Transcription initiation factor TFIID subunit 1

A transcription initiation factor TFIID subunit 1 that is encoded in the genome of human. [PRO:DNx]

Synonyms

EC 2.3.1.48;
EC 2.7.11.1;
Cell cycle gene 1 protein;
TBP-associated factor 250 kDa;
p250;
Transcription initiation factor TFIID 250 kDa subunit;
TAF(II)250;
TAFII-250;
TAFII250

Research

Bioassay Publications (3)

Timeframe	Studies on this Protein(%)	All Drugs %
pre-1990	0 (0.00)	18.7374
1990's	0 (0.00)	18.2507
2000's	0 (0.00)	29.6817
2010's	2 (66.67)	24.3611
2020's	1 (33.33)	2.80

Compounds (3)

Drugs with Inhibition Measurements

Drug	Taxonomy	Measurement	Average (mM)	Bioassay(s)	Publication(s)
bi 2536	Homo sapiens (human)	IC50	0.1600	1	1
i-bet726	Homo sapiens (human)	IC50	31.6228	1	1

Drugs with Activation Measurements

Drug	Taxonomy	Measurement	Average (mM)	Bioassay(s)	Publication(s)
bi 2536	Homo sapiens (human)	Kd	0.1700	1	1
(3R)-4-[2-(1H-indol-4-yl)-6-(1-methylsulfonylcyclopropyl)-4-pyrimidinyl]-3-methylmorpholine	Homo sapiens (human)	Kd	20.2918	4	4

Enables

This protein enables 20 target(s):

Target	Category	Definition
RNA polymerase II core promoter sequence-specific DNA binding	molecular function	Binding to a DNA sequence that is part of the core promoter of a RNA polymerase II-transcribed gene. [GOC:pg, GOC:txnOH, PMID:12381658]
RNA polymerase II general transcription initiation factor binding	molecular function	Binding to a basal RNA polymerase II transcription factor, any of the factors involved in formation of the preinitiation complex (PIC) by RNA polymerase II and defined as a basal or general transcription factor. [GOC:txnOH, PMID:16858867]
RNA polymerase I general transcription initiation factor activity	molecular function	A general transcription initiation factor activity that contributes to transcription start site selection and transcription initiation of genes transcribed by RNA polymerase I. Factors required for RNA polymerase I transcription initiation include upstream activation factor (UAF), core factor (CF), TATA binding protein (TBP) and RRN3. In all species characterized, RNA polymerase I transcribes a large polycistronic transcript that is processed into several mature rRNAs (3 or 4 depending on the species), including the large subunit rRNA (28S in humans), the small subunit rRNA (18S in humans), as well as one or two additional smaller rRNAs (the 5.8S rRNA in humans). In most species, this large rRNA transcript is the sole product of RNA polymerase I. However there are rare exceptions, such as Trypanosoma brucei, where RNA polymerase I also transcribes certain mRNAs. [GOC:txnOH-2018, PMID:11500378, PMID:17972917, PMID:25346433, PMID:28340337, PMID:28842442, PMID:31358304]
p53 binding	molecular function	Binding to one of the p53 family of proteins. [GOC:hjd]
histone acetyltransferase activity	molecular function	Catalysis of the reaction: acetyl-CoA + histone = CoA + acetyl-histone. [EC:2.3.1.48]
protein kinase activity	molecular function	Catalysis of the phosphorylation of an amino acid residue in a protein, usually according to the reaction: a protein + ATP = a phosphoprotein + ADP. [PMID:25399640]
protein serine/threonine kinase activity	molecular function	Catalysis of the reactions: ATP + protein serine = ADP + protein serine phosphate, and ATP + protein threonine = ADP + protein threonine phosphate. [GOC:bf, MetaCyc:PROTEIN-KINASE-RXN, PMID:2956925]
protein binding	molecular function	Binding to a protein. [GOC:go_curators]
ATP binding	molecular function	Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator. [ISBN:0198506732]
RNA polymerase II general transcription initiation factor activity	molecular function	A general transcription initiation factor activity that contributes to transcription start site selection and transcription initiation of genes transcribed by RNA polymerase II. The general transcription factors for RNA polymerase II include TFIIB, TFIID, TFIIE, TFIIF, TFIIH and TATA-binding protein (TBP). In most species, RNA polymerase II transcribes all messenger RNAs (mRNAs), most untranslated regulatory RNAs, the majority of the snoRNAs, four of the five snRNAs (U1, U2, U4, and U5), and other small noncoding RNAs. For some small RNAs there is variability between species as to whether it is transcribed by RNA polymerase II or RNA polymerase III. However there are also rare exceptions, such as Trypanosoma brucei, where RNA polymerase I transcribes certain mRNAs in addition to its normal role in rRNA transcription. [GOC:txnOH-2018, PMID:10384286, PMID:10747032, PMID:23442138, PMID:25693126]
kinase activity	molecular function	Catalysis of the transfer of a phosphate group, usually from ATP, to a substrate molecule. [ISBN:0198506732]
nuclear receptor binding	molecular function	Binding to a nuclear receptor protein. Nuclear receptor proteins are DNA-binding transcription factors which are regulated by binding to a ligand. [PMID:7776974]
TBP-class protein binding	molecular function	Binding to a member of the class of TATA-binding proteins (TBP), including any of the TBP-related factors (TRFs). [GOC:jl, GOC:txnOH, http://www.mblab.gla.ac.uk/, PMID:16858867]
sequence-specific DNA binding	molecular function	Binding to DNA of a specific nucleotide composition, e.g. GC-rich DNA binding, or with a specific sequence motif or type of DNA e.g. promotor binding or rDNA binding. [GOC:jl]
protein heterodimerization activity	molecular function	Binding to a nonidentical protein to form a heterodimer. [GOC:ai]
RNA polymerase II-specific DNA-binding transcription factor binding	molecular function	Binding to a sequence-specific DNA binding RNA polymerase II transcription factor, any of the factors that interact selectively and non-covalently with a specific DNA sequence in order to modulate transcription. [GOC:dph, GOC:vw]
ubiquitin conjugating enzyme activity	molecular function	Isoenergetic transfer of ubiquitin from one protein to another via the reaction X-ubiquitin + Y = Y-ubiquitin + X, where both the X-ubiquitin and Y-ubiquitin linkages are thioester bonds between the C-terminal glycine of ubiquitin and a sulfhydryl side group of a cysteine residue. [GOC:BioGRID, GOC:dph]
lysine-acetylated histone binding	molecular function	Binding to a histone in which a lysine residue has been modified by acetylation. [GOC:BHF, GOC:mah, GOC:rl, PMID:17582821]
protein serine kinase activity	molecular function	Catalysis of the reactions: ATP + protein serine = ADP + protein serine phosphate. [RHEA:17989]
transcription regulator inhibitor activity	molecular function	A molecular function regulator that inhibits the activity of a transcription regulator via direct binding and/or post-translational modification. [PMID:10652346]

Located In

This protein is located in 2 target(s):

Target	Category	Definition
nucleus	cellular component	A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent. [GOC:go_curators]
nucleoplasm	cellular component	That part of the nuclear content other than the chromosomes or the nucleolus. [GOC:ma, ISBN:0124325653]

Part Of

This protein is part of 4 target(s):

Target	Category	Definition
transcription factor TFIID complex	cellular component	A complex composed of TATA binding protein (TBP) and TBP associated factors (TAFs); the total mass is typically about 800 kDa. Most of the TAFs are conserved across species. In TATA-containing promoters for RNA polymerase II (Pol II), TFIID is believed to recognize at least two distinct elements, the TATA element and a downstream promoter element. TFIID is also involved in recognition of TATA-less Pol II promoters. Binding of TFIID to DNA is necessary but not sufficient for transcription initiation from most RNA polymerase II promoters. [GOC:krc, GOC:mah, ISBN:0471953393, ISBN:0879695501]
chromatin	cellular component	The ordered and organized complex of DNA, protein, and sometimes RNA, that forms the chromosome. [GOC:elh, PMID:20404130]
transcription regulator complex	cellular component	A protein complex that is capable of associating with DNA by direct binding, or via other DNA-binding proteins or complexes, and regulating transcription. [GOC:jl]
MLL1 complex	cellular component	A protein complex that can methylate lysine-4 of histone H3. MLL1/MLL is the catalytic methyltransferase subunit, and the complex also contains the core components ASH2L, HCFC1/HCF1 WDR5 and RBBP5. [GOC:sp, PMID:15960975]

Involved In

This protein is involved in 28 target(s):

Target	Category	Definition
negative regulation of transcription by RNA polymerase II	biological process	Any process that stops, prevents, or reduces the frequency, rate or extent of transcription mediated by RNA polymerase II. [GOC:go_curators, GOC:txnOH]
protein polyubiquitination	biological process	Addition of multiple ubiquitin groups to a protein, forming a ubiquitin chain. [ISBN:0815316194]
chromatin remodeling	biological process	A dynamic process of chromatin reorganization resulting in changes to chromatin structure. These changes allow DNA metabolic processes such as transcriptional regulation, DNA recombination, DNA repair, and DNA replication. [GOC:jid, GOC:vw, PMID:12042764, PMID:12697820]
transcription initiation at RNA polymerase I promoter	biological process	A transcription initiation process that takes place at a RNA polymerase I gene promoter. Ribosomal RNAs (rRNA) genes are transcribed by RNA polymerase I. [GOC:txnOH]
transcription by RNA polymerase II	biological process	The synthesis of RNA from a DNA template by RNA polymerase II (RNAP II), originating at an RNA polymerase II promoter. Includes transcription of messenger RNA (mRNA) and certain small nuclear RNAs (snRNAs). [GOC:jl, GOC:txnOH, ISBN:0321000382]
transcription initiation at RNA polymerase II promoter	biological process	A transcription initiation process that takes place at a RNA polymerase II gene promoter. Messenger RNAs (mRNA) genes, as well as some non-coding RNAs, are transcribed by RNA polymerase II. [GOC:mah, GOC:txnOH]
protein phosphorylation	biological process	The process of introducing a phosphate group on to a protein. [GOC:hb]
ubiquitin-dependent protein catabolic process	biological process	The chemical reactions and pathways resulting in the breakdown of a protein or peptide by hydrolysis of its peptide bonds, initiated by the covalent attachment of a ubiquitin group, or multiple ubiquitin groups, to the protein. [GOC:go_curators]
DNA damage response	biological process	Any process that results in a change in state or activity of a cell (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a stimulus indicating damage to its DNA from environmental insults or errors during metabolism. [GOC:go_curators]
negative regulation of gene expression	biological process	Any process that decreases the frequency, rate or extent of gene expression. Gene expression is the process in which a gene's coding sequence is converted into a mature gene product (protein or RNA). [GOC:txnOH-2018]
peptidyl-serine phosphorylation	biological process	The phosphorylation of peptidyl-serine to form peptidyl-O-phospho-L-serine. [RESID:AA0037]
peptidyl-threonine phosphorylation	biological process	The phosphorylation of peptidyl-threonine to form peptidyl-O-phospho-L-threonine. [RESID:AA0038]
midbrain development	biological process	The process whose specific outcome is the progression of the midbrain over time, from its formation to the mature structure. The midbrain is the middle division of the three primary divisions of the developing chordate brain or the corresponding part of the adult brain (in vertebrates, includes a ventral part containing the cerebral peduncles and a dorsal tectum containing the corpora quadrigemina and that surrounds the aqueduct of Sylvius connecting the third and fourth ventricles). [http://www2.merriam-webster.com/cgi-bin/mwmednlm?book=Medical&va=midbrain]
positive regulation of protein binding	biological process	Any process that activates or increases the frequency, rate or extent of protein binding. [GOC:mah]
positive regulation of proteasomal ubiquitin-dependent protein catabolic process	biological process	Any process that activates or increases the frequency, rate or extent of the breakdown of a protein or peptide by hydrolysis of its peptide bonds, initiated by the covalent attachment of ubiquitin, and mediated by the proteasome. [GOC:mah]
cellular response to UV	biological process	Any process that results in a change in state or activity of a cell (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of an ultraviolet radiation (UV light) stimulus. Ultraviolet radiation is electromagnetic radiation with a wavelength in the range of 10 to 380 nanometers. [GOC:mah]
mRNA transcription by RNA polymerase II	biological process	The cellular synthesis of messenger RNA (mRNA) from a DNA template by RNA polymerase II, originating at an RNA polymerase II promoter. [GOC:jl, ISBN:0321000382]
positive regulation of transcription by RNA polymerase II	biological process	Any process that activates or increases the frequency, rate or extent of transcription from an RNA polymerase II promoter. [GOC:go_curators, GOC:txnOH]
protein autophosphorylation	biological process	The phosphorylation by a protein of one or more of its own amino acid residues (cis-autophosphorylation), or residues on an identical protein (trans-autophosphorylation). [ISBN:0198506732]
protein stabilization	biological process	Any process involved in maintaining the structure and integrity of a protein and preventing it from degradation or aggregation. [GOC:ai]
RNA polymerase II preinitiation complex assembly	biological process	The formation of a large multiprotein-DNA complex that self-assembles on gene promoter through the sequential recruitment of the general initiation factors that compose the preinitiation complex (PIC) (which may include TFIIA, TFIIB, TFIID, TFIIE, TFIIF, and TFIIH complexes). The PIC engages RNA polymerase II on its DNA template strand and sparks polymerization of the first few RNA nucleotides of the nascent transcript, of which 8 are base-paired with the DNA template within a DNA bubble. PIC assembly may result in a pause step, which marks the end of the PIC assembly and may be followed by promoter clearance (exact synonym: promoter escape). For RNA polymerase II PIC assembly is preceded by the formation of a nucleosome-free region that allows the transcription machinery to access the promoter DNA. [GOC:txnOH, PMID:15020047, PMID:31300188]
positive regulation of transcription initiation by RNA polymerase II	biological process	Any process that increases the rate, frequency or extent of a process involved in starting transcription from an RNA polymerase II promoter. [GOC:dph, GOC:tb, GOC:txnOH]
cellular response to ATP	biological process	Any process that results in a change in state or activity of a cell (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of an ATP (adenosine 5'-triphosphate) stimulus. [GOC:mah]
regulation of signal transduction by p53 class mediator	biological process	Any process that modulates the frequency, rate or extent of signal transduction by p53 class mediator. [GOC:TermGenie]
regulation of cell cycle G1/S phase transition	biological process	Any signaling pathway that modulates the activity of a cell cycle cyclin-dependent protein kinase to modulate the switch from G1 phase to S phase of the cell cycle. [GO_REF:0000058, GOC:mtg_cell_cycle, GOC:TermGenie]
negative regulation of protein autoubiquitination	biological process	Any process that stops, prevents or reduces the frequency, rate or extent of protein autoubiquitination. [GO_REF:0000058, GOC:bc, GOC:PARL, GOC:TermGenie, PMID:17237821]
negative regulation of ubiquitin-dependent protein catabolic process	biological process	Any process that stops, prevents, or reduces the frequency, rate or extent of ubiquitin-dependent protein catabolic process. [GOC:BHF]
positive regulation of androgen receptor activity	biological process	Any process that activates or increases the frequency, rate or extent of androgen receptor activity. [GOC:obol]

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