vuf-8430 and Spinal-Cord-Injuries

Neuropathic pain is under-treated, with a detrimental effect on quality of life, partly because of low treatment efficacy, but also because pathophysiological mechanisms are not fully elucidated. To clarify the pathobiology of neuropathic pain, we studied the contribution of neuroinflammation and oxidative stress in a model of peripheral neuropathy. We also assessed an innovative treatment for neuropathic pain by investigating the effects of histamine H. A peripheral mononeuropathy was induced in mice, by spared nerve injury (SNI). Neuroinflammation and oxidative stress parameters were evaluated by spectrophotometry. The mechanical (von Frey test) and thermal (plantar test) nociceptive thresholds were evaluated.. SNI mice showed increased expression of the pro-inflammatory cytokines IL-1ß and TNF-α, decreased antioxidant enzyme Mn-containing SOD (MnSOD), increased levels of 8-hydroxy-2'-deoxyguanosine (8-OHdG), an indicator of oxidative DNA damage, and of PARP, nuclear enzyme activated upon DNA damage. Intrathecal administration of VUF 8430 (H. In the SNI mouse model of neuropathic pain, neuronal H

Topics: Animals; Dose-Response Relationship, Drug; Guanidines; Histamine; Inflammation; Male; Mice; Neuralgia; Oxidative Stress; Receptors, G-Protein-Coupled; Receptors, Histamine; Receptors, Histamine H4; Spinal Cord Injuries; Structure-Activity Relationship; Thiourea