yersiniabactin and Enterobacteriaceae-Infections

yersiniabactin has been researched along with Enterobacteriaceae-Infections* in 2 studies

Other Studies

2 other study(ies) available for yersiniabactin and Enterobacteriaceae-Infections

Article	Year
Yersiniabactin and other siderophores produced by clinical isolates of Enterobacter spp. and Citrobacter spp. FEMS immunology and medical microbiology, 2004, Jan-15, Volume: 40, Issue:1 We analyzed the ability of extraintestinal strains of Enterobacter spp. and Citrobacter spp. to employ different siderophore-mediated strategies of iron acquisition. All strains produced iron-chelating compounds. Cross-feeding assays indicated that most isolates of both Enterobacter spp. and Citrobacter spp. excreted catecholate siderophore enterobactin, less produced aerobactin, and single strains excreted hydroxamates different from aerobactin. Besides, we analyzed if the strains had the ability to produce the siderophore yersiniabactin coded by the Yersinia high-pathogenicity island (HPI). The presence of HPI genes was observed in single isolates of three species: E. cloaceae, E. aerogenes and C. koseri. A detailed polymerase chain reaction analysis revealed differences in the genetic organization of the HPIs; however, in a cross-feeding test we proved that yersiniabactin was produced and the island was functional. Topics: Bacterial Proteins; Chelating Agents; Citrobacter; DNA, Bacterial; Enterobacter; Enterobacteriaceae Infections; Genomic Islands; Humans; Hydroxamic Acids; Iron; Phenols; Polymerase Chain Reaction; Siderophores; Thiazoles; Virulence; Yersinia	2004
Siderophore-mediated strategies of iron acquisition by extraintestinal isolates of Enterobacter spp. Acta microbiologica Polonica, 2003, Volume: 52, Issue:1 A total of 89 examined Enterobacter isolates belonging to three species: E. cloaceae, E. aerogenes and E. sakazakii, produced iron chelators detected in universal CAS assay. In chemical assays the strains were shown to excrete mostly catecholate (88 strains) and hydroxamate (42 strains) type of siderophores. Forty-one strains produced both catecholate and hydroxamate siderophores whereas one isolate produced only hydroxamate. Besides, the isolates were screened for genes coding for another siderophore: yersiniabactin. The genes for biosynthesis and uptake of yersiniabactin are located on the high-pathogenicity island (HPI) of Yersinia spp. The presence of three marker genes irp1, irp2 and fyuA was estimated by polymerase chain reaction. Two strains: E. aerogenes and E. cloaceae possessed irp1, irp2 and fyuA genes. PCR products of irp1, irp2 and fyuA were of 240, 280 and 780 bp, respectively. Topics: DNA, Bacterial; Enterobacter; Enterobacteriaceae Infections; Humans; Iron; Phenols; Polymerase Chain Reaction; Siderophores; Thiazoles	2003

Article

Year

Yersiniabactin and other siderophores produced by clinical isolates of Enterobacter spp. and Citrobacter spp.

FEMS immunology and medical microbiology, 2004, Jan-15, Volume: 40, Issue:1

We analyzed the ability of extraintestinal strains of Enterobacter spp. and Citrobacter spp. to employ different siderophore-mediated strategies of iron acquisition. All strains produced iron-chelating compounds. Cross-feeding assays indicated that most isolates of both Enterobacter spp. and Citrobacter spp. excreted catecholate siderophore enterobactin, less produced aerobactin, and single strains excreted hydroxamates different from aerobactin. Besides, we analyzed if the strains had the ability to produce the siderophore yersiniabactin coded by the Yersinia high-pathogenicity island (HPI). The presence of HPI genes was observed in single isolates of three species: E. cloaceae, E. aerogenes and C. koseri. A detailed polymerase chain reaction analysis revealed differences in the genetic organization of the HPIs; however, in a cross-feeding test we proved that yersiniabactin was produced and the island was functional.

Topics: Bacterial Proteins; Chelating Agents; Citrobacter; DNA, Bacterial; Enterobacter; Enterobacteriaceae Infections; Genomic Islands; Humans; Hydroxamic Acids; Iron; Phenols; Polymerase Chain Reaction; Siderophores; Thiazoles; Virulence; Yersinia

2004

Siderophore-mediated strategies of iron acquisition by extraintestinal isolates of Enterobacter spp.

Acta microbiologica Polonica, 2003, Volume: 52, Issue:1

A total of 89 examined Enterobacter isolates belonging to three species: E. cloaceae, E. aerogenes and E. sakazakii, produced iron chelators detected in universal CAS assay. In chemical assays the strains were shown to excrete mostly catecholate (88 strains) and hydroxamate (42 strains) type of siderophores. Forty-one strains produced both catecholate and hydroxamate siderophores whereas one isolate produced only hydroxamate. Besides, the isolates were screened for genes coding for another siderophore: yersiniabactin. The genes for biosynthesis and uptake of yersiniabactin are located on the high-pathogenicity island (HPI) of Yersinia spp. The presence of three marker genes irp1, irp2 and fyuA was estimated by polymerase chain reaction. Two strains: E. aerogenes and E. cloaceae possessed irp1, irp2 and fyuA genes. PCR products of irp1, irp2 and fyuA were of 240, 280 and 780 bp, respectively.

Topics: DNA, Bacterial; Enterobacter; Enterobacteriaceae Infections; Humans; Iron; Phenols; Polymerase Chain Reaction; Siderophores; Thiazoles

2003