vitamin-b-12 and Laryngeal-Neoplasms

We have shown that hydroxycobalamin (vitamin В

Topics: Apoptosis; Autophagy; Carcinoma; Cell Death; Cell Line, Tumor; Cell Survival; Ditiocarb; Drug Synergism; Endoplasmic Reticulum; Endoplasmic Reticulum Stress; Humans; Hydroxocobalamin; Laryngeal Neoplasms; Larynx; Mitochondria; Oxidative Stress; Vacuoles; Vitamin B 12; Vitamins

Thrombotic thrombocytopenic purpura (TTP) is a thrombotic microangiopathy with neurological disorder which incidence is relatively rare. Since the discovery of the ADAMTS 13 in 1996 and the changes in the treatment (plasma exchanges and rituximab), the prognosis of the TTP has considerably improved. We report the case of a 60-year-old man with a refractory TTP. The subsequent discovery of an epiglottis neoplasia and its treatment saved the patient.

Topics: Adrenal Cortex Hormones; Antibodies, Monoclonal, Murine-Derived; Antimetabolites, Antineoplastic; Antineoplastic Agents; Antineoplastic Agents, Phytogenic; Antineoplastic Combined Chemotherapy Protocols; Carcinoma, Squamous Cell; Cisplatin; Docetaxel; Epiglottis; Fluorouracil; Humans; Laryngeal Neoplasms; Male; Middle Aged; Plasma Exchange; Plasmapheresis; Platelet Count; Purpura, Thrombotic Thrombocytopenic; Rituximab; Taxoids; Vitamin B 12; Vitamins

Metabolic changes in head and neck carcinogenesis are often non-specifically correlated with carcinomas. The study of metabolic disorders can improve the understanding of tumourigenesis at the cellular level. This study was designed to evaluate the role of serum homocysteine, folate, and vitamin B(12) levels in the pathogenesis of laryngeal squamous cell cancer (LSCC) by measuring serum levels in 60 consecutive untreated patients with LSCC and 60 controls (30 smokers and 30 non-smokers). Compared with smoker and non-smoker control groups, significantly lower levels of vitamin B(12) were found in patients with LSCC. Folate levels in patients with LSCC were also significantly lower than in the smoker and non-smoker control groups. There were no significant differences in the homocysteine levels between these three groups. Metabolic alterations in vitamin B(12) and folate levels, particularly hypofolataemia, could be associated with the development of LSCC, although further research is required to confirm their roles definitively.

Topics: Carcinoma, Squamous Cell; Case-Control Studies; Demography; Folic Acid; Homocysteine; Humans; Laryngeal Neoplasms; Middle Aged; Vitamin B 12

To determine plasma levels of homocysteine, folate, and vitamin B12 in patients with laryngeal cancer and a control group.. Analysis of homocysteine, folate, and vitamin B12 levels in 25 consecutive untreated patients with laryngeal carcinoma and 80 healthy control participants. The study and control groups were subdivided into smokers, ex-smokers, and nonsmokers, as well as drinkers and nondrinkers.. The AxSYM system was used to measure total homocysteine levels, and the ARCHITECT system (both Abbott-Diagnostics Division) was used to measure folate and vitamin B12 levels.. Homocysteine, folate, and vitamin B12 levels.. The mean (SD) level of total homocysteine in patients with laryngeal carcinoma was 2.84 (1.62) mg/L vs 0.99 (0.24) mg/L in the control group (P <.001). The mean (SD) folate plasma level was 4.3 (2.2) ng/mL vs 7.9 (2.4) ng/mL (P <.001).. Metabolic alterations in homocysteine, folate, and vitamin B12 levels, especially hypofolatemia, could be associated with laryngeal cancer. Lengthier follow-up studies and larger groups of patients will help determine the real role of these metabolic alterations.

Topics: Adult; Aged; Aged, 80 and over; Female; Folic Acid; Homocysteine; Humans; Laryngeal Neoplasms; Male; Middle Aged; Vitamin B 12

The authors evaluated serum levels of folate, homocysteine, and vitamin B(12) in patients with head and neck squamous cell carcinoma (HNSCC) and in patients with laryngeal leukoplakia, a well known preneoplastic lesion.. One hundred forty-four consecutive, untreated patients with HNSCC and 40 consecutive, untreated patients with laryngeal leukoplakia were enrolled in the Department of Otolaryngology at the authors' institution. Data from those patients were compared with data from one control group, which included 90 smokers, and from another control group, which included 120 nonsmokers. Serum levels of homocysteine, folate, and vitamin B(12) were measured by an automated immunoassay method based on fluorescence polarization immunoassay technology.. Comparing groups by Student-Newman-Keuls test, serum folate levels were significantly lower in patients with HNSCC and in patients with laryngeal leukoplakia compared with serum folate levels in both the smoker control group and the nonsmoker control group. Serum homocysteine levels in patients with HNSCC were significantly higher compared with homocysteine levels both in the smoker and nonsmoker control groups and in patients with laryngeal leukoplakia. There were no statistically significant differences between groups in serum vitamin B(12) levels.. A role for folate deficiency as a risk factor in head and neck carcinogenesis is plausible. A chemoprevention protocol with folate is both feasible and ethically correct and is in progress at the authors' institution. Homocysteine levels in patients with HNSCC probably are affected largely by the HNSCC phenotype. An accumulation of homocysteine may reveal a genetic defect, which, theoretically, may be a target for pharmacologic therapy, for example, with antifolic drugs.

Topics: Adult; Aged; Aged, 80 and over; Analysis of Variance; Biomarkers, Tumor; Carcinoma, Squamous Cell; Case-Control Studies; Female; Folic Acid; Head and Neck Neoplasms; Homocysteine; Humans; Laryngeal Neoplasms; Leukoplakia; Male; Middle Aged; Prognosis; Reference Values; Risk Assessment; Sensitivity and Specificity; Vitamin B 12

Incubation of human laryngeal epidermoid carcinoma HEp-2 cells with hydroxocobalamin (vitamin B12b) and ascorbic acid (vitamin C) for 1 h initiated oxidative stress accompanied by damage to mitochondria and increase in intracellular oxidative activity. Studies of the kinetics of these processes showed that the increase in intracellular H2O2 activity and mitochondrial damage are more likely a result, but not the cause of cell apoptosis during the first hour of their incubation with vitamins B12b and C.

Topics: Ascorbic Acid; Calcium; Cell Death; Cell Line, Tumor; Fluoresceins; Fluorescent Dyes; Humans; Hydrogen Peroxide; Hydroxocobalamin; Kinetics; Laryngeal Neoplasms; Membrane Potentials; Mitochondria; Oxidative Stress; Spectrometry, Fluorescence; Time Factors; Vitamin B 12

The combination of hydroxocobalamin (vitamin B12b) and ascorbic acid (vitamin C) can cause the death of tumor cells at the concentrations of the components at which they are nontoxic when administered separately. This cytotoxic action on epidermoid human larynx carcinoma cells HEp-2 in vitro is shown to be due to the hydrogen peroxide generated by the combination of vitamins B12b and C. The drop in the glutathione level preceding cell death was found to be the result of combined action of the vitamins. It is supposed that the induction of cell death by combined action of vitamins B12b and C is connected to the damage of the cell redox system.

Topics: Ascorbic Acid; Carcinoma, Squamous Cell; Cell Division; Drug Synergism; Glutathione; Humans; Hydrogen Peroxide; Laryngeal Neoplasms; Oxidation-Reduction; Tumor Cells, Cultured; Vitamin B 12

Twenty-two cases of early squamous cell carcinoma of the arytenoid cartilage staged as T1 according to the 1983 American Joint Committee for Cancer Staging Classification system were reviewed. Eighteen percent of the patients showed no symptoms. In the remaining 82%, the main presenting symptom was pain in the form of sore throat, odynophagia, or otalgia. Radiotherapy and partial laryngeal surgery were the options retained for the treatment of the larynx. A "watch and wait" policy or preventive treatment of the ipsilateral jugulocarotid lymph nodes were the treatment options retained for the neck. Significant differences were noted in terms of local and nodal recurrence between the two programs. Results indicate that partial laryngeal surgery with total arytenoidectomy and preventive treatment of the neck appear to be advisable for this particular type of primary lesion.

Topics: Adult; Aged; Aged, 80 and over; Antineoplastic Combined Chemotherapy Protocols; Arytenoid Cartilage; Carcinoma, Squamous Cell; Cisplatin; Cobalt Radioisotopes; Female; Fluorouracil; Follow-Up Studies; Head and Neck Neoplasms; Humans; Laryngeal Neoplasms; Laryngectomy; Lymphatic Metastasis; Male; Middle Aged; Neoplasm Recurrence, Local; Neoplasm Staging; Radiotherapy Dosage; Radiotherapy, High-Energy; Survival Rate; Treatment Outcome; Vitamin B 12

Topics: Animals; Carbon Isotopes; Carcinoma; Cell Division; Cell Line; Cricetinae; Culture Media; Culture Techniques; Drug Synergism; Folic Acid; Formaldehyde; HeLa Cells; Homocysteine; Humans; Kidney; Laryngeal Neoplasms; Methionine; Stimulation, Chemical; Swine; Transferases; Vitamin B 12

Topics: Carbon Isotopes; Carcinoma, Squamous Cell; Culture Techniques; Homocysteine; Humans; Laryngeal Neoplasms; Ligases; Methionine; Quaternary Ammonium Compounds; Radioisotopes; Sulfates; Vitamin B 12

Topics: Corrinoids; Humans; Laryngeal Neoplasms; Maxillary Neoplasms; Neoplasms; Pathology; Surgical Procedures, Operative; Vitamin B 12

Topics: Adenine; Adrenochrome; Blood Transfusion; Corrinoids; Humans; Laryngeal Neoplasms; Leukocyte Count; Maxillary Neoplasms; Nose Neoplasms; Radiotherapy; Tongue Neoplasms; Vitamin B 12