Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase

A peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase that is encoded in the genome of human. [PRO:DNx, UniProtKB:Q96IV0]

PNGase;
hPNGase;
EC 3.5.1.52;
N-glycanase 1;
Peptide:N-glycanase

Research

Drug	Taxonomy	Measurement	Average (mM)	Bioassay(s)	Publication(s)
batyl alcohol	Homo sapiens (human)	Kd	221.5000	1	1
castanospermine	Homo sapiens (human)	Kd	94.3000	1	1
dactinomycin	Homo sapiens (human)	Kd	88.5000	1	1

This protein enables 3 target(s):

Target	Category	Definition
peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase activity	molecular function	Catalysis of the reaction: 4-N-(N-acetyl-D-glucosaminyl)-protein + H2O = N-acetyl-beta-D-glucosaminylamine + peptide L-aspartate. This reaction is the hydrolysis of an N4-(acetyl-beta-D-glucosaminyl)asparagine residue in which the N-acetyl-D-glucosamine residue may be further glycosylated, to yield a (substituted) N-acetyl-beta-D-glucosaminylamine and the peptide containing an aspartic residue. [EC:3.5.1.52]
protein binding	molecular function	Binding to a protein. [GOC:go_curators]
metal ion binding	molecular function	Binding to a metal ion. [GOC:ai]

This protein is located in 2 target(s):

Target	Category	Definition
cytoplasm	cellular component	The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures. [ISBN:0198547684]
cytosol	cellular component	The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes. [GOC:hjd, GOC:jl]

This protein is active in 2 target(s):

Target	Category	Definition
cytosol	cellular component	The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes. [GOC:hjd, GOC:jl]
nucleus	cellular component	A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent. [GOC:go_curators]

This protein is involved in 3 target(s):

Target	Category	Definition
protein folding	biological process	The process of assisting in the covalent and noncovalent assembly of single chain polypeptides or multisubunit complexes into the correct tertiary structure. [GOC:go_curators, GOC:rb]
glycoprotein catabolic process	biological process	The chemical reactions and pathways resulting in the breakdown of a glycoprotein, a protein that contains covalently bound glycose (i.e. monosaccharide) residues; the glycose occurs most commonly as oligosaccharide or fairly small polysaccharide but occasionally as monosaccharide. [GOC:go_curators, ISBN:0198506732]
protein deglycosylation	biological process	The removal of sugar residues from a glycosylated protein. [GOC:mah]