vitamin-b-12 and Infertility--Female

Topics: Air Pollution, Indoor; Anesthesia, Dental; Anesthetics, Inhalation; Animals; Bone Marrow; Bone Marrow Diseases; Dental Hygienists; Dental Staff; Female; Gas Scavengers; Humans; Infertility, Female; Male; Maximum Allowable Concentration; National Institute for Occupational Safety and Health, U.S.; Nitrous Oxide; Occupational Exposure; Pregnancy; Respiratory Protective Devices; United States; United States Occupational Safety and Health Administration; Vitamin B 12

This contribution deals with the occurrence of cyanide and its biological pathways in the body. Especially possibilities of detoxification are pointed out. Intoxications are caused by acute and chronical cyanide uptake. Tobacco amblyopia, retrobulbar neuritis in pernicious anaemia, Leber's optic atrophy, Nigerian nutritional neuropathy, and sterility in female heavy smokers are attributed to cyanide intoxication. Various methods for treating acute and chronic cyanide intoxication are discussed.

Topics: Acute Disease; Amblyopia; Anemia, Pernicious; Animals; Antidotes; Chronic Disease; Cyanides; Female; Humans; Inactivation, Metabolic; Infertility, Female; Manihot; Nigeria; Optic Atrophy; Optic Neuritis; Plants, Edible; Scotoma; Smoking; Syndrome; Thiocyanates; Thiosulfate Sulfurtransferase; Vitamin B 12

The interplay between female fertility and autoimmune diseases (AIDs) can involve HLA haplotypes and micronutrients. We analyzed the distribution of HLA-DQ2/-DQ8 in women with infertility or recurrent spontaneous abortion (RSA) and possible associations with AIDs and micronutrient status.. Consecutive women (. DQA1*05/B1*02 and the occurrence of at least one DQ2 allele were more prevalent among RSA and infertile women than controls. Infertile women showed lower 25(OH)D and higher prevalence of AIDs than RSA women. In the multivariate analysis, DQA1*05/B1*02 was associated with a significantly higher risk of AIDs in infertile women, and DQA1*05 was independently associated with both 25(OH)D deficiency and AIDs. In RSA women, the presence of AIDs was associated with a significantly higher risk of 25(OH)D deficiency.. Our findings showed, for the first time, a higher proportion of DQ2 alleles in infertile and RSA women as compared to controls. Predisposing DQ2 alleles are independent risk factors for AIDs and 25(OH)D deficiency in infertile women and could represent biomarkers for performing early detection of women requiring individually tailored management.

Topics: Abortion, Habitual; Adult; Alleles; Autoimmune Diseases; Autoimmunity; Biomarkers; Female; Ferritins; Folic Acid; Genetic Predisposition to Disease; Genotype; Haplotypes; HLA-DQ Antigens; Humans; Infertility, Female; Micronutrients; Nutritional Status; Pregnancy; Risk Factors; Vitamin B 12; Vitamin D; Vitamin D Deficiency

The homocysteine pathway in the preconception period should be evaluated to highlight micronutrient deficiencies and warrants optimal multivitamin supplementation, before Assisted Reproduction, as preconception care. We conducted a retrospective study aimed at investigating the role of vitamin B complex (5-methyltetrahydrofolate, vitamin B12, vitamin B6) supplement use compared with the role of only folic acid supplement use, in relation to clinical pregnancy and live birth in infertile women undergoing homologous ART. We investigated 269 Caucasian women referred to the Centre for Assisted Reproductive Technology for homologous ART. In these women, 111 (Group A) were daily supplemented with vitamin B complex and 158 (Group B) with only folic acid. In group A the mean number of Metaphase II oocytes and the 2PN Fertilization Rate were higher in comparison to group A (

Topics: Dietary Supplements; Female; Folic Acid; Homocysteine; Humans; Infertility, Female; Live Birth; Pregnancy; Reproductive Techniques, Assisted; Retrospective Studies; Tetrahydrofolates; Vitamin B 12; Vitamin B Complex

The purpose of this study was to evaluate the vitamin B status related to the homocysteine pathway and the prevalence of polymorphisms of the MTHFR gene in infertile women programming homologous or heterologous ART.. We investigated 393 consecutive Caucasian women, referred to the Internal Medicine Clinic at the Center for Assisted Reproductive Technology, in order to be framed for their vascular risk before starting homologous or heterologous (oocyte donation) procedures. Total homocysteine, Vitamin B12, folate and vitamin B6 were measured. The women were divided into quartiles of serum concentration of folate, vitamin B12 and vitamin B6. The C677T and A1298C polymorphisms of the MTHFR gene were genotyped by an electronic microchip technology.. Sixty-one women (15.5%) had hyperhomocysteinemia, 22.9% had reduced levels of vitamin B12, 4.1% had reduced levels of serum folate and 0.1% had a deficiency of vitamin B6. Women in the highest quartile of vitamin B12 and folates had lower homocysteine ​​levels than women in the first and second quartiles (p < 0.0001). The homozygosity for MTHFR C677T polymorphism was detected in 33.3% (131), and heterozygosity for MTHFR C677T polymorphism in 45.3% (178) of women. We observed a significant association between hyperhomocysteinemia and 677T allele, but not 1298C, of the MTHFR polymorphisms (p = 0.04).. We found inadequate vitamin B status related to the homocysteine ​​pathway in women planning Assisted Reproductive Technology. Moreover, interesting association was found regarding hyperhomocysteinemia in women carrying T allele of the C677T MTHFR polymorphism. A specific supplementation with 5-MTHF and adequate vitamin B12 concentrations before Assisted Reproductive Technology warrant serious consideration, in particular in women carrying T allele of the C677T MTHFR polymorphism.

Topics: Female; Folic Acid; Genotype; Homocysteine; Humans; Infertility, Female; Italy; Methylenetetrahydrofolate Reductase (NADPH2); Vitamin B 12; Vitamin B Complex

Topics: Abortion, Habitual; Adult; Autoimmune Diseases; Female; Gastritis, Atrophic; Humans; Infertility, Female; Pregnancy; Pregnancy Outcome; Vitamin B 12

Italian public health authorities recommend women of childbearing age to assume a daily dose of 0.4 mg of folic acid (FA) from at least one month before conception in order to reduce the risk of having children affected by neural tube defects (NTDs). In this study, folate, homocysteine and vitamin B12 serum levels were determined in 77 women entering an in vitro fertilization program. About 75% of patients had serum folate values compatible with the intake of the recommended dose of FA for at least three months, whereas only the 61% of them reached or exceeded the serum folate concentration regarded as the optimal concentration during the periconceptional period. Mean vitamin B12 serum levels and mean homocysteine plasma levels resulted in normal range in all the women with mean values of 381.2 ± 2.2 pg/ml and 8.48 ± 2.2 μmol/l, respectively. In conclusion, only a portion of women entering an IVF program presents proper folate levels.

Topics: Adult; Female; Fertilization in Vitro; Folic Acid; Homocysteine; Humans; Infertility, Female; Vitamin B 12

Diet has been recognised as a crucial factor influencing fetal and maternal health. Adequate levels of substances such as homocysteine, folate and vitamin B. Fasting venous blood samples were taken in the morning during routine screening before IVF in order to measure: serum folate, red blood cell (RBC) folate, total plasma homocysteine, vitamin B. Among 269 women aged 37 ± 4 years, only 69% and 44% showed adequate levels of homocysteine and vitamin B. Folate levels were largely inadequate among women attending an infertility clinic for IVF. Vitamin B

Topics: Adult; Cross-Sectional Studies; Female; Ferritins; Folic Acid; Homocysteine; Humans; Infertility, Female; Iron; Trace Elements; Vitamin A; Vitamin B 12; Vitamin B Complex; Vitamin E

To investigate associations between folate-metabolizing gene variations, folate status, and unexplained female infertility.. An association study.. Hospital-based IVF unit and university-affiliated reproductive research laboratories.. Seventy-one female patients with unexplained infertility.. Blood samples for polymorphism genotyping and homocysteine, vitamin B12, and folate measurements.. Allele and genotype frequencies of the following polymorphisms: 5,10-methylenetetrahydrofolate reductase (MTHFR) 677C/T, 1298A/C, and 1793G/A, folate receptor 1 (FOLR1) 1314G/A, 1816delC, 1841G/A, and 1928C/T, transcobalamin II (TCN2) 776C/G, cystathionase (CTH) 1208G/T and solute carrier family 19, member 1 (SLC19A1) 80G/A, and concentrations of plasma homocysteine, vitamin B12, and serum folate.. MTHFR genotypes 677CT and 1793GA, as well as 1793 allele A were significantly more frequent among controls than in patients. The common MTHFR wild-type haplotype (677, 1298, 1793) CAG was less prevalent, whereas the rare haplotype CCA was more frequent in the general population than among infertility patients. The frequency of SLC19A1 80G/A genotypes differed significantly between controls and patients and the A allele was more common in the general population than in infertile women. Plasma homocysteine concentrations were influenced by CTH 1208G/T polymorphism among infertile women.. Polymorphisms in folate pathway genes could be one reason for fertility complications in some women with unexplained infertility.

Topics: Adult; Carrier Proteins; Cross-Sectional Studies; Cystathionine gamma-Lyase; Female; Folate Receptor 1; Folate Receptors, GPI-Anchored; Folic Acid; Genetic Association Studies; Genetic Variation; Homocysteine; Humans; Infertility, Female; Membrane Transport Proteins; Methylenetetrahydrofolate Reductase (NADPH2); Polymorphism, Genetic; Receptors, Cell Surface; Reduced Folate Carrier Protein; Signal Transduction; Transcobalamins; Vitamin B 12

A high prevalence of low levels of cobalamin had been found in a survey of multi-ethnic normal individuals in Israel. The purpose of this study was to investigate the incidence of cobalamin deficiency among Israeli couples suffering from infertility. All couples seen at the in vitro fertilization clinic at an urban hospital (Shaare Zedek Medical Center) in Jerusalem for a 6-month period were invited. Mean cobalamin levels were 259.2 pg ml(-1) in males and 275.1 pg ml(-1) in females (normal >200 pg ml(-1)), 35.5% of 172 men and 23.3% of 223 females had cobalamin deficiency (P = 0.01). There were 171 couples with complete demographic questionnaires and cobalamin values for each partner. In 74 couples (43.3%), one partner was cobalamin deficient, with no significant difference between those with unexplained infertility versus those with explained infertility; and in 13 couples, both partners were cobalamin deficient. Thirty-nine per cent of all men with an abnormal semen analysis had cobalamin deficiency, a finding that requires further investigation. This study questions whether higher rates of male infertility in Israel are partially ascribable to cobalamin deficiency. Recommendation for supplementation in both males and females to achieve high-normal levels of cobalamin would be prudent.

Topics: Adult; Female; Humans; Infertility, Female; Infertility, Male; Israel; Male; Middle Aged; Prevalence; Vitamin B 12; Vitamin B 12 Deficiency

A poor quality of oocytes and embryos and a low fertilization rate have been found in polycystic ovary syndrome (PCOS) patients. An inverse association between follicular fluid homocysteine (Hcy) levels and oocyte and embryo quality has also been demonstrated. We examined the relationship between follicular fluid Hcy concentrations and oocyte and embryo quality in PCOS patients undergoing assisted reproduction.. Fifty-two PCOS patients were included in the study, and underwent GnRH agonist/recombinant FSH treatment. The Hcy, folate, vitamin B(12), malonyldialdehyde (MDA) and estradiol (E(2)) levels were measured in follicular fluid from single oocytes at time of retrieval. One follicle per ovary was sampled and 94 were analysed. Plasma hormones were also measured. Oocytes and embryos were graded (1-3) using standard approaches.. The concentrations of Hcy, E(2), vitamin B(12), folate and MDA in plasma were higher than in follicular fluid (all P < 0.001). Significant differences were observed in follicular Hcy levels between Grade 3 and Grade 2 oocytes (P < 0.001). Hcy levels were lower in Grade 1-2 embryos than that in Grade 3 embryos; follicular fluid vitamin B(12) levels were lower in patients showing high concentrations of follicular fluid Hcy (P < 0.01). The follicular fluid Hcy levels were negatively correlated with follicular fluid vitamin B(12) (r = -0.44), folate (r = -0.68) and fertilization rate (r = -0.85), and positively correlated with follicular fluid MDA (r = 0.51).. Concentrations of Hcy in follicular fluid on the dOPU may be a useful marker for fertilization rate, and oocyte and embryo quality in PCOS patients undergoing assisted reproduction.

Topics: Adult; Embryo, Mammalian; Estradiol; Female; Folic Acid; Follicle Stimulating Hormone; Follicular Fluid; Homocysteine; Humans; Infertility, Female; Malondialdehyde; Oocytes; Ovulation Induction; Polycystic Ovary Syndrome; Reproductive Techniques, Assisted; Vitamin B 12

Steroid hormones including oestradiol have been identified as non-genetic factors that may influence plasma homocysteine. In advanced assisted reproduction, plasma oestradiol levels fluctuate markedly during the treatment cycle starting with normal followed by sub- and then supra-physiological levels. Because of the diverse harmful effects that hyperhomocysteinaemia has been associated with, it is imperative to understand how it may be manipulated during assisted reproduction. A total of 30 women undergoing treatment for infertility were recruited and followed through an advanced assisted reproduction cycle. Blood samples were analysed for oestradiol, homocysteine, vitamin B12, red cell folate and plasma folate during each phase. All patients had normal vitamin B12 and folate levels. Predictably, oestradiol showed marked changes as patients progressed through each treatment phase. However, there were no corresponding significant fluctuations in plasma homocysteine. Oestradiol concentration does not influence plasma homocysteine in women with normal vitamin B12 and folate levels undergoing advanced assisted reproduction.

Topics: Adult; Estradiol; Female; Folic Acid; Homocysteine; Humans; Infertility, Female; Reproductive Techniques, Assisted; Vitamin B 12

Chronic exposure to ambient nitrous oxide levels found in dental offices may pose unrecognized health risks, among them, increased incidence of spontaneous abortion or infertility among female workers. Vitamin B12 supplements can reverse some of the effects of chronic N2O exposure.

Topics: Abortion, Spontaneous; Dental Staff; Female; Humans; Infertility, Female; Nitrous Oxide; Occupational Diseases; Pregnancy; Vitamin B 12

After starting treatment with vitamin B12, a 32-year-old woman with pernicious anaemia and secondary infertility became pregnant again and gave birth to a healthy child. It has been found that pernicious anaemia, which is rare during childbearing age, usually leads to infertility in men and women. This is reversible by treatment with vitamin B12.

Topics: Anemia, Pernicious; Body Weight; Female; Humans; Infertility, Female; Pregnancy; Vitamin B 12

Topics: Adolescent; Adult; Anemia, Pernicious; Female; Humans; Infertility, Female; Pregnancy; Vitamin B 12

Topics: Animals; Aspartate Aminotransferases; Blood Glucose; Cattle; Cholesterol; Female; Fertility; Infertility, Female; Organophosphonates; Pregnancy; Pregnancy, Animal; Uterine Diseases; Vitamin B 12

Topics: Animals; Cobalt; Female; Infertility, Female; Liver; Plant Poisoning; Selenium; Sheep; Sheep Diseases; Vitamin B 12

Topics: Adolescent; Adult; Anemia, Pernicious; Female; Folic Acid; Humans; Infertility, Female; Pregnancy; Pregnancy Complications, Hematologic; Vitamin B 12; Vitamin B 12 Deficiency

Topics: Cervix Uteri; Disease; Female; Humans; Infertility; Infertility, Female; Uterine Diseases; Vitamin B 12