ym-254890 and HIV-Infections

Dopaminergic dysfunction has long been connected to the development of HIV infection in the CNS. Our previous data showed that dopamine increases HIV infection in human macrophages by increasing the susceptibility of primary human macrophages to HIV entry through stimulation of both D1-like and D2-like receptors. These data suggest that, in macrophages, both dopamine receptor subtypes may act through a common signaling mechanism. To define better the mechanism(s) underlying this effect, this study examines the specific signaling processes activated by dopamine in primary human monocyte-derived macrophages (hMDM). In addition to confirming that the increase in entry is unique to dopamine, these studies show that dopamine increases HIV entry through a PKA insensitive, Ca

Topics: Adult; Calcium; Calcium Signaling; Dopamine; Female; Healthy Volunteers; HIV; HIV Infections; Humans; Macrophages; Male; Peptides, Cyclic; Phosphorylation; Primary Cell Culture; Protein Kinase C; Receptors, Dopamine D1; Signal Transduction