DNA-directed RNA polymerase II subunit RPB1

A DNA-directed RNA polymerase II subunit RPB1 that is encoded in the genome of human. [PRO:DNx, UniProtKB:P24928]

Synonyms

RNA polymerase II subunit B1;
EC 2.7.7.6;
DNA-directed RNA polymerase II subunit A;
DNA-directed RNA polymerase III largest subunit;
RNA-directed RNA polymerase II subunit RPB1;
2.7.7.48

Research

Bioassay Publications (1)

Timeframe	Studies on this Protein(%)	All Drugs %
pre-1990	0 (0.00)	18.7374
1990's	0 (0.00)	18.2507
2000's	0 (0.00)	29.6817
2010's	1 (100.00)	24.3611
2020's	0 (0.00)	2.80

Compounds (1)

Drugs with Inhibition Measurements

Drug	Taxonomy	Measurement	Average (mM)	Bioassay(s)	Publication(s)
3'-deoxycytidine 5'-triphosphate	Homo sapiens (human)	IC50	500.0000	1	1

Enables

This protein enables 12 target(s):

Target	Category	Definition
magnesium ion binding	molecular function	Binding to a magnesium (Mg) ion. [GOC:ai]
DNA binding	molecular function	Any molecular function by which a gene product interacts selectively and non-covalently with DNA (deoxyribonucleic acid). [GOC:dph, GOC:jl, GOC:tb, GOC:vw]
RNA binding	molecular function	Binding to an RNA molecule or a portion thereof. [GOC:jl, GOC:mah]
DNA-directed 5'-3' RNA polymerase activity	molecular function	Catalysis of the reaction: nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1). Utilizes a DNA template, i.e. the catalysis of DNA-template-directed extension of the 3'-end of an RNA strand by one nucleotide at a time. Can initiate a chain 'de novo'. [EC:2.7.7.6, GOC:pf]
RNA-dependent RNA polymerase activity	molecular function	Catalysis of the reaction: nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1); uses an RNA template, i.e. the catalysis of RNA-template-directed extension of the 3'-end of an RNA strand by one nucleotide at a time. [EC:2.7.7.48, GOC:mah, GOC:pf]
protein binding	molecular function	Binding to a protein. [GOC:go_curators]
zinc ion binding	molecular function	Binding to a zinc ion (Zn). [GOC:ai]
hydrolase activity	molecular function	Catalysis of the hydrolysis of various bonds, e.g. C-O, C-N, C-C, phosphoric anhydride bonds, etc. [ISBN:0198506732]
kinase binding	molecular function	Binding to a kinase, any enzyme that catalyzes the transfer of a phosphate group. [GOC:jl]
ubiquitin protein ligase binding	molecular function	Binding to a ubiquitin protein ligase enzyme, any of the E3 proteins. [GOC:vp]
microfibril binding	molecular function	Binding to a microfibril, any small fibril occurring in biological material. [GOC:ai]
promoter-specific chromatin binding	molecular function	Binding to a section of chromatin that is associated with gene promoter sequences of DNA. [PMID:19948729]

Located In

This protein is located in 4 target(s):

Target	Category	Definition
nucleus	cellular component	A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent. [GOC:go_curators]
nucleoplasm	cellular component	That part of the nuclear content other than the chromosomes or the nucleolus. [GOC:ma, ISBN:0124325653]
chromosome	cellular component	A structure composed of a very long molecule of DNA and associated proteins (e.g. histones) that carries hereditary information. [ISBN:0198547684]
cytoplasm	cellular component	The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures. [ISBN:0198547684]

Part Of

This protein is part of 2 target(s):

Target	Category	Definition
Prp19 complex	cellular component	A protein complex consisting of Prp19 and associated proteins that is involved in the transition from the precatalytic spliceosome to the activated form that catalyzes step 1 of splicing, and which remains associated with the spliceosome through the second catalytic step. It is widely conserved, found in both yeast and mammals, though the exact composition varies. In S. cerevisiae, it contains Prp19p, Ntc20p, Snt309p, Isy1p, Syf2p, Cwc2p, Prp46p, Clf1p, Cef1p, and Syf1p. [GOC:krc, PMID:16540691, PMID:19239890]
RNA polymerase II, core complex	cellular component	RNA polymerase II, one of three nuclear DNA-directed RNA polymerases found in all eukaryotes, is a multisubunit complex; typically it produces mRNAs, snoRNAs, and some of the snRNAs. Two large subunits comprise the most conserved portion including the catalytic site and share similarity with other eukaryotic and bacterial multisubunit RNA polymerases. The largest subunit of RNA polymerase II contains an essential carboxyl-terminal domain (CTD) composed of a variable number of heptapeptide repeats (YSPTSPS). The remainder of the complex is composed of smaller subunits (generally ten or more), some of which are also found in RNA polymerases I and III. Although the core is competent to mediate ribonucleic acid synthesis, it requires additional factors to select the appropriate template. [GOC:krc, GOC:mtg_sensu]

Involved In

This protein is involved in 5 target(s):

Target	Category	Definition
RNA-templated transcription	biological process	The synthesis of an RNA transcript from an RNA template. [GOC:txnOH]
DNA-templated transcription termination	biological process	The completion of transcription: the RNA polymerase pauses, the RNA-DNA hybrid dissociates, followed by the release of the RNA polymerase from its DNA template. [GOC:txnOH, PMID:15020047, PMID:18280161, PMID:30978344]
regulation of DNA-templated transcription	biological process	Any process that modulates the frequency, rate or extent of cellular DNA-templated transcription. [GOC:go_curators, GOC:txnOH]
transcription by RNA polymerase II	biological process	The synthesis of RNA from a DNA template by RNA polymerase II (RNAP II), originating at an RNA polymerase II promoter. Includes transcription of messenger RNA (mRNA) and certain small nuclear RNAs (snRNAs). [GOC:jl, GOC:txnOH, ISBN:0321000382]
positive regulation of RNA splicing	biological process	Any process that activates or increases the frequency, rate or extent of RNA splicing. [GOC:mah]