vitamin-b-12 and Leukocytosis

To evaluate the haematological changes induced by a long-distance race in well trained runners.. The haematological changes presented by 17 runners (15 men and 2 women) were assessed in a 6-hour race. For this purpose samples were examined before the race, 2 hours later, 4 hours later, at the end of the race, and 4 days after the trial.. Haemoglobin rates, as well as leucocyte, neutrophil, monocyte and platelet counts were increased, probably due to decreased plasma volume. Haemolytic traits were seen as well, namely, increased reticulocyte count and decreased haptoglobin. High transferrin-transport capability and serum B12 levels were also present. Mild haematuria was found without any haemosiderinuria, siderinuria or haemoglobinuria. All these changes recovered in the post-race study.. Changes in the major haematologic values are present during long-distance races, some of them due to haemoconcentration. Leucocytosis, haemolysis and haematuria are noteworthy, and these findings must be borne in mind when evaluating the health of runners.

Topics: Adult; Blood Cell Count; Blood Proteins; Female; Ferritins; Folic Acid; Hematuria; Hemoglobins; Hemolysis; Humans; Iron; Leukocytosis; Male; Middle Aged; Running; Transferrin; Vitamin B 12

A spontaneous oscillation of the white blood cell count was observed in a 58 year old man with chronic myelogenous leukemia (CML). Similar cyclic variations were noted in the platelet and reticulocyte counts with no apparent alterations in marrow cellularity to account for such changes. Since direct correlation was noted between white blood cells, platelets, and reticulocyte counts versus spleen size, it suggests that splenic hemopoiesis may be responsible for these cyclic changes. A possible inverse relationship between colony-stimulating factor (CSF) activity and the white blood cell count was noted, suggesting that CSF may be the humoral agent controlling granulocyte production. A direct correlation between the white blood cell count and serum unsaturated vitamin B12 binding capacity (UBBC) and lysozyme was also noted and further supports the concept that the latter two are measures of the granulocyte pool and metabolism. An inverse relationship between CSF activity and the UBBC suggests that these may be two different entities. Finally a modified form of standard chemotherapy may be effective in inducing remission in cases of CML with marked cyclic leukocytosis-leukopenia.

Topics: Alkaline Phosphatase; Blood Cell Count; Blood Platelets; Bone Marrow Examination; Colony-Stimulating Factors; Erythropoietin; Hemoglobins; Humans; Karyotyping; Leukemia, Myeloid; Leukocyte Count; Leukocytosis; Leukopenia; Male; Middle Aged; Muramidase; Periodicity; Reticulocytes; Spleen; Vitamin B 12

The unsaturated vitamin B12-binding capacities of the 'large molecular size vitamin B12-binding protein' (LBP) and the 'small molecular size vitamin B12-binding protein' (SBP) were determined by a Sephadex G 150 gel filtration method in 9 patients with chronic myelocytic leukaemia (CML), 5 patients with blast cell leukaemia and 12 patients with non-neoplastic leucocytosis. EDTA plasma and serum separated after 20 min and after 120 min were examined. In the 20 min EDTA plasma samples, the mean LBP value was 8,009 pg/ml in CML, 2,468 in blast leukaemia, 175 in non-neoplastic leucocytosis, and 57 in normal controls. The in vitro release of LBP into serum was much smaller in the leukaemias than in non-neoplastic leucocytosis. No correlation was found between the LBP values and the white blood cell counts or lysozyme values, but lysozyme was correlated to white cell count in CML. It is suggested that the plasma LBP levels reflect the fraction of LBP decay taking place at sites, e.g. the spleen, from which the released LBP can enter the circulation.

Topics: Adult; Aged; Carrier Proteins; Edetic Acid; Female; Granulocytes; Humans; Leukemia; Leukemia, Myeloid; Leukocytes; Leukocytosis; Male; Middle Aged; Muramidase; Protein Binding; Vitamin B 12

The R-type vitamin B12-binding protein (cobalophilin) in plasma and serum of patients with myeloproliferative diseases and leukocytosis was studied by gel filtration and isoelectric focusing. The isoproteins composing the cobalophilin were mainly the same in these disorders as in plasma of health subjects. In half the patients with chronic myelogenous leukaemia and a few of those with leukocytosis some of the isoproteins were more acid than those found in healthy subjects. In chronic myelogenous leukaemia the high unsaturated vitamin B12-binding capacity of plasma was due to an increase in the fairly acid isoproteins, whereas in polycythaemia vera and in most cases of leukocytosis there was an increase in the least acid isoproteins.

Topics: Blood Proteins; Humans; Isoelectric Focusing; Leukemia, Myeloid; Leukocytosis; Polycythemia Vera; Protein Binding; Vitamin B 12

A gradient was developed for isoelectric focusing in the pH range 2-5. Cobalophilin (earlier called R proteins or vitamin B12-binding proteins of R-type) was isolated from saliva and amniotic fluid in homogeneous form. It was found to be a glycoprotein with a molecular weight of 59,300-69,100. The preparation from amniotic fluid contained 33% carbohydrate. Cobalophilin variants in plasma, serum, granulocytes, platelets, amniotic fluid, milk, saliva and gastric juice were characterized by isoelectric focusing. Most fluids and cells contained the same isoproteins, with pI values between 2.3 and 5.0. Isoproteins of presumably myelogenic origin (e.g. those in granulocytes and plasma) had pI values below 4.2, whereas those of glandular origin (in milk and saliva) had a pI range of 4.0-5.0. Serum contained more cobalophilin than plasma, owing to release of this protein from granulocytes during clotting. This phenomenon also changed the isoprotein pattern. Plasma and serum from newborn infants and from patients with leucocytosis, polycythaemia vera and chronic myelogenous leukaemia contained the same isoproteins as were found in plasma from healthy subjects. In addition to these, isoproteins with lower than 'normal' pI values were often found in chronic myelogenous leukaemia and occasionally in leucocytosis. It is concluded that cobalophilin from different fluids and cells is a single microheterogeneous protein with a variable carbohydrate composition. The distribution of cobalophilin in different body fluids and cells supports the suggestion that cobalophilin is an antimicrobial protein (Gullberg 1972) like lactoferrin and lysozyme.

Topics: Amniotic Fluid; Blood Platelets; Blood Proteins; Gastric Juice; Glycoproteins; Granulocytes; Humans; Infant, Newborn; Leukemia, Myeloid; Leukocytosis; Milk, Human; Molecular Weight; Polycythemia Vera; Protein Binding; Saliva; Vitamin B 12

The unsaturated vitamin B12 binding capacity of whole serum (UBBC) and of the three transcobalamins (TC) has been studied in patients with various haematological diseases including myeloproliferative disorders (MPD) and acute leukaemia. The binding capacity of TC I and TC III was increased in MPD; TC I being particularly high in chronic granulocytic leukaemia (CGL) and TC III especially raised in polycythaemia rubra vera (PRV) and in infectious leucocytosis. The binding capacity of both TC I and TC III correlated with blood neutrophil count and the ratio TC III/TC I was low in CGL and increased in PRV. TC II was increased in acute myelogenous leukaemia, during remission and blast cell crisis of CGL and in refractory anaemia with excess of myeloblasts but not in acute lymphoblastic leukaemia (ALL). TC II correlated inversely with blood neutrophil count. There is an inverse ratio between TC II and TC I at least in myelogenous leukaemia. These abnormalities are discussed in relation to granulocyte kinetics. TC III and TC I reflect probably the total body granulocyte pool and share some biochemical and immunological properties supporting the view that they have a common origin in the more mature stages of the granulocyte cell line while TC II probably originates partly in more primitive granulocytes.

Topics: Anemia, Aplastic; Blood Cell Count; Blood Proteins; Humans; Leukemia; Leukemia, Lymphoid; Leukemia, Myeloid; Leukocyte Count; Leukocytosis; Myeloproliferative Disorders; Neutrophils; Polycythemia Vera; Primary Myelofibrosis; Transcobalamins; Vitamin B 12

Topics: Age Factors; Aged; Female; Hematocrit; Hepatomegaly; Humans; Leukocytosis; Polycythemia Vera; Splenomegaly; Thrombocytosis; Vitamin B 12

Topics: Aged; Alkaline Phosphatase; Diagnosis, Differential; Female; Humans; Leukemia, Myeloid; Leukocytes; Leukocytosis; Splenomegaly; Vitamin B 12

Topics: Agranulocytosis; Ammonium Sulfate; Blood Proteins; Chemical Precipitation; Chromatography, DEAE-Cellulose; Chromatography, Gel; Cobalt Radioisotopes; Hematologic Diseases; Humans; Leukemia, Myeloid; Leukocytosis; Methods; Neutrophils; Polycythemia Vera; Protein Binding; Vitamin B 12; Vitamin B 12 Deficiency

Topics: Anemia; Blood Cell Count; Blood Platelets; Blood Proteins; Colitis, Ulcerative; Crohn Disease; Hemoglobins; Humans; Hypoproteinemia; Intestine, Large; Intestine, Small; Iron; Leukocyte Count; Leukocytosis; Schilling Test; Thrombocytosis; Vitamin B 12; Vitamin B 12 Deficiency

Topics: Agranulocytosis; Blood Cell Count; Cell Division; Humans; In Vitro Techniques; Leukocyte Count; Leukocytes; Leukocytosis; Lithium; Neutrophils; Vitamin B 12

Topics: Alkaline Phosphatase; Blood Viscosity; Bone Marrow Examination; Carrier Proteins; Erythropoietin; Female; Hematocrit; Hemoglobinopathies; Histamine; Humans; Hypoxia; Leukocytosis; Male; Plasma Volume; Polycythemia; Splenomegaly; Thrombocytosis; Uric Acid; Vitamin B 12

Topics: Adult; Aged; Alpha-Globulins; Beta-Globulins; Blood Proteins; Child, Preschool; Chronic Disease; Female; Fever of Unknown Origin; Hodgkin Disease; Humans; Infections; Leukocyte Count; Leukocytosis; Liver Neoplasms; Lymphoma; Male; Middle Aged; Neoplasm Metastasis; Neoplasms; Protein Binding; Splenectomy; Vitamin B 12

Topics: Alpha-Globulins; Antigen-Antibody Reactions; Antigens; Beta-Globulins; Blood Proteins; Chromatography, DEAE-Cellulose; Chromatography, Gel; Cobalt Isotopes; Cross Reactions; Electrophoresis; Humans; Leukocytosis; Molecular Weight; Polycythemia Vera; Protein Binding; Reticulocytes; Vitamin B 12

Topics: Adult; Alpha-Globulins; Beta-Globulins; Chromatography, DEAE-Cellulose; Cobalt Isotopes; Diagnosis, Differential; Electrophoresis, Disc; Female; Humans; Leukemia, Myeloid; Leukemoid Reaction; Leukocyte Count; Leukocytosis; Male; Middle Aged; Neutrophils; Protein Binding; Vitamin B 12

Topics: Alpha-Globulins; Beta-Globulins; Cellulose; Charcoal; Cobalt Isotopes; Diagnosis, Differential; Humans; Leukemia, Myeloid; Leukocytosis; Methods; Myeloproliferative Disorders; Polycythemia Vera; Vitamin B 12

Topics: Anemia, Hemolytic, Autoimmune; Basophils; Blood Platelet Disorders; Bone Marrow; Chromosome Aberrations; Chromosome Disorders; Diagnosis, Differential; Female; Humans; Karyotyping; Leukemia, Myeloid; Leukocytosis; Middle Aged; Primary Myelofibrosis; Vitamin B 12

Topics: Adolescent; Adult; Anemia, Aplastic; Anemia, Sickle Cell; Bilirubin; Electrophoresis; Female; FIGLU Test; Folic Acid Deficiency; Hemoglobinometry; Histidine; Humans; Leukocytosis; Paper; Starch; Thrombocytopenia; Vitamin B 12

Topics: Anemia; Anemia, Hypochromic; Anemia, Macrocytic; Anemia, Pernicious; Female; Folic Acid; Humans; Iron; Leukocytosis; Pelger-Huet Anomaly; Polycythemia Vera; Pregnancy; Pregnancy Complications; Pregnancy Complications, Hematologic; Thalassemia; Vitamin B 12