yo-pro-1 and Lymphoma--B-Cell

yo-pro-1 has been researched along with Lymphoma--B-Cell* in 1 studies

Other Studies

1 other study(ies) available for yo-pro-1 and Lymphoma--B-Cell

Article	Year
Rapid quantification of cell viability and apoptosis in B-cell lymphoma cultures using cyanine SYTO probes. Methods in molecular biology (Clifton, N.J.), 2011, Volume: 740 The gross majority of classical apoptotic hallmarks can be rapidly examined by multiparameter flow cytometry. As a result, cytometry became a technology of choice in diverse studies of cellular demise. In this context, a novel class of substituted unsymmetrical cyanine SYTO probes has recently become commercially available. Derived from thiazole orange, SYTO display low intrinsic fluorescence, with strong enhancement upon binding to DNA and/or RNA. Broad selection of excitation/emission spectra has recently driven implementation of SYTO dyes in polychromatic protocols with the detection of apoptosis being one of the most prominent applications In this chapter, we outline a handful of commonly used protocols for the assessment of apoptotic events using selected SYTO probes (SYTO 16, 62, 80) in conjunction with common plasma membrane permeability markers (PI, YO-PRO 1, 7-AAD). Topics: Apoptosis; Benzoxazoles; Cell Survival; Cytological Techniques; Dactinomycin; Fluorescent Dyes; Humans; Lymphoma, B-Cell; Molecular Probes; Propidium; Quinolinium Compounds; Tumor Cells, Cultured	2011

Article

Year

Rapid quantification of cell viability and apoptosis in B-cell lymphoma cultures using cyanine SYTO probes.

Methods in molecular biology (Clifton, N.J.), 2011, Volume: 740

The gross majority of classical apoptotic hallmarks can be rapidly examined by multiparameter flow cytometry. As a result, cytometry became a technology of choice in diverse studies of cellular demise. In this context, a novel class of substituted unsymmetrical cyanine SYTO probes has recently become commercially available. Derived from thiazole orange, SYTO display low intrinsic fluorescence, with strong enhancement upon binding to DNA and/or RNA. Broad selection of excitation/emission spectra has recently driven implementation of SYTO dyes in polychromatic protocols with the detection of apoptosis being one of the most prominent applications In this chapter, we outline a handful of commonly used protocols for the assessment of apoptotic events using selected SYTO probes (SYTO 16, 62, 80) in conjunction with common plasma membrane permeability markers (PI, YO-PRO 1, 7-AAD).

Topics: Apoptosis; Benzoxazoles; Cell Survival; Cytological Techniques; Dactinomycin; Fluorescent Dyes; Humans; Lymphoma, B-Cell; Molecular Probes; Propidium; Quinolinium Compounds; Tumor Cells, Cultured

2011