vpm-chloride and Shock--Septic

To investigate the contribution of neutrophil activation as innate immune cells during septic shock-induced disseminated intravascular coagulation.. Prospective study.. One University Hospital ICU.. Hundred patients with septic shock. Thirty-five patients had disseminated intravascular coagulation according to Japanese Association for Acute Medicine 2006 score.. None.. Neutrophil chromatin decondensation was assessed by measuring neutrophil fluorescence (NEUT-side-fluorescence light) labeled by a fluorochrome-based polymethine reagent using a routine automated flow cytometer Sysmex XN20 (Sysmex, Kobe, Japan) and neutrophil-derived CD66b microparticles by prothrombinase assay. Measurements in disseminated intravascular coagulation and no disseminated intravascular coagulation patients showed that a mean value of NEUT-side-fluorescence light above 57.3 arbitrary units had a sensitivity of 90.91% and a specificity of 80.60% for disseminated intravascular coagulation diagnosis. NEUT-side-fluorescence light was correlated to the CD66b microparticles/neutrophil count, a surrogate of neutrophil activation associated with septic shock-induced disseminated intravascular coagulation.. NEUT-side-fluorescence light, routinely available, could prove an accurate biomarker of neutrophil activation.

Topics: Cell Count; Disseminated Intravascular Coagulation; Flow Cytometry; Fluorescence; Humans; Indoles; Intensive Care Units; Neutrophil Activation; Neutrophils; Prospective Studies; Shock, Septic