vitamin-b-12 and Waldenstrom-Macroglobulinemia

Topics: Anemia, Aplastic; Anemia, Hemolytic; Anemia, Hemolytic, Congenital; Anemia, Hypochromic; Anemia, Pernicious; Anticoagulants; Chelating Agents; Erythroblastosis, Fetal; Exchange Transfusion, Whole Blood; Female; Fibrinolysis; Folic Acid Deficiency; Hematologic Diseases; Hemoglobinopathies; Hemophilia A; Hemophilia B; Humans; Infant, Newborn; Iron; Leukemia; Pregnancy; Splenectomy; Vitamin B 12; Vitamin B 12 Deficiency; Waldenstrom Macroglobulinemia

The human ileal intrinsic factor receptor was solubilized with Triton X-100 using an improved method originally devised for the porcine receptor. At pH 7.4 and in the presence of Ca2+ the receptor bound the vitamin B12 complexes of normal human and pig intrinsic factor but not that of an abnormal biologically inert human intrinsic factor. EGTA dissociated vitamin B12-intrinsic factor from the receptor complexes. The solubilized vitamin B12-intrinsic factor receptor complex consisted of three to four molecular species termed HC-L, HC-20 S, HC-12 S and HC-8.5 S (the three last-mentioned referring to sedimentation coefficients). Of these HC-20 S was the dominating component and had a Stokes radius of 18 nm. Radioactive calcium was shown to be bound to vitamin B12-intrinsic factor and to be contained in its complex with the receptor.

Topics: Animals; Calcium; Cobalt Radioisotopes; Gastric Juice; Humans; Ileum; Intestinal Mucosa; Intrinsic Factor; Receptors, Drug; Solubility; Swine; Vitamin B 12; Waldenstrom Macroglobulinemia

Topics: Anemia; Anemia, Macrocytic; Anemia, Pernicious; Blood Cell Count; Blood Protein Electrophoresis; Calcium; Drug Therapy; Geriatrics; Humans; Immunoelectrophoresis; Iron; Multiple Myeloma; Osteoporosis; Plasmacytoma; Pneumonia; Vitamin B 12; Vitamin B 12 Deficiency; Waldenstrom Macroglobulinemia