Peptidyl-glycine alpha-amidating monooxygenase

A peptidyl-glycine alpha-amidating monooxygenase that is encoded in the genome of human. [PRO:DNx, UniProtKB:P19021]

PAM

Research

Drug	Taxonomy	Measurement	Average (mM)	Bioassay(s)	Publication(s)
aceturic acid	Homo sapiens (human)	Km	8,700.0000	2	2
prolylglycine	Homo sapiens (human)	Km	930.0000	2	2
phenylalanylglycine	Homo sapiens (human)	Km	6,250.0000	2	2

This protein enables 7 target(s):

Target	Category	Definition
peptidylglycine monooxygenase activity	molecular function	Catalysis of the reaction: peptidyl-glycine + ascorbate + O2 = peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O. [EC:1.14.17.3]
peptidylamidoglycolate lyase activity	molecular function	Catalysis of the reaction: peptidylamidoglycolate = peptidyl amide + glyoxylate. [EC:4.3.2.5]
copper ion binding	molecular function	Binding to a copper (Cu) ion. [GOC:ai]
calcium ion binding	molecular function	Binding to a calcium ion (Ca2+). [GOC:ai]
protein binding	molecular function	Binding to a protein. [GOC:go_curators]
zinc ion binding	molecular function	Binding to a zinc ion (Zn). [GOC:ai]
L-ascorbic acid binding	molecular function	Binding to L-ascorbic acid, (2R)-2-[(1S)-1,2-dihydroxyethyl]-4-hydroxy-5-oxo-2,5-dihydrofuran-3-olate; L-ascorbic acid is vitamin C and has co-factor and anti-oxidant activities in many species. [GOC:mah]

This protein is located in 4 target(s):

Target	Category	Definition
membrane	cellular component	A lipid bilayer along with all the proteins and protein complexes embedded in it and attached to it. [GOC:dos, GOC:mah, ISBN:0815316194]
transport vesicle membrane	cellular component	The lipid bilayer surrounding a transport vesicle. [GOC:mah]
secretory granule membrane	cellular component	The lipid bilayer surrounding a secretory granule. [GOC:mah]
extracellular exosome	cellular component	A vesicle that is released into the extracellular region by fusion of the limiting endosomal membrane of a multivesicular body with the plasma membrane. Extracellular exosomes, also simply called exosomes, have a diameter of about 40-100 nm. [GOC:BHF, GOC:mah, GOC:vesicles, PMID:15908444, PMID:17641064, PMID:19442504, PMID:19498381, PMID:22418571, PMID:24009894]

This protein is active in 1 target(s):

Target	Category	Definition
extracellular region	cellular component	The space external to the outermost structure of a cell. For cells without external protective or external encapsulating structures this refers to space outside of the plasma membrane. This term covers the host cell environment outside an intracellular parasite. [GOC:go_curators]

This protein is involved in 3 target(s):

Target	Category	Definition
peptide amidation	biological process	The posttranslational conversion of C-terminal glycine-extended peptides to C-terminal alpha-amidated peptides. Occurs to over half of all peptide hormones to give bioactive peptides. This is a two step process catalyzed by a peptidyl-glycine alpha-hydroxylating monooxygenase and a peptidyl-alpha-hydroxyglycine alpha-amidating lyase. In some organisms, this process is catalyzed by two separate enzymes, whereas in higher organisms, one polypeptide catalyzes both reactions. [PMID:11028916]
response to zinc ion	biological process	Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a zinc ion stimulus. [GOC:sm]
fatty acid primary amide biosynthetic process	biological process	The chemical reactions and pathways resulting in the formation of a fatty acid primary amide. [PMID:10079066, PMID:15952893]