zhengguangmycin and Tongue-Neoplasms

The purpose of this study was to determine the effect of transient exposure to chemotherapeutic drugs on multidrug resistance of Tca8113 cells.. The MDR1 and MRP gene expressions in Tca8113 and K562/ADM cells lines were analyzed using reverse transcription polymerase chain reaction (RT-PCR), after the cells were treated with different cytotoxic drugs. The function and expressions of P-glycoprotein 170 and multidrug resistant associated protein were studied using fluorescence photometric assays.. The inhibitive rate of Tca8113 cells was higher than that of K562/ADM, after exposure to chemotherapeutic drugs. The transient exposure to cytotoxic drugs weakly induced MDR1 and multidrug resistant associated protein expression in Tca8113 cells. The intracellular drug concentration in K562/ADM was lower than that in Tca8113 cells.. Induction of MDR1 and multidrug resistant associated protein gene expression response to cytotoxic drugs may be related with the increased multidrug resistance in drug-treated human tumor cells.

Topics: Antineoplastic Agents; ATP Binding Cassette Transporter, Subfamily B, Member 1; Bleomycin; Carcinoma, Squamous Cell; Cisplatin; Drug Resistance, Multiple; Drug Resistance, Neoplasm; Genes, MDR; Humans; K562 Cells; Methotrexate; Multidrug Resistance-Associated Proteins; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger; Tongue Neoplasms; Tumor Cells, Cultured

To study and evaluate the clinical effects of combined preoperative chemotherapy and their relations with multi drug resistance (MDR).. 102 cases with oral squamous cell carcinoma(OSCC) were included in the study (63 males and 39 females, aged 22 to 67 years). Among the subjects there were 57 cases with cancer of tongue and 45 cases with cancer of buccal mucosa. 27 cases in the group were classified as stage II, 55 as stage III and 20 cases as stage IV according to TNM standard. All cases accepted PYM + 5-Fu + DDP combined chemotherapy pre-operatively. The total given dose was PYM 48 mg, 5-Fu 7.5 g and DDP 300 mg. After the chemotherapy, radical surgery were performed within 2 weeks. The diagnosis of all cases were proved as OSCC by biopsy.. Total effective rate of the combined chemotherapy was 82.4%. All of the cases were followed up and their 3 years' survival rate was 67.6%.. The combined chemotherapy of PYM + 5-Fu + DDP is effective in using as one of comprehensive treatment for OSCC.

Topics: Adult; Aged; Antineoplastic Combined Chemotherapy Protocols; Bleomycin; Carcinoma, Squamous Cell; Cisplatin; Combined Modality Therapy; Female; Fluorouracil; Humans; Male; Middle Aged; Mouth Neoplasms; Tongue Neoplasms; Treatment Outcome

The purpose of this study was investigate the effect of hyperthemia on multidrug resistance in K562/ADM cell.. The MDR1 (mulitdrug resistance gene) and MRP (multidrug resistant associated gene) gene expressions in Tca8113 and K562/ADM cell lines were analyzed by RT-PCR after treated with different cytotoxic drugs and different temperature (37 degrees C and 41 degrees C). The function and expression of Pgp and MRP were detected by fluorescence photometeric assays.. Inhibition rate of both cells was significantly enhanced by exposure to chemotherapeutic drugs and 41 degrees C temperature; Exposing to 41 degrees C hyperthemia reduced MDR1 and MRP expression and enhanced intracellular drug concentration as well in K562/ADM.. 41 degrees C hyperthemia could effectively enhance the inhibition rate of chemotherapeutic drugs and partially reverse the multidrug resistance. It is suggested that hyperthemia could be used as a method to overcome multidrug resistance.

Topics: Antineoplastic Agents; Bleomycin; Cisplatin; Drug Resistance, Multiple; Drug Resistance, Neoplasm; Genes, MDR; Humans; Hyperthermia, Induced; K562 Cells; Methotrexate; Multidrug Resistance-Associated Proteins; Tongue Neoplasms

The purpose of this study was to investigate the indication and therapeutic effects of Pingyangmycin injection as a primary therapy of lymphangiomas in oral, maxillofacial and cervical region.. A total of 195 patients (106 males and 89 females) with lymphangiomas in oral and maxillofacial regions were treated in the affiliated dental hospital of Sichuan University from May 1990 to December 2000. The patients' ages ranged from 0.5 to 46 years. The tongue was the most commonly involved site, followed by the cheek and the neck. The 200 lymphangiomas (5 patients had 2 lymphangiomas in different sites) underwent the therapy of Pingyangmycin, which was injected as with 1 mg/ml in saline. The total dose of Pingyangmycin ranged from 5 mg to 70 mg and 5 to 58 times, 1 time per 2-4 weeks.. The curative rate of cystic-type lymphangiomas was the highest. Of the 51 cystic lymphangiomas, 110 capillary lymphangiomas, 18 cavernous lymphangiomas and 21 combinations of capillary and cavenous lymphangiomas, the curative rates were respectively 100% (51), 46.36% (51), 16.16% (3) and 19.05% (4), which showed a significant therapeutic effect, respectively. And 40(78.43%), 19(17.27%), 2(11.11%) and 0(0%) of them completely disappeared. There was no serious side effect with Pingyangmycin-injection treatment, such as pulmonary fibrosis.. The treatment of injection of Pingyangmycin is a selective primary method of lymphangiomas, which can reduce the size of lymphangiomas, and make them completely disappeared.

Topics: Adolescent; Adult; Antibiotics, Antineoplastic; Bleomycin; Child; Child, Preschool; Dexamethasone; Drug Administration Schedule; Female; Head and Neck Neoplasms; Humans; Infant; Injections, Intralesional; Lymphangioma; Male; Mouth Neoplasms; Tongue Neoplasms

The inhibitory effects of gamma-interferon (gamma-IFN), pinyanmycin (PYM), and psoralen (PSO) on human tongue cancer HSC3 cells were tested with MTT assay (tetrazolium-based colorimetric assay). The IC50 (drug concentration which causes 50% inhibition of the cell line) values of gamma-IFN, PYM and PSO were 371,535 U/ml, 224 ng/ml and 25,119 ng/ml, respectively. The CI50 (combination index) values of the combinations of PYM with gamma-IFN at the concentrations of 10(2), 10(3), 10(4) and 10(5) U/ml were 0.12, 0.04, 0.05, and 0.29, respectively; and those of PSO with gamma-IFN at the concentrations of 10(2), 10(3), 10(4) and 10(5) U/ml were 1.00, 0.66, 0.30 and 0.28, respectively. The growth inhibition (%) of the HSC3 cells transplanted tumor in nude mice by gamma-IFN, PYM, gamma-IFN plus PYM, PSO, and gamma-IFN plus PSO were 67.6, 103.5, 110.0, 83.1 and 102.9, respectively; the body weight loss (%) of the treated mice were 1.2, 6.9, 0.8, 5.0 and 1.8 respectively, and that of the control was 12.5. The results indicated that the combination effects of gamma-IFN with PYM or gamma-IFN with PSO are synergic and beneficial to the general health of the victim mice.

Topics: Animals; Antibiotics, Antineoplastic; Bleomycin; Drug Synergism; Ficusin; Humans; Interferon-gamma; Mice; Mice, Nude; Neoplasm Transplantation; Tongue Neoplasms; Tumor Cells, Cultured

Topics: Animals; Antibiotics, Antineoplastic; Bleomycin; Carcinoma, Squamous Cell; Combined Modality Therapy; Female; Humans; Immunotherapy, Adoptive; Killer Cells, Lymphokine-Activated; Male; Mice; Mice, Inbred BALB C; Mice, Nude; Tongue Neoplasms; Tumor Cells, Cultured

This was a report on the sensitivity of human squamous (Tca-8113) and adenoid cystic carcinoma (Acc-2) cell lines with Cisplatin (DDP) and it's combined chemotherapy (DDP+VCR+PYM, or PVP). DDP could kill both these two cell lines in vitro depending on the concentration of agent in given time. The rate of 3H-TdR incorporation, plating efficiency and DNA synthesis of carcinoma cells were prevented in drug-exposing groups. Tca-8113 cell line was more sensitive to DDP than Acc-2 cell line (P less than 0.01). PVP combined chemotherapy could enhance DDP's anticancer efficiency.

Topics: Antineoplastic Combined Chemotherapy Protocols; Bleomycin; Carcinoma, Adenoid Cystic; Carcinoma, Squamous Cell; Cisplatin; Humans; Salivary Gland Neoplasms; Tongue Neoplasms; Tumor Cells, Cultured; Vincristine

Topics: Antibiotics, Antineoplastic; Bleomycin; Carcinoma, Squamous Cell; Humans; Hyperbaric Oxygenation; Tongue Neoplasms; Tumor Cells, Cultured

This paper reports an observation on kinetics of induction and resolution of the thermotolerance expressed by Tca8113 in vitro in form of colony-forming rate, synchronization and laser flow cytometry after the cells being exposed to continuous and fractionized heat treatment of 41.5-45.5 degrees C for 4 hours. The process of thermotolerance kinetics could be divided into three phases: triggering, development and decay. The triggering phase lasted about 8 hours. The duration of development and decay phases was related to the temperature of initial heating. The higher the temperature, the shorter the duration. Generally, thermotolerance gradually disappeared 96 hours after the initial heating treatment. The thermotolerance of the synchronized cells in various phases was G1 greater than G2 greater than M greater than S. Hyperthermia simultaneously combined with pingyangmycin both produces a greater synergetic cytotoxic effect and reduces the degree of thermotolerance.

Topics: Antibiotics, Antineoplastic; Bleomycin; Carcinoma, Squamous Cell; Cell Cycle; Cell Line; Hot Temperature; Humans; Tongue Neoplasms

Topics: Antibiotics, Antineoplastic; Bleomycin; Carcinoma, Squamous Cell; Esophageal Neoplasms; Head and Neck Neoplasms; Humans; Lip Neoplasms; Tongue Neoplasms