zinc-methionine and Poultry-Diseases

The influence of diets containing Zn-Met on in vitro and in vivo uptake of Escherichia coli by the mononuclear-phagocytic system was evaluated. Female Nicholas turkeys reared in battery brooders were supplemented with 40 micrograms Zn/g as Zn-Met in a corn soybean meal diet from 1 to 3 wk of age. Chemical analysis of the basal diets indicated that the basal diets contained 130 micrograms Zn/g and the Zn-Met diets contained 165 micrograms Zn/g. Each diet was fed to three replicate pens of 8 birds in Experiment 1 and three pens of 16 birds in Experiment 2. Body weight gain, feed conversion (FC), and clearance of injected E. coli from blood were determined in Experiments 1 and 2. Abdominal exudate cells (AEC) were recruited by intra-abdominal Sephadex injection. Substrate adherence potential and incidence of macrophages in AEC, phagocytosis of E. coli in vitro in terms of percentage phagocytic macrophages, and number of internalized E. coli per phagocytic macrophage, were quantified in Experiment 1. Plasma Zn concentrations and plasma alkaline phosphatase activity (ALKP) were determined in Experiment 2. Supplemental Zn-Met improved 3-wk BW gain (P < or = .003) only in Experiment 2. Dietary Zn-Met increased mean adherence of cells by 69% (P < or = .001). The number of phagocytized E. coli per macrophage did not differ significantly between treatments; however, E. coli clearance from blood was significantly improved in poults receiving Zn-Met in Experiment 2. Plasma Zn was higher in poults supplemented with Zn-Met prior to and after E. coli administration (P < or = .02).(ABSTRACT TRUNCATED AT 250 WORDS)

Topics: Alkaline Phosphatase; Animals; Escherichia coli Infections; Female; Food, Fortified; Macrophages; Methionine; Organometallic Compounds; Phagocytosis; Poultry Diseases; Random Allocation; Turkeys; Weight Gain; Zinc

The ability of dietary zinc-methionine (Zn-Met) to enhance mononuclear-phagocytic function against Salmonella arizona and enteritidis was investigated in young turkeys. Feed/gain and body wt gain at 21 d of age were not affected by Zn-Met. The addition of 30 or 45 ppm Zn from Zn-Met to a Zn adequate diet significantly increased cutaneous basophil hypersensitivity to phytohemagglutinin-P. The clearance of intravenously administered S. enteritidis from blood was not affected by 30 ppm of supplemental Zn from Zn-Met. However, 30 ppm Zn from Zn-Met increased the reduction of intravenously administered S. arizona from spleen. Percentages of myeloid and mononuclear-phagocytic cells before and after S. enteritidis infection were not affected by supplemental Zn-Met. Turkeys supplemented with Zn-Met showed enhanced in vitro phagocytosis of S. enteritidis by Sephadex-elicited abdominal exudate cells. The phagocytosis of S. arizona was unaffected by Zn-Met.

Topics: Alkaline Phosphatase; Animals; Basophils; Body Weight; Cell Adhesion; Colony Count, Microbial; Intradermal Tests; Leukocytes, Mononuclear; Liver; Macrophages; Male; Methionine; Organometallic Compounds; Phagocytes; Phytohemagglutinins; Poultry Diseases; Random Allocation; Salmonella; Salmonella enteritidis; Salmonella Infections, Animal; Skin; Spleen; Turkeys; Zinc

The effects of zinc methionine product (ZP) supplementation to Single Comb White Leghorn hens on egg production and quality were measured through three consecutive egg laying cycles. During the first and second lay cycles, ZP had minor or nonsignificant effects upon hen performance. During the third lay cycle, a low dietary Ca (.3% Ca) stress of 1 mo duration was encountered. During this low-Ca stress period, hens fed 1 g ZP/kg produced the greatest number of eggs (P less than .05), and during recovery from that low-Ca stress, the hens receiving 2 g ZP/kg produced the most eggs (P less than .05). The ZP appeared to help hens maintain egg size throughout this stress period. The present results indicate that ZP was beneficial to hens during low-Ca stress and during the recovery period following that stress.

Topics: Animals; Calcium; Chickens; Egg Shell; Eggs; Female; Methionine; Organometallic Compounds; Oviposition; Poultry Diseases; Stress, Physiological; Zinc