waixenicin-a and Breast-Neoplasms

Transient receptor potential melastatin 7 (TRPM7) plays a role in a number of physiological and pharmacological functions in variety of cells. The aim of this study was to clarify the role for TRPM7 channels and the effect of waixenicin A on the pacemaking activity of interstitial cells of Cajal (ICCs) and on the cell viability of the human gastric and breast adenocarcinoma cell lines, AGS and MCF-7, respectively. Waixenicin A decreased the amplitude of pacemaker potentials in cultured ICC clusters and inhibited TRPM7 currents, but had no effect on Ca(2+) -activated Cl(-) conductance (ANO1). Furthermore, waixenicin A was found to inhibit the growth and survival of AGS and MCF-7 cells. These findings indicate that TRPM7 channel modulates intestinal motility and regulates the pathophysiology of human gastric and breast adenocarcinoma cells. These findings suggest that TRPM7 channel be considered a potential target for the treatment of gut motor disorders and gastric and breast cancer.

Topics: Acetates; Adenocarcinoma; Animals; Breast Neoplasms; Cell Line, Tumor; Cell Survival; Diterpenes; Female; Gastrointestinal Motility; Humans; Interstitial Cells of Cajal; Male; Membrane Potentials; Mice; Mice, Inbred BALB C; Protein Serine-Threonine Kinases; Stomach Neoplasms; TRPM Cation Channels