vitamin-d-2 has been researched along with Xeroderma-Pigmentosum* in 1 studies
1 other study(ies) available for vitamin-d-2 and Xeroderma-Pigmentosum
| Article | Year |
|---|---|
Simplified method for the determination of 25-hydroxy and 1alpha,25-dihydroxy metabolites of vitamins D2 and D3 in human plasma. Application to nutritional studies.
A simplified method for the determination of 25-hydroxy and 1alpha,25-dihydroxy metabolites of vitamins D2 and D3 in human plasma was developed. Plasma samples were deproteinizated and applied to a Bond Elut C18OH cartridge to separate 25-hydroxyvitamin D (25-OH-D) and 1alpha,25-dihydroxyvitamin D [1,25(OH)2D] fractions. The 25-OH-D fraction was purified by a Bond Elut C18 cartridge and 25-OH-D2 and 25-OH-D3 were assayed by HPLC using a Zorbax SIL column. The 1,25(OH)2D fraction obtained above was subsequently applied to HPLC using a Zorbax SIL column to separate 1,25(OH)2D2 and 1,25(OH)2D3 fractions which were determined by a radioreceptor assay (RRA) using calf thymus receptor. The method was applied to nutritional studies. Topics: 25-Hydroxyvitamin D 2; Adult; Aged; Calcifediol; Calcitriol; Chromatography, High Pressure Liquid; Ergocalciferols; Food, Fortified; Humans; Hydroxycholecalciferols; Infant; Middle Aged; Xeroderma Pigmentosum | 1997 |