zhengguangmycin and Laryngeal-Neoplasms

We have successfully established a human laryngeal squamous carcinoma model in nude mice by transplantation of surgical specimen from patients with laryngeal carcinoma. Histopathological study revealed squamous carcinoma. We named it HLC14. Light and electron microscopy showed that the morphology was similar to that of the original tumor. The transplanted tumor has maintained for more than two years through 25 passages. The tumor grew steadily and fast. The successful rate of transplantation was 100%. Pingyangmycine was then used to treat the transplantable model. The result was satisfactory.

Topics: Animals; Antibiotics, Antineoplastic; Bleomycin; Carcinoma, Squamous Cell; Humans; Laryngeal Neoplasms; Mice; Mice, Inbred BALB C; Mice, Nude; Neoplasm Transplantation; Tumor Cells, Cultured

Lymphocytes from 28 untreated laryngeal cancer patients and 23 healthy controls were cultured in vitro and exposured to pingyangmycin (bleomycin A5), a clastogen. The lymphocytes were arrested in metaphase and analyzed. The total chromosome aberration rate, mean chromatid break rate per cell and cell aberration rate were 1.98% +/- 0.05%, 0.57% +/- 0.35%, and 42.8% +/- 12% respectively for laryngeal cancer patients. However, for healthy controls these values were 0.94% +/- 0.04%, 0.28% +/- 0.12%, and 27% +/- 12% respectively. Statistical analysis showed there are significant differences between the two groups. The data indicate that under our experimental conditions chromatid break rate 0.40 can be considered to be a borderline value, 0.80 hypersensitive value. For any individual, if the chromatid break rate is 0.40 or more, one should be ranked as having cancer risk. If 0.80 or more, then, highly cancer risk.

Topics: Adult; Aged; Antibiotics, Antineoplastic; Bleomycin; Carcinoma, Squamous Cell; Chromosome Aberrations; Female; Humans; Laryngeal Neoplasms; Lymphocytes; Male; Middle Aged; Mutagens; Tumor Cells, Cultured