warfarin and Vitamin-D-Deficiency

It has been reported that vitamin K deficiency in the rat markedly increases the 1,25-dihydroxyvitamin D3 receptor (VDR) binding to DNA and that vitamin K-dependent gamma-carboxylation of endogenous substrates of the intestinal and renal cytosol, also containing VDR, sharply reduced that binding (Sergeev, I.N., and Spirichev, V.B. (1989) Nutr. Res. 9, 725-733). In the present study we have evaluated vitamin K-dependent 14CO2 incorporation to VDR quantitated by immunoprecipitation with anti-VDR monoclonal antibodies. The results obtained strongly suggest that VDR in vitro can undergo gamma-carboxylation in the presence of vitamin K1 and that 15-25% of Glu residues in the VDR are carboxylated in vivo. Taking into account our earlier findings, it is likely that the VDR gamma-carboxylation modulates its binding to DNA.

Topics: Animals; Antibodies, Monoclonal; Calcitriol; Carbon Dioxide; Carbon Radioisotopes; Chickens; Cholecalciferol; Cytosol; Intestinal Mucosa; Kidney; Male; Receptors, Calcitriol; Receptors, Steroid; Vitamin D Deficiency; Vitamin K; Warfarin