Page last updated: 2024-06-17 14:52:35

DNA replication ATP-dependent helicase/nuclease DNA2

Compounds

Enables

This protein enables 14 target(s):

TargetCategoryDefinition
DNA bindingmolecular functionAny molecular function by which a gene product interacts selectively and non-covalently with DNA (deoxyribonucleic acid). [GOC:dph, GOC:jl, GOC:tb, GOC:vw]
DNA helicase activitymolecular functionUnwinding of a DNA helix, driven by ATP hydrolysis. [GOC:jl]
helicase activitymolecular functionCatalysis of the reaction: ATP + H2O = ADP + phosphate, to drive the unwinding of a DNA or RNA helix. [GOC:jl]
nuclease activitymolecular functionCatalysis of the hydrolysis of ester linkages within nucleic acids. [ISBN:0198547684]
protein bindingmolecular functionBinding to a protein. [GOC:go_curators]
ATP bindingmolecular functionBinding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator. [ISBN:0198506732]
ATP hydrolysis activitymolecular functionCatalysis of the reaction: ATP + H2O = ADP + H+ phosphate. ATP hydrolysis is used in some reactions as an energy source, for example to catalyze a reaction or drive transport against a concentration gradient. [RHEA:13065]
site-specific endodeoxyribonuclease activity, specific for altered basemolecular functionCatalysis of the hydrolysis of ester linkages at specific sites within a deoxyribonucleic acid molecule by creating internal breaks. [GOC:jl]
5'-flap endonuclease activitymolecular functionCatalysis of the cleavage of a 5' flap structure in DNA, but not other DNA structures; processes the 5' ends of Okazaki fragments in lagging strand DNA synthesis. [PMID:9778254]
single-stranded DNA helicase activitymolecular functionCatalysis of the reaction: ATP + H2O = ADP + phosphate, in the presence of single-stranded DNA; drives the unwinding of a DNA helix. [GOC:jl]
5'-3' DNA helicase activitymolecular functionUnwinding a DNA helix in the 5' to 3' direction, driven by ATP hydrolysis. [EC:5.6.2.3, GOC:jl]
metal ion bindingmolecular functionBinding to a metal ion. [GOC:ai]
4 iron, 4 sulfur cluster bindingmolecular functionBinding to a 4 iron, 4 sulfur (4Fe-4S) cluster; this cluster consists of four iron atoms, with the inorganic sulfur atoms found between the irons and acting as bridging ligands. [GOC:ai, PMID:15952888, Wikipedia:Iron-sulfur_cluster]
RNA bindingmolecular functionBinding to an RNA molecule or a portion thereof. [GOC:jl, GOC:mah]

Located In

This protein is located in 5 target(s):

TargetCategoryDefinition
chromosome, telomeric regioncellular componentThe end of a linear chromosome, required for the integrity and maintenance of the end. A chromosome telomere usually includes a region of telomerase-encoded repeats the length of which rarely exceeds 20 bp each and that permits the formation of a telomeric loop (T-loop). The telomeric repeat region is usually preceded by a sub-telomeric region that is gene-poor but rich in repetitive elements. Some telomeres only consist of the latter part (for eg. D. melanogaster telomeres). [GOC:elh]
nucleuscellular componentA membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent. [GOC:go_curators]
nucleoplasmcellular componentThat part of the nuclear content other than the chromosomes or the nucleolus. [GOC:ma, ISBN:0124325653]
mitochondrioncellular componentA semiautonomous, self replicating organelle that occurs in varying numbers, shapes, and sizes in the cytoplasm of virtually all eukaryotic cells. It is notably the site of tissue respiration. [GOC:giardia, ISBN:0198506732]
mitochondrial nucleoidcellular componentThe region of a mitochondrion to which the DNA is confined. [GOC:jl]

Active In

This protein is active in 1 target(s):

TargetCategoryDefinition
cytoplasmcellular componentThe contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures. [ISBN:0198547684]

Part Of

This protein is part of 1 target(s):

TargetCategoryDefinition
gamma DNA polymerase complexcellular componentA DNA polymerase complex consisting of a large subunit, responsible for the catalytic activities, and a small accessory subunit. Functions in the replication and repair of mitochondrial DNA. [GOC:jl, PMID:12045093]

Involved In

This protein is involved in 17 target(s):

TargetCategoryDefinition
DNA replication checkpoint signalingbiological processA signal transduction process that contributes to a DNA replication checkpoint, that prevents the initiation of nuclear division until DNA replication is complete, thereby ensuring that progeny inherit a full complement of the genome. [GOC:curators, GOC:rn, PMID:11728327, PMID:12537518]
telomere maintenancebiological processAny process that contributes to the maintenance of proper telomeric length and structure by affecting and monitoring the activity of telomeric proteins, the length of telomeric DNA and the replication and repair of the DNA. These processes includes those that shorten, lengthen, replicate and repair the telomeric DNA sequences. [GOC:BHF, GOC:BHF_telomere, GOC:elh, GOC:rl, PMID:11092831]
DNA double-strand break processingbiological processThe 5' to 3' exonucleolytic resection of the DNA at the site of the break to form a 3' single-strand DNA overhang. [PMID:10357855]
DNA replicationbiological processThe cellular metabolic process in which a cell duplicates one or more molecules of DNA. DNA replication begins when specific sequences, known as origins of replication, are recognized and bound by the origin recognition complex, and ends when the original DNA molecule has been completely duplicated and the copies topologically separated. The unit of replication usually corresponds to the genome of the cell, an organelle, or a virus. The template for replication can either be an existing DNA molecule or RNA. [GOC:mah]
mitochondrial DNA replicationbiological processThe process in which new strands of DNA are synthesized in the mitochondrion. [GOC:ai]
base-excision repairbiological processIn base excision repair, an altered base is removed by a DNA glycosylase enzyme, followed by excision of the resulting sugar phosphate. The small gap left in the DNA helix is filled in by the sequential action of DNA polymerase and DNA ligase. [ISBN:0815316194]
telomere maintenance via semi-conservative replicationbiological processThe process in which telomeric DNA is synthesized semi-conservatively by the conventional replication machinery and telomeric accessory factors as part of cell cycle DNA replication. [GOC:BHF, GOC:BHF_telomere, GOC:rl, GOC:vw, PMID:16598261]
DNA duplex unwindingbiological processThe process in which interchain hydrogen bonds between two strands of DNA are broken or 'melted', generating a region of unpaired single strands. [GOC:isa_complete, GOC:mah]
DNA replication, Okazaki fragment processingbiological processThe DNA metabolic process, occurring during lagging strand synthesis, by which RNA primers are removed from Okazaki fragments, the resulting gaps filled by DNA polymerization, and the ends ligated to form a continuous strand. [GOC:mah, ISBN:0716720094]
DNA replication, removal of RNA primerbiological processRemoval of the Okazaki RNA primer from the lagging strand of replicating DNA, by a combination of the actions of DNA polymerase, DNA helicase and an endonuclease. [GOC:jl, PMID:12424238]
mitochondrial DNA repairbiological processThe process of restoring mitochondrial DNA after damage. [PMID:12565799, PMID:15189144, PMID:16050976]
G-quadruplex DNA unwindingbiological processThe process by which G-quadruplex (also known as G4) DNA, which is a four-stranded DNA structure held together by guanine base pairing, is unwound or 'melted'. [GOC:jl, GOC:se, PMID:23657261]
positive regulation of DNA replicationbiological processAny process that activates or increases the frequency, rate or extent of DNA replication. [GOC:go_curators]
nucleic acid metabolic processbiological processAny cellular metabolic process involving nucleic acids. [GOC:dph, GOC:tb]
t-circle formationbiological processA telomere maintenance process that results in the formation of a telomeric circle, or t-circle. A t-circle is an extrachromosomal duplex or single-stranded circular DNA molecule composed of t-arrays. T-circles are involved in the control of telomere length via alternative-lengthening of telomeres (ALT) pathway and telomere rapid deletion (TRD). [GOC:BHF, GOC:BHF_telomere, GOC:nc, PMID:19214183, PMID:19581589, PMID:19809492, PMID:19858100]
mitotic telomere maintenance via semi-conservative replicationbiological processAny telomere maintenance via semi-conservative replication that is involved in mitotic cell cycle. [GO_REF:0000060, GOC:TermGenie]
replication fork reversalbiological processReplication fork processing that involves the unwinding of blocked forks to form four-stranded structures resembling Holliday junctions, which are subsequently resolved. [PMID:19406929]