitination occurs on the lysine residue by formation of an isopeptide crosslink. [GOC:ai]
mitotic spindle assembly	biological process	Mitotic bipolar spindle assembly begins with spindle microtubule nucleation from the separated spindle pole body, includes spindle elongation during prometaphase, and is complete when all kinetochores are stably attached the spindle, and the spindle assembly checkpoint is satisfied. [GOC:tb, GOC:vw]
positive regulation of DNA topoisomerase (ATP-hydrolyzing) activity	biological process	Any process that activates or increases the frequency, rate or extent of DNA topoisomerase (ATP-hydrolyzing) activity. [GOC:mah]
protein ubiquitination	biological process	The process in which one or more ubiquitin groups are added to a protein. [GOC:ai]