yersiniabactin and Plague

yersiniabactin has been researched along with Plague* in 8 studies

Reviews

1 review(s) available for yersiniabactin and Plague

ArticleYear
Yersiniabactin iron uptake: mechanisms and role in Yersinia pestis pathogenesis.
    Microbes and infection, 2011, Volume: 13, Issue:10

    Yersiniabactin (Ybt) is a siderophore-dependent iron uptake system encoded on a pathogenicity island that is widespread among pathogenic bacteria including the Yersiniae. While biosynthesis of the siderophore has been elucidated, the secretion mechanism and a few components of the uptake/utilization pathway are unidentified. ybt genes are transcriptionally repressed by Fur but activated by YbtA, likely in combination with the siderophore itself. The Ybt system is essential for the ability of Yersinia pestis to cause bubonic plague and important in pneumonic plague as well. However, the ability to cause fatal septicemic plague is independent of Ybt.

    Topics: Gene Expression Regulation, Bacterial; Humans; Iron; Phenols; Plague; Thiazoles; Virulence Factors; Yersinia pestis

2011

Other Studies

7 other study(ies) available for yersiniabactin and Plague

ArticleYear
Droplet Tn-Seq identifies the primary secretion mechanism for yersiniabactin in Yersinia pestis.
    EMBO reports, 2023, 10-09, Volume: 24, Issue:10

    Nutritional immunity includes sequestration of transition metals from invading pathogens. Yersinia pestis overcomes nutritional immunity by secreting yersiniabactin to acquire iron and zinc during infection. While the mechanisms for yersiniabactin synthesis and import are well-defined, those responsible for yersiniabactin secretion are unknown. Identification of this mechanism has been difficult because conventional mutagenesis approaches are unable to inhibit trans-complementation by secreted factors between mutants. To overcome this obstacle, we utilized a technique called droplet Tn-seq (dTn-seq), which uses microfluidics to isolate individual transposon mutants in oil droplets, eliminating trans-complementation between bacteria. Using this approach, we first demonstrated the applicability of dTn-seq to identify genes with secreted functions. We then applied dTn-seq to identify an AcrAB efflux system as required for growth in metal-limited conditions. Finally, we showed this efflux system is the primary yersiniabactin secretion mechanism and required for virulence during bubonic and pneumonic plague. Together, these studies have revealed the yersiniabactin secretion mechanism that has eluded researchers for over 30 years and identified a potential therapeutic target for bacteria that use yersiniabactin for metal acquisition.

    Topics: Bacterial Proteins; Humans; Metals; Phenols; Plague; Thiazoles; Yersinia pestis

2023
Yersiniabactin contributes to overcoming zinc restriction during
    Proceedings of the National Academy of Sciences of the United States of America, 2021, 11-02, Volume: 118, Issue:44

    Topics: Animals; ATP-Binding Cassette Transporters; Female; Gene Expression; Gene Expression Regulation, Bacterial; Iron; Male; Mice; Mice, 129 Strain; Mice, Inbred C57BL; Phenols; Plague; Siderophores; Thiazoles; Virulence; Virulence Factors; Yersinia pestis; Zinc

2021
The Yersinia pestis siderophore, yersiniabactin, and the ZnuABC system both contribute to zinc acquisition and the development of lethal septicaemic plague in mice.
    Molecular microbiology, 2014, Volume: 93, Issue:4

    Bacterial pathogens must overcome host sequestration of zinc (Zn(2+) ), an essential micronutrient, during the infectious disease process. While the mechanisms to acquire chelated Zn(2+) by bacteria are largely undefined, many pathogens rely upon the ZnuABC family of ABC transporters. Here we show that in Yersinia pestis, irp2, a gene encoding the synthetase (HMWP2) for the siderophore yersiniabactin (Ybt) is required for growth under Zn(2+) -deficient conditions in a strain lacking ZnuABC. Moreover, growth stimulation with exogenous, purified apo-Ybt provides evidence that Ybt may serve as a zincophore for Zn(2+) acquisition. Studies with the Zn(2+) -dependent transcriptional reporter znuA::lacZ indicate that the ability to synthesize Ybt affects the levels of intracellular Zn(2+) . However, the outer membrane receptor Psn and TonB as well as the inner membrane (IM) ABC transporter YbtPQ, which are required for Fe(3+) acquisition by Ybt, are not needed for Ybt-dependent Zn(2+) uptake. In contrast, the predicted IM protein YbtX, a member of the Major Facilitator Superfamily, was essential for Ybt-dependent Zn(2+) uptake. Finally, we show that the ZnuABC system and the Ybt synthetase HMWP2, presumably by Ybt synthesis, both contribute to the development of a lethal infection in a septicaemic plague mouse model.

    Topics: Animals; ATP-Binding Cassette Transporters; Disease Models, Animal; Mice; Phenols; Plague; Sepsis; Thiazoles; Virulence; Virulence Factors; Yersinia pestis; Zinc

2014
The yersiniabactin transport system is critical for the pathogenesis of bubonic and pneumonic plague.
    Infection and immunity, 2010, Volume: 78, Issue:5

    Iron acquisition from the host is an important step in the pathogenic process. While Yersinia pestis has multiple iron transporters, the yersiniabactin (Ybt) siderophore-dependent system plays a major role in iron acquisition in vitro and in vivo. In this study, we determined that the Ybt system is required for the use of iron bound by transferrin and lactoferrin and examined the importance of the Ybt system for virulence in mouse models of bubonic and pneumonic plague. Y. pestis mutants unable to either transport Ybt or synthesize the siderophore were both essentially avirulent via subcutaneous injection (bubonic plague model). Surprisingly, via intranasal instillation (pneumonic plague model), we saw a difference in the virulence of Ybt biosynthetic and transport mutants. Ybt biosynthetic mutants displayed an approximately 24-fold-higher 50% lethal dose (LD(50)) than transport mutants. In contrast, under iron-restricted conditions in vitro, a Ybt transport mutant had a more severe growth defect than the Ybt biosynthetic mutant. Finally, a Delta pgm mutant had a greater loss of virulence than the Ybt biosynthetic mutant, indicating that the 102-kb pgm locus encodes a virulence factor, in addition to Ybt, that plays a role in the pathogenesis of pneumonic plague.

    Topics: Animals; Female; Iron; Lethal Dose 50; Mice; Phenols; Plague; Survival Analysis; Thiazoles; Virulence; Virulence Factors; Yersinia pestis

2010
Role of the Yersinia pestis yersiniabactin iron acquisition system in the incidence of flea-borne plague.
    PloS one, 2010, Dec-17, Volume: 5, Issue:12

    Plague is a flea-borne zoonosis caused by the bacterium Yersinia pestis. Y. pestis mutants lacking the yersiniabactin (Ybt) siderophore-based iron transport system are avirulent when inoculated intradermally but fully virulent when inoculated intravenously in mice. Presumably, Ybt is required to provide sufficient iron at the peripheral injection site, suggesting that Ybt would be an essential virulence factor for flea-borne plague. Here, using a flea-to-mouse transmission model, we show that a Y. pestis strain lacking the Ybt system causes fatal plague at low incidence when transmitted by fleas. Bacteriology and histology analyses revealed that a Ybt-negative strain caused only primary septicemic plague and atypical bubonic plague instead of the typical bubonic form of disease. The results provide new evidence that primary septicemic plague is a distinct clinical entity and suggest that unusual forms of plague may be caused by atypical Y. pestis strains.

    Topics: Animals; Female; Immune System; Iron; Lymph Nodes; Mice; Mutation; Phenols; Plague; Siphonaptera; Species Specificity; Thiazoles; Virulence Factors; Yersinia pestis

2010
YbtP and YbtQ: two ABC transporters required for iron uptake in Yersinia pestis.
    Molecular microbiology, 1999, Volume: 32, Issue:2

    Yersinia pestis, the causative agent of plague, makes a siderophore termed yersiniabactin (Ybt), which it uses to obtain iron during growth at 37 degrees C. The genes required for the synthesis and utilization of Ybt are located within a large, unstable region of the Y. pestis chromosome called the pgm locus. Within the pgm locus, just upstream of a gene (ybtA) that regulates expression of the Ybt receptor and biosynthetic genes, is an operon consisting of 4 genes - ybtP, ybtQ, ybtX and ybtS. Transcription of the ybtPQXS operon is repressed by Fur and activated by YbtA. The product of ybtX is predicted to be an exceedingly hydrophobic cytoplasmic membrane protein that does not appear to contribute any vital function to Ybt biosynthesis or utilization in vitro. ybtP and ybtQ encode putative members of the traffic ATPase/ABC transporter family. YbtP and YbtQ are structurally unique among the subfamily of ABC transporters associated with iron transport, in that they both contain an amino-terminal membrane-spanning domain and a carboxy-terminal ATPase. Cells with mutations in ybtP or ybtQ still produced Ybt but were impaired in their ability to grow at 37 degrees C under iron-deficient conditions, indicating that YbtP and YbtQ are needed for iron uptake. In addition, a ybtP mutant showed reduced iron accumulation and was avirulent in mice by a subcutaneous route of infection that mimics flea transmission of bubonic plague.

    Topics: Amino Acid Sequence; Animals; ATP-Binding Cassette Transporters; Bacterial Proteins; Base Sequence; beta-Galactosidase; Chromosome Mapping; Gene Expression Regulation, Bacterial; Genes, Bacterial; Iron; Mice; Molecular Sequence Data; Operon; Phenols; Plague; Promoter Regions, Genetic; Sequence Analysis, DNA; Siderophores; Thiazoles; Virulence; Yersinia pestis

1999
Iron acquisition in plague: modular logic in enzymatic biogenesis of yersiniabactin by Yersinia pestis.
    Chemistry & biology, 1998, Volume: 5, Issue:10

    Virulence in the pathogenic bacterium Yersinia pestis, causative agent of bubonic plague, has been correlated with the biosynthesis and transport of an iron-chelating siderophore, yersiniabactin, which is induced under iron-starvation conditions. Initial DNA sequencing suggested that this system is highly conserved among the pathogenic Yersinia. Yersiniabactin contains a phenolic group and three five-membered thiazole heterocycles that serve as iron ligands.. The entire Y. pestis yersiniabactin region has been sequenced. Sequence analysis of yersiniabactin biosynthetic regions (irp2-ybtE and ybtS) reveals a strategy for siderophore production using a mixed polyketide synthase/nonribosomal peptide synthetase complex formed between HMWP1 and HMWP2 (encoded by irp1 and irp2). The complex contains 16 domains, five of them variants of phosphopantetheine-modified peptidyl carrier protein or acyl carrier protein domains. HMWP1 and HMWP2 also contain methyltransferase and heterocyclization domains. Mutating ybtS revealed that this gene encodes a protein essential for yersiniabactin synthesis.. The HMWP1 and HMWP2 domain organization suggests that the yersiniabactin siderophore is assembled in a modular fashion, in which a series of covalent intermediates are passed from the amino terminus of HMWP2 to the carboxyl terminus of HMWP1. Biosynthetic labeling studies indicate that the three yersiniabactin methyl moieties are donated by S-adenosylmethionine and that the linker between the thiazoline and thiazolidine rings is derived from malonyl-CoA. The salicylate moiety is probably synthesized using the aromatic amino-acid biosynthetic pathway, the final step of which converts chorismate to salicylate. YbtS might be necessary for converting chorismate to salicylate.

    Topics: Amino Acid Sequence; Bacterial Outer Membrane Proteins; Bacterial Proteins; Base Sequence; DNA Primers; Iron; Iron-Binding Proteins; Molecular Sequence Data; Multienzyme Complexes; Periplasmic Binding Proteins; Phenols; Plague; Salicylic Acid; Sequence Homology, Amino Acid; Siderophores; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Thiazoles; Virulence; Yersinia pestis

1998