vitamin-b-12 and Necrosis

Topics: Amino Acids; Anabolic Agents; Animals; Cardiac Glycosides; DNA; Drug Therapy, Combination; Folic Acid; Heart; Humans; Methylthiouracil; Myocardial Contraction; Myocardial Infarction; Myocardium; Necrosis; Orotic Acid; Protein Biosynthesis; Rabbits; RNA; Vitamin B 12

The aim of the study was to monitor the effect of butaphosphane (1-(n-butylamino)-1-methylophosphorous acid) and cyanocobalamin (Catosal preparation, Bayer AG) on regeneration of the longissimus lumborum muscle (musculus longissimus lumborum) in pigs. Experiments were conducted on 34 piglets of Polish Large White breed with a mean body weight of 20 kg that were divided into two groups. Piglets of group I (control) received an intramuscular injection of 10 cm3 of 0.5% bupivacaine hydrochloride at both side of the spine. Piglets of group II were injected with bupivacaine, as in group I, and additionally received intramuscular injections of 5 ml of Catosal for 5 subsequent days. The animals were euthanized 6, 12, and 24 hours as well as 2, 3, 4, 5, 7, 10 and 14 days after muscle injury. Preparations obtained from muscle specimens were stained with HE, PAS method acc. to McManus, HBFP, Feulgen, and Unna methods. Ultrastructural preparations (TEM) were prepared following a standard procedure. The presence of vimentin, desmin and PCNA was detected immunohistochemically in sections prepared with a paraffin method. Necrosis of muscle fibres was observed in all animals after bupivacaine injection. The administration of Catosal accelerated the regeneration of damaged skeletal muscles in pigs through the facilitation of phagocytosis and enhancement of myogenic cells proliferation. No effect of Catosal was found on differentiation of myoblasts or maturation of newly-formed muscle fibres.

Topics: Animals; Bupivacaine; Muscle Fibers, Skeletal; Muscular Diseases; Necrosis; Organophosphonates; Organophosphorus Compounds; Swine; Swine Diseases; Vitamin B 12

Parkinson's disease (PD) is a common disease whose pathophysiological mechanism is not well understood. Recent research studies have shown that PD patients have low serum levels of vitamins B12 and D. Therefore, in this study, the effects of supplementation with vitamins B12 and D on PD female mice as well as their fetuses were studied. After preparation of female mice and induction of Parkinson's by rotenone administration for 19 days, rotarod test was used to confirm PD induction. During this time, supplementations with vitamins B12 and D were performed. On day 19, after confirmation of PD induction, half of the mice were killed and the other half were allowed to mate with males. Viability was measured by the MTT method, and apoptosis and necrosis of cerebellar neurons were measured by flow cytometry. The RT-PCR technique was used to evaluate the relative expressions of the bax, bcl-2, miR-211, and circRNA 0,001,518 genes. Data analysis was performed by the GraphPad Prism V.8 software. Co-administration of vitamins B12 and D resulted in highest viability percentage and greatest reduction in apoptosis and necrosis of cerebellar neurons in the female mice as well as their fetuses compared to the PD females. A decrease in the relative expression of the bax and miR-211 genes and an increase in bcl-2 expression were observed in the cerebellar tissue of PD mice receiving both vitamins. Vitamins B12 and D have neuroprotective effects on PD conditions. Therefore, co-administration of these two vitamins is recommended in PD patients during pregnancy.

Topics: Animals; bcl-2-Associated X Protein; Female; Male; Mice; MicroRNAs; Necrosis; Neuroprotective Agents; Parkinson Disease; Pregnancy; Proto-Oncogene Proteins c-bcl-2; Vitamin B 12; Vitamins

Vitamin B12 is indispensable for proper brain functioning and cytosolic synthesis of S-adenosylmethionine. Whether its deficiency produces effects on viability and apoptosis of neurons remains unknown. There is a particular interest in investigating these effects in Parkinson disease where Levodopa treatment is known to increase the consumption of S-adenosylmethionine. To cause deprivation of vitamin B12, we have recently developed a cell model that produces decreased synthesis of S-adenosylmethionine by anchoring transcobalamin (TCII) to the reticulum through its fusion with Oleosin (OLEO).. Gene constructs including transcobalamin-oleosin (TCII-OLEO) and control constructs, green fluorescent protein-transcobalamin-oleosin (GFP-TCII-OLEO), oleosin-transcobalamin (OLEO-TCII), TCII and OLEO were used for expression in N1E-115 cells (mouse neuroblastoma) and in substantia nigra of adult rats, using a targeted transfection with a Neurotensin polyplex system. We studied the viability and the apoptosis in the transfected cells and targeted tissue. The turning behavior was evaluated in the rats transfected with the different plasmids.. The transfection of N1E-115 cells by the TCII-OLEO-expressing plasmid significantly affected cell viability and increased immunoreactivity of cleaved Caspase-3. No change in propidium iodide uptake (used as a necrosis marker) was observed. The transfected rats lost neurons immunoreactive to tyrosine hydroxylase. The expression of TCII-OLEO was observed in cells immunoreactive to tyrosine hydroxylase of the substantia nigra, with a superimposed expression of cleaved Caspase-3. These cellular and tissular effects were not observed with the control plasmids. Rats transfected with TCII-OLEO expressing plasmid presented with a significantly higher number of turns, compared with those transfected with the other plasmids.. In conclusion, the TCII-OLEO transfection was responsible for apoptosis in N1E-115 cells and rat substantia nigra and for Parkinson-like phenotype. This suggests evaluating whether vitamin B12 deficit could aggravate the PD in patients under Levodopa therapy by impairing S-adenosylmethionine synthesis in substantia nigra.

Topics: Animals; Apoptosis; Behavior, Animal; Cell Line, Tumor; Cell Survival; Humans; Intracellular Space; Methamphetamine; Mice; Necrosis; Parkinson Disease; Plant Proteins; Plasmids; Protein Transport; Rats; Recombinant Fusion Proteins; Substantia Nigra; Transcobalamins; Transfection; Transgenes; Vitamin B 12

Among the local complications following the intramuscular injection of therapeutic drugs, the syndrome of 'embolia cutis medicamentosa' deserves special attention. Shortly after the intramuscular injection, the patients notice severe pain and an erythematous discoloration of the skin at the injection site, followed by central demarcation and a sharply circumscribed skin necrosis with or without damage to peripheral nerves. We report about three patients, two of which had to be hospitalized. The drugs injected were combinations of analgesics of the phenylbutazone-type, corticosteroids, local anesthetics and cyanocobalamin. The observation that this severe complication may be associated with technically proper ventrogluteal injection of a wide array of therapeutic drugs shows that intramuscular injections require valid indications.

Topics: Aged; Aged, 80 and over; Female; Glucocorticoids; Humans; Injections, Intramuscular; Male; Middle Aged; Necrosis; Phenylbutazone; Skin; Vitamin B 12

Topics: Animal Feed; Animals; Erythrocytes; Kidney; Lipoproteins; Male; Necrosis; Organ Size; Rats; Rats, Inbred Strains; Vitamin B 12

Cardiovascular lesions were observed at necropsy in five of six sheep which were subjected to an experimentally induced cobalt-vitamin B12 deficiency during a period of 34 weeks. The classical symptoms of cobalt-vitamin B12 deficiency (anorexia, cachexia, anaemia) were seen in all six sheep at week 20 of the experiment. Histologically, lesions of the auricular epicardium and myocardium consisted of a high concentration of inflammatory cells dominated by polynuclear eosinophils as well as the haemorrhagic inflammation of blood vessels accompanied by necrosis of auricular tissue. The similarity between the lesions observed in the present experiment and those seen in arteriosclerosis, led to the suggestion that a deficiency of vitamin B12 may be implicated in the pathogenesis of arteriosclerosis. A hypothesis is advanced in this respect.

Topics: Animals; Arteriosclerosis; Cardiovascular Diseases; Cobalt; Myocardium; Necrosis; Sheep; Sheep Diseases; Vitamin B 12; Vitamin B 12 Deficiency

Twenty-one female patients studied between six and 12 months following a jejunoileal bypass procedure for obesity were found to have a wide variety of metabolic disturbances. Hepatic histological abnormalities were common and included liver cell necrosis and inflammation in nine patients and hepatic fibrosis in five. Liver function tests were no guide to the degree of hepatic impairment. Vitamin B12 malabsorption occurred in seven patients, in six probably as a result of bacterial intestinal overgrowth; three of these six patients had the most serious hepatic morphological changes. Malabsorption rather than poor oral intake of food appeared to account for continued postoperative weight loss in the majority of patients.

Topics: Adolescent; Adult; Female; Humans; Ileum; Jejunum; Liver; Liver Diseases; Malabsorption Syndromes; Middle Aged; Necrosis; Obesity; Vitamin B 12

Topics: Adolescent; Adult; Alkaline Phosphatase; Ascorbic Acid; Aspartate Aminotransferases; Bilirubin; Carotenoids; Cholesterol; Drainage; Female; Folic Acid; Glucose Tolerance Test; Hepatitis; Humans; Ileum; Intestinal Absorption; Intestinal Obstruction; Intestine, Small; Jejunum; Lipids; Male; Middle Aged; Necrosis; Obesity; Postoperative Complications; Surgical Wound Infection; Thrombophlebitis; Triglycerides; Uric Acid; Vitamin A; Vitamin B 12; Vitamin E; Xylose

Topics: Animals; Aspartate Aminotransferases; Chromatography, Gel; Culture Media; Dialysis; Liver; Molecular Weight; Myocardium; Necrosis; Rabbits; Streptococcus; Toxins, Biological; Trichloroacetic Acid; Vitamin B 12

Topics: Animals; Ascorbic Acid; Biotin; Carbon Tetrachloride Poisoning; Centrifugation; Chemical and Drug Induced Liver Injury; Folic Acid; Liver; Necrosis; Nicotinic Acids; Pantothenic Acid; Proteins; Pyridoxine; Rats; Riboflavin; Specimen Handling; Thiamine; Vitamin A; Vitamin B 12; Vitamin E; Vitamins

Topics: Animals; Cardiomyopathies; Chlorides; Disease Models, Animal; Electrocardiography; Immobilization; Magnesium; Methods; Myocardium; Necrosis; Potassium Chloride; Rabbits; Vitamin B 12

Topics: Avitaminosis; Carbon Tetrachloride Poisoning; Chemical and Drug Induced Liver Injury; DNA; Folic Acid; Folic Acid Deficiency; Hepatitis; Liver Diseases; Liver Regeneration; Metabolism; Necrosis; Pharmacology; Rats; Research; Thymidine; Uracil; Vitamin B 12

Topics: Anatomy; Androgens; Animals; Body Weight; Corrinoids; Cortisone; Incidence; Kidney Diseases; Liver Diseases; Mice; Myocardium; Necrosis; Pericarditis; Testosterone; Vitamin B 12

Topics: Carbon Tetrachloride; Cobalt; Corrinoids; Liver Diseases; Necrosis; Vitamin B 12