vitamin-b-12 and Polycythemia-Vera

Topics: Alpha-Globulins; Blood Proteins; Bone Marrow; Bone Marrow Cells; Carrier Proteins; Cobalt Radioisotopes; Evaluation Studies as Topic; Humans; Leukemia, Myeloid; Leukemia, Myeloid, Acute; Leukocytes; Polycythemia Vera; Primary Myelofibrosis; Protein Binding; Vitamin B 12; Vitamin B 12 Deficiency

Topics: Anemia, Pernicious; Biological Transport; Diagnosis, Differential; Glycoproteins; Humans; Indicators and Reagents; Intrinsic Factor; Polycythemia Vera; Protein Binding; Proteins; Radioisotope Dilution Technique; Terminology as Topic; Vitamin B 12

Topics: Anemia; Anemia, Macrocytic; Blood Platelets; Eosinophilia; Epoetin Alfa; Erythropoietin; Humans; Iron; Leukemia; Polycythemia Vera; Vitamin B 12

To determine if increased cell turnover in chronic myeloproliferative disorders can lead to hyperhomocysteinemia as a result of folate and/or cobalamin depletion, and contribute to oxidative stress.. The clinical role of oxidative stress was investigated by measuring reactive oxygen species (ROS), total antioxidant capacity (TAC), and total homocysteine (tHcy), folate, cobalamin, and holotranscobalamin (HoloTC) levels in 51 chronic myeloproliferative disorders patients (male-to-female ratio: 1.1; median age: 64 years; range, 40-84 years), including 42 with primary myelofibrosis and 9 with post-polycythemia vera myelofibrosis.. Myelofibrotic patients had higher tHcy (p = 0.0201) and an unbalanced oxidative status (higher ROS and lower TAC levels; p < 0.0001) than controls. Presence of diabetes or another neoplasia was associated with higher ROS levels (p < 0.05), splenomegaly, hepatomegaly, and peripheral blasts with lower HoloTC levels (p < 0.005). The most severe forms of myelofibrosis (2-3) were associated with lower TAC (p = 0.045) and HoloTC levels (p = 0.017). Patients with Janus kinase-2 mutations had lower HoloTC levels (p = 0.0059). HoloTC deficiency was more frequently associated with Janus kinase-2 homozygosity (p < 0.0003).. Our findings suggest that the determination of HoloTC, tHcy, ROS concentrations, and TAC, can identify latent cobalamin deficiency and provide a rational basis for correcting the increased oxidation associated with disease progression.

Topics: Adult; Aged; Aged, 80 and over; Antioxidants; Chronic Disease; Female; Folic Acid; Homocysteine; Humans; Janus Kinase 2; Male; Methylenetetrahydrofolate Reductase (NADPH2); Middle Aged; Mutation; Oxidative Stress; Polycythemia Vera; Polymorphism, Single Nucleotide; Primary Myelofibrosis; Reactive Oxygen Species; Transcobalamins; Vitamin B 12

A few simple, low-priced, outpatient investigations, including an upper abdominal ultrasonography, a measurement of the arterial oxygen saturation and, in some cases, a leukocyte alkaline phosphatase score (LAP) suffice to find out the etiology of an increased red cell mass in most patients. The diagnosis of polycythemia vera (PV) could be accepted in patients with an increased red cell mass if the spleen is enlarged and if the arterial oxygen saturation is normal, although the latter measurement may not be required in typical cases. If the spleen is not enlarged, the diagnosis of PV could be accepted if, in addition, the leukocyte or thrombocyte count is increased. An elevated LAP score also points to the diagnosis of PV, provided fever or inflammation are not present. If, at that stage, an etiologic diagnosis has not been made, smoker's polycythemia should be considered and excluded. The next step should include a serum erythropoietin assay and culture of erythroid stem cells before performing investigations aimed at ruling out the many conditions associated with secondary polycythemia.

Topics: Alkaline Phosphatase; Erythrocyte Count; Humans; Leukocytes; Polycythemia Vera; Predictive Value of Tests; Prognosis; Splenomegaly; Vitamin B 12

Topics: Anemia, Macrocytic; Anemia, Megaloblastic; Humans; Male; Middle Aged; Polycythemia Vera; Vitamin B 12

Topics: Aged; Anemia; Anemia, Macrocytic; Blood Cell Count; Blood Transfusion; Chlorambucil; Erythrocyte Indices; Hematocrit; Hematologic Diseases; Hemoglobins; Humans; Leukemia, Lymphoid; Middle Aged; Multiple Myeloma; Platelet Count; Polycythemia Vera; Reticulocytes; Thalassemia; Vitamin B 12

The unsaturated B12 binding capacity (UBBC) of the serum and the binding capacity of each of the 3 vitamin B12 binders--the transcobalamins (TC) I, II and III were determined in 21 patients with polycythaemia vera (PV) during the course of the disease and following treatment, using the recently described charged cellulose filter technique. High serum UBBC due to elevated serum TCIII was found in all patients. TCI was moderately elevated in patients who had leucocytosis with a shift to the left. The changes in serum TCIII and UBBC correlated with the activity of the disease. Chemotherapy resulted in a decrease in TCIII and UBBC. The decrease in TCIII and UBBC folowing chemotherapy may be observed before a decrease in the haematocrit and the leucocyte count occurs. Activation of the disease may be assessed by the elevation of TCIII and UBBC. The onset of acute myeloblastic crisis in 1 patient was associated with a decrease in TCIII and TCI levels and a rise in serum TCII. The determination of TCIII and UBBC may be helpful in differentiating true from secondary polycythaemia.

Topics: Blood Proteins; Busulfan; Humans; Leukemia, Myeloid, Acute; Melphalan; Polycythemia Vera; Protein Binding; Transcobalamins; Vitamin B 12

The R-type vitamin B12-binding protein (cobalophilin) in plasma and serum of patients with myeloproliferative diseases and leukocytosis was studied by gel filtration and isoelectric focusing. The isoproteins composing the cobalophilin were mainly the same in these disorders as in plasma of health subjects. In half the patients with chronic myelogenous leukaemia and a few of those with leukocytosis some of the isoproteins were more acid than those found in healthy subjects. In chronic myelogenous leukaemia the high unsaturated vitamin B12-binding capacity of plasma was due to an increase in the fairly acid isoproteins, whereas in polycythaemia vera and in most cases of leukocytosis there was an increase in the least acid isoproteins.

Topics: Blood Proteins; Humans; Isoelectric Focusing; Leukemia, Myeloid; Leukocytosis; Polycythemia Vera; Protein Binding; Vitamin B 12

A gradient was developed for isoelectric focusing in the pH range 2-5. Cobalophilin (earlier called R proteins or vitamin B12-binding proteins of R-type) was isolated from saliva and amniotic fluid in homogeneous form. It was found to be a glycoprotein with a molecular weight of 59,300-69,100. The preparation from amniotic fluid contained 33% carbohydrate. Cobalophilin variants in plasma, serum, granulocytes, platelets, amniotic fluid, milk, saliva and gastric juice were characterized by isoelectric focusing. Most fluids and cells contained the same isoproteins, with pI values between 2.3 and 5.0. Isoproteins of presumably myelogenic origin (e.g. those in granulocytes and plasma) had pI values below 4.2, whereas those of glandular origin (in milk and saliva) had a pI range of 4.0-5.0. Serum contained more cobalophilin than plasma, owing to release of this protein from granulocytes during clotting. This phenomenon also changed the isoprotein pattern. Plasma and serum from newborn infants and from patients with leucocytosis, polycythaemia vera and chronic myelogenous leukaemia contained the same isoproteins as were found in plasma from healthy subjects. In addition to these, isoproteins with lower than 'normal' pI values were often found in chronic myelogenous leukaemia and occasionally in leucocytosis. It is concluded that cobalophilin from different fluids and cells is a single microheterogeneous protein with a variable carbohydrate composition. The distribution of cobalophilin in different body fluids and cells supports the suggestion that cobalophilin is an antimicrobial protein (Gullberg 1972) like lactoferrin and lysozyme.

Topics: Amniotic Fluid; Blood Platelets; Blood Proteins; Gastric Juice; Glycoproteins; Granulocytes; Humans; Infant, Newborn; Leukemia, Myeloid; Leukocytosis; Milk, Human; Molecular Weight; Polycythemia Vera; Protein Binding; Saliva; Vitamin B 12

The unsaturated vitamin B12 binding capacity of whole serum (UBBC) and of the three transcobalamins (TC) has been studied in patients with various haematological diseases including myeloproliferative disorders (MPD) and acute leukaemia. The binding capacity of TC I and TC III was increased in MPD; TC I being particularly high in chronic granulocytic leukaemia (CGL) and TC III especially raised in polycythaemia rubra vera (PRV) and in infectious leucocytosis. The binding capacity of both TC I and TC III correlated with blood neutrophil count and the ratio TC III/TC I was low in CGL and increased in PRV. TC II was increased in acute myelogenous leukaemia, during remission and blast cell crisis of CGL and in refractory anaemia with excess of myeloblasts but not in acute lymphoblastic leukaemia (ALL). TC II correlated inversely with blood neutrophil count. There is an inverse ratio between TC II and TC I at least in myelogenous leukaemia. These abnormalities are discussed in relation to granulocyte kinetics. TC III and TC I reflect probably the total body granulocyte pool and share some biochemical and immunological properties supporting the view that they have a common origin in the more mature stages of the granulocyte cell line while TC II probably originates partly in more primitive granulocytes.

Topics: Anemia, Aplastic; Blood Cell Count; Blood Proteins; Humans; Leukemia; Leukemia, Lymphoid; Leukemia, Myeloid; Leukocyte Count; Leukocytosis; Myeloproliferative Disorders; Neutrophils; Polycythemia Vera; Primary Myelofibrosis; Transcobalamins; Vitamin B 12

Topics: Anemia, Pernicious; Animals; Biological Assay; Blood Proteins; Chickens; Cobalt Radioisotopes; Euglena gracilis; Female; Folic Acid Deficiency; Humans; Intrinsic Factor; Lactobacillus; Leukemia, Myeloid; Polycythemia Vera; Postgastrectomy Syndromes; Pregnancy; Protein Binding; Radioisotope Dilution Technique; Umbilical Cord; Vitamin B 12; Vitamin B 12 Deficiency

Topics: Age Factors; Aged; Female; Hematocrit; Hepatomegaly; Humans; Leukocytosis; Polycythemia Vera; Splenomegaly; Thrombocytosis; Vitamin B 12

Topics: Adult; Aged; Alkaline Phosphatase; Blood Platelets; Blood Volume; Bone Marrow; Chlorambucil; Erythrocyte Count; Erythropoietin; Female; Hemoglobins, Abnormal; Hookworm Infections; Humans; Hydrogen-Ion Concentration; Leukocyte Count; Male; Middle Aged; Oxygen; Phosphorus Radioisotopes; Polycythemia; Polycythemia Vera; Pruritus; Riboflavin Deficiency; Uric Acid; Vitamin B 12

Topics: Agranulocytosis; Ammonium Sulfate; Blood Proteins; Chemical Precipitation; Chromatography, DEAE-Cellulose; Chromatography, Gel; Cobalt Radioisotopes; Hematologic Diseases; Humans; Leukemia, Myeloid; Leukocytosis; Methods; Neutrophils; Polycythemia Vera; Protein Binding; Vitamin B 12; Vitamin B 12 Deficiency

Topics: Adolescent; Adult; Aged; Bilirubin; Blood Platelets; Bone Marrow; Bone Marrow Cells; Female; Folic Acid; Humans; Iron; Leukemia, Erythroblastic, Acute; Leukemia, Myeloid; Leukocytes; Male; Megaloblasts; Middle Aged; Polycythemia Vera; Reticulocytes; Transferrin; Vitamin B 12

Topics: Adult; Anemia, Pernicious; Chronic Disease; Female; Humans; Infant, Newborn; Leukemia, Myeloid; Male; Maternal-Fetal Exchange; Multiple Myeloma; Myeloproliferative Disorders; Placenta; Polycythemia Vera; Pregnancy; Primary Myelofibrosis; Vitamin B 12

Topics: Alpha-Globulins; Antigen-Antibody Reactions; Antigens; Beta-Globulins; Blood Proteins; Chromatography, DEAE-Cellulose; Chromatography, Gel; Cobalt Isotopes; Cross Reactions; Electrophoresis; Humans; Leukocytosis; Molecular Weight; Polycythemia Vera; Protein Binding; Reticulocytes; Vitamin B 12

Topics: Alpha-Globulins; Autoradiography; Beta-Globulins; Blood Proteins; Chromatography, DEAE-Cellulose; Cobalt Isotopes; Humans; Leukemia, Myeloid; Leukocytes; Polycythemia Vera; Primary Myelofibrosis; Protein Binding; Vitamin B 12

Topics: Anemia, Pernicious; Blood Proteins; Carrier Proteins; Centrifugation, Density Gradient; Child; Chromatography, Gel; Cobalt Isotopes; Electrophoresis; Fasting; Humans; Hydrogen-Ion Concentration; Isoelectric Focusing; Leukocytes; Molecular Weight; Polycythemia Vera; Protein Binding; Proteins; Remission, Spontaneous; Saliva; Tears; Vitamin B 12

Topics: Body Fluids; Electrophoresis; Humans; Hydrogen-Ion Concentration; Intrinsic Factor; Leukemia; Molecular Weight; Polycythemia Vera; Protein Binding; Vitamin B 12

Topics: Agranulocytosis; Anemia, Aplastic; Anemia, Macrocytic; Bone Marrow; Chronic Disease; Folic Acid; Humans; Leukemia, Myeloid; Leukocyte Count; Leukocytes; Muramidase; Myeloproliferative Disorders; Neutrophils; Polycythemia Vera; Protein Binding; Vitamin B 12

Topics: Agammaglobulinemia; Agranulocytosis; Anemia; Anemia, Hemolytic; Anemia, Hypochromic; Anemia, Macrocytic; Anemia, Sideroblastic; Antineoplastic Agents; Bone Marrow Diseases; Elliptocytosis, Hereditary; Female; Folic Acid; Folic Acid Deficiency; Humans; Iron; Myeloproliferative Disorders; Polycythemia Vera; Pregnancy; Primary Myelofibrosis; Spherocytosis, Hereditary; Thalassemia; Vitamin B 12; Vitamin B 12 Deficiency

Topics: Alkaline Phosphatase; Busulfan; Chlorambucil; Cyclophosphamide; Humans; Leukocyte Count; Muramidase; Phosphorus Isotopes; Polycythemia Vera; Uric Acid; Vitamin B 12

Topics: Aged; Anemia, Macrocytic; Calcium; Duodenal Ulcer; Erythropoietin; Hemoglobinometry; Humans; Iron; Leukocyte Count; Male; Phosphorus Isotopes; Polycythemia Vera; Postgastrectomy Syndromes; Uric Acid; Vitamin B 12

Topics: Anemia, Hemolytic; Anemia, Pernicious; Hodgkin Disease; Humans; Leukemia; Lymphoma, Non-Hodgkin; Malabsorption Syndromes; Polycythemia Vera; Primary Myelofibrosis; Radioisotope Dilution Technique; Schilling Test; Vitamin B 12

Topics: Aged; Anemia, Pernicious; Autoantibodies; Epistaxis; Fibrinolysis; Fluorescent Antibody Technique; Folic Acid; Globulins; Humans; Male; Melena; Phosphorus Isotopes; Polycythemia Vera; Radiotherapy; Reticulocytes; Vitamin B 12

Topics: Biological Transport; Cobalt Isotopes; Female; Fetus; HeLa Cells; Humans; In Vitro Techniques; Leukemia, Myeloid; Liver; Polycythemia Vera; Pregnancy; Protein Binding; Vitamin B 12

Topics: Adult; Aged; Blood Proteins; Bloodletting; Chlorambucil; Chromatography; Cyclophosphamide; Diagnosis, Differential; Hematocrit; Humans; Middle Aged; Myeloproliferative Disorders; Polycythemia; Polycythemia Vera; Protein Binding; Vitamin B 12

Topics: Aged; Antigen-Antibody Reactions; Binding Sites; Biological Assay; Blood Protein Electrophoresis; Bone Marrow Diseases; Chromatography; Chromatography, Gel; Cobalt Isotopes; Euglena; Glycoproteins; HeLa Cells; Humans; Male; Molecular Weight; Myeloproliferative Disorders; Phosphorus Isotopes; Polycythemia Vera; Vitamin B 12

Topics: Alpha-Globulins; Beta-Globulins; Cellulose; Charcoal; Cobalt Isotopes; Diagnosis, Differential; Humans; Leukemia, Myeloid; Leukocytosis; Methods; Myeloproliferative Disorders; Polycythemia Vera; Vitamin B 12

Topics: Alpha-Globulins; Beta-Globulins; Bone Marrow Diseases; Diagnosis, Differential; Female; Hematologic Diseases; Humans; Leukemia, Myeloid; Polycythemia Vera; Pregnancy; Pregnancy Complications, Hematologic; Primary Myelofibrosis; Prognosis; Protein Binding; Uremia; Vitamin B 12; Vitamin B 12 Deficiency

Topics: Adult; Alkaline Phosphatase; Anemia, Sideroblastic; Blood Cell Count; Blood Transfusion; Bone Marrow Examination; Diabetes Mellitus; Digestive System; Erythrocytes; Gastric Juice; Hematocrit; Hemoglobinometry; Hexachlorocyclohexane; Humans; Iron; Karyotyping; Liver; Male; Microscopy, Electron; Middle Aged; Mitochondria; Osmotic Fragility; Polycythemia Vera; Porphyrins; Radiography; Schilling Test; Thalassemia; Tryptophan; Vitamin B 12; Vitamin B 12 Deficiency

Topics: Adolescent; Adult; Aged; Alkaline Phosphatase; Bone Marrow Diseases; Chromosome Aberrations; Chromosome Disorders; Chromosomes, Human, 1-3; Chromosomes, Human, 13-15; Chromosomes, Human, 4-5; Chronic Disease; Diagnosis, Differential; Female; Hemorrhage; Hemostasis; Hepatomegaly; Humans; Leukemia, Myeloid; Leukocytes; Male; Megakaryocytes; Middle Aged; Myeloproliferative Disorders; Polycythemia Vera; Primary Myelofibrosis; Splenomegaly; Thrombocytosis; Vitamin B 12

Topics: Aged; Anemia, Pernicious; Autoantibodies; Body Height; Body Weight; Female; Fluorescent Antibody Technique; Gastric Acidity Determination; Gastric Juice; Gastric Mucosa; Gastritis; Histamine; Humans; Intrinsic Factor; Iron; Ischemia; Male; Middle Aged; Peptic Ulcer; Polycythemia Vera; Stomach Ulcer; Vitamin B 12

Topics: Anemia, Pernicious; Arteriosclerosis; Cobalt Isotopes; Computers; Humans; Multiple Sclerosis; Polycythemia Vera; Radiometry; Statistics as Topic; Time Factors; Vitamin B 12

Topics: Anemia, Pernicious; Antibodies; Female; Folic Acid; Gastric Mucosa; Hemoglobinometry; Humans; Intrinsic Factor; Male; Middle Aged; Polycythemia Vera; Vitamin B 12

Topics: Blood Proteins; Cellulose; Chromatography; Cobalt Isotopes; Humans; Polycythemia Vera; Protein Binding; Vitamin B 12

Topics: Adolescent; Adult; Anemia, Pernicious; Blood; Humans; In Vitro Techniques; Leukemia; Middle Aged; Polycythemia Vera; Vitamin B 12

Topics: Adolescent; Alkalies; Anemia; Anemia, Hypochromic; Anemia, Pernicious; Blood Chemical Analysis; Erythrocytes; Gastrectomy; Geriatrics; Hematologic Diseases; Humans; Lactobacillus; Leukemia; Leukemia, Lymphoid; Leukemia, Myeloid; Polycythemia Vera; Vitamin B 12; Vitamins

Topics: Anemia; Anemia, Pernicious; Corrinoids; Hematinics; Humans; Myocardial Infarction; Polycythemia; Polycythemia Vera; Toxicology; Vitamin B 12

Topics: Anemia; Anemia, Hypochromic; Anemia, Macrocytic; Anemia, Pernicious; Female; Folic Acid; Humans; Iron; Leukocytosis; Pelger-Huet Anomaly; Polycythemia Vera; Pregnancy; Pregnancy Complications; Pregnancy Complications, Hematologic; Thalassemia; Vitamin B 12

Topics: Anemia; Anemia, Macrocytic; Anemia, Megaloblastic; Drug Therapy; Humans; Polycythemia Vera; Vitamin B 12

Topics: Corrinoids; Humans; Polycythemia; Polycythemia Vera; Vitamin B 12

Topics: Biochemical Phenomena; Humans; Polycythemia Vera; Vitamin B 12; Vitamins

Topics: Bone Marrow; Bone Marrow Diseases; Hematologic Diseases; Hodgkin Disease; Humans; Leukemia; Myeloproliferative Disorders; Polycythemia Vera; Vitamin B 12

Topics: Anemia; Anemia, Hypochromic; Corrinoids; Hematologic Diseases; Humans; Polycythemia Vera; Vitamin B 12

Topics: Achlorhydria; Aged; Anemia; Ascorbic Acid; Hematologic Diseases; Humans; Iron; Polycythemia Vera; Vitamin B 12

Topics: Anemia; Anemia, Pernicious; Hematinics; Humans; Polycythemia; Polycythemia Vera; Vitamin B 12

Topics: Humans; Leukemia; Mercaptopurine; Polycythemia Vera; Vitamin B 12