vitamin-b-12 and Colitis

Background Folate is an important vitamin with protective effect against some human diseases. The aim of this study was to evaluate the relationship between serum folate levels, inflammatory markers and disease clinical activity in patients with inflammatory bowel disease (IBD).   Methods The participants were classified into two groups in which 38 IBD patients and 38 healthy controls were studied. Disease clinical activities were evaluated by means of established score systems. Serum folate, homocysteine and C-reactive protein and ESR were measured. Obtained data were analyzed with proper statistical methods and P- value less than 0.05 was considered as statistical significant.   Results The level of serum folate was significantly reduced in IBD patients with active disease compared to patients with clinical remission (p=0.043) and also healthy controls (p = 0.008). Moreover, there was a significant inverse correlation between serum folate levels and C-reactive protein in IBD patients (r = -0.563 p =0.001).         Conclusion Serum folate levels is associated with inflammatory markers and disease clinical activity in IBD patients, therefore there is a possibility that disease clinical activity is reduced with adequate folate level.

Topics: Colitis; Folic Acid; Homocysteine; Humans; Inflammatory Bowel Diseases; Vitamin B 12

Increased delivery of taurine-conjugated bile acids to the distal bowel can lead to dysbiosis resulting in colitis in mouse models of inflammatory bowel disease. A similar situation also could occur in cats with intestinal disease and might therefore result in decreased whole-body taurine concentration.. To determine whether whole-blood taurine concentrations are decreased at the time of diagnosis in cats with intestinal disease and to correlate concentrations with clinical and laboratory variables.. Twenty-one cats with chronic inflammatory enteropathy and 7 cats with intestinal neoplasia from the University of Bristol.. Cats that had undergone a thorough investigation consisting of a CBC, serum biochemistry, serum cobalamin and folate concentrations, transabdominal ultrasound examination and histopathology of intestinal biopsy specimens, as well as additional testing if indicated, were included. Whole-blood from these cats collected at the time of histologic diagnosis and stored in ethylenediaminetetraacetic acid was retrospectively analyzed for taurine with an automated high-performance liquid chromatography amino acid analyzer.. Although whole-blood taurine concentrations remained within the reference range, those cats with predominantly large intestinal clinical signs had significantly lower concentrations than did cats with small intestinal and mixed bowel clinical signs (P = 0.033) and this difference also was significant when assessed only in cats with chronic inflammatory enteropathy (P = 0.019).. Additional studies are needed to determine whether large intestinal signs in cats with chronic inflammatory enteropathy are caused by alterations in the microbiota arising as a consequence of increased delivery of taurine-conjugated bile acids.

Topics: Animals; Cat Diseases; Cats; Chromatography, High Pressure Liquid; Colitis; Female; Folic Acid; Intestinal Diseases; Intestinal Neoplasms; Male; Serum Albumin; Taurine; Vitamin B 12

Developmental epigenetic changes, such as DNA methylation, have been recognized as potential pathogenic factors in inflammatory bowel diseases, the hallmark of which is an exaggerated immune response against luminal microbes. A methyl-donor (MD) diet can modify DNA methylation at select murine genomic loci during early development. The components of the MDs are routinely incorporated into prenatal human supplements. Therefore, we studied the effects of maternal MD supplementation on offspring colitis susceptibility and colonic mucosal DNA methylation and gene expression changes in mice as a model. Additionally, we investigated the offspring mucosal microbiomic response to the maternal dietary supplementation. Colitis was induced by dextran sulfate sodium. Colonic mucosa from offspring of MD-supplemented mothers following reversal to control diet at weaning was interrogated by methylation-specific microarrays and pyrosequencing at postnatal days 30 (P30) and P90. Transcriptomic changes were analyzed by microarray profiling and real-time reverse transcription polymerase chain reaction. The mucosal microbiome was studied by high throughput pyrosequencing of 16S rRNA. Maternal MD supplementation induced a striking susceptibility to colitis in offspring. This phenotype was associated with colonic mucosal DNA methylation and expression changes. Metagenomic analyses did not reveal consistent bacteriomic differences between P30 and P90, but showed a prolonged effect of the diet on the offspring mucosal microbiome. In conclusion, maternal MD supplementation increases offspring colitis susceptibility that associates with persistent epigenetic and prolonged microbiomic changes. These findings underscore that epigenomic reprogramming relevant to mammalian colitis can occur during early development in response to maternal dietary modifications.

Topics: Animals; Bacteria; Betaine; Choline; Colitis; Dietary Supplements; Disease Susceptibility; DNA Methylation; Epigenesis, Genetic; Female; Folic Acid; Humans; Intestinal Mucosa; Male; Maternal Nutritional Physiological Phenomena; Metagenome; Mice; Mice, Inbred C57BL; Pedigree; Pregnancy; Prenatal Exposure Delayed Effects; Vitamin B 12

Topics: Adult; Anemia, Hypochromic; Arthroplasty, Replacement, Hip; Chronic Disease; Colitis; Drug Therapy, Combination; Epoetin Alfa; Erythropoietin; Ferric Compounds; Folic Acid; Humans; Intestinal Polyps; Leucovorin; Malabsorption Syndromes; Male; Methotrexate; Osteoarthritis, Hip; Preoperative Care; Recombinant Proteins; Rectal Diseases; Remission Induction; Spondylitis, Ankylosing; Time Factors; Vitamin B 12; Vitamin B 12 Deficiency

Bile acid studies were performed in patients with Crohn's disease, radiologically confined to the colon. The bile acid pool size of 10 patients with isolated Crohn's colitis was significantly lower than that of 10 normal control subjects (P less than 0.001) and of 10 ulcerative colitis patients (P less than 0.005). Measurements of 14C-excretion in breath and in 24 hours stool collections after the administration of 5 muCi 14C-glycocholate showed a normal 14C-excretion in breath and usually a markedly increased loss of 14C in the stool (greater than 7% of the dose). The simultaneous administration of 5 muCi 3H-polyethylene glycol MW 4000 (3H-PEG) as a marker indicated that the 14C/3H ratio in the patients with Crohn's colitis was significantly greater than in a control series of patients with diarrhoea not due to bile acid malabsorption. Studies on the composition of duodenal bile showed a significantly decreased concentration of deoxycholic acid in duodenal bile. These observations suggest bile acid malabsorption in patients with Crohn's disease apparently confined to the colon.

Topics: Adolescent; Adult; Bile Acids and Salts; Breath Tests; Colitis; Colitis, Ulcerative; Crohn Disease; Deoxycholic Acid; Feces; Female; Glycocholic Acid; Humans; Intestinal Absorption; Male; Middle Aged; Vitamin B 12

A series of 130 consecutive outpatients with recurrent aphthous stomatitis were screened at the oral medicine department, Glasgow Dental Hospital, for deficienciesin vitamin b12, folic acid, and iron. In 23 patients (17.7%) such deficiencies werefound; five were deficient in vitamin B12, seven in folic acid, and 15 in iron. Four had more than one deficiency. Out of 130 controls matched for age and sex 11 (8.5%) were found to have deficiencies. The 23 deficient patients with recurrent aphthaewere treated with specific replacement therapy, and all 130 patients were followed up for at least one year. Of the 23 patients on replacement therapy 15 showed complete remission of ulceration and eight definite improvement. Of the 107 patientswith no deficiency receiving local symptomatic treatment only 33 had a remission or wereimproved. This difference was significant (P less than 0.001). Most patients withproved vitamin B12 or folic acid deficiency improved rapidly on replacement therapy;those with iron deficiency showed a less dramatic response. The 23 deficient patientswere further investigated to determine the cause of their deficiencies and detect the presence of any associated conditions. Four were found to have Addisonian perniciousanaemia. Seven had a malabsorption syndrome, which in five proved to be a gluten-induced enteropathy. In addition, there were single patients with idiopathic proctocolitis, diverticular disease of the colon, regional enterocolitis, and adenocarcinoma of thecaecum. We suggest that the high incidence of deficiencies found in this series andthe good response to replacement therapy shows the need for haematological screening of such patients.

Topics: Adenocarcinoma; Adolescent; Adult; Aged; Anemia, Pernicious; Cecal Neoplasms; Child; Colitis; Crohn Disease; Diverticulum, Colon; Female; Folic Acid; Folic Acid Deficiency; Humans; Iron; Iron Deficiencies; Malabsorption Syndromes; Male; Middle Aged; Proctitis; Recurrence; Stomatitis, Aphthous; Vitamin B 12; Vitamin B 12 Deficiency

Topics: Adolescent; Adult; Aged; Celiac Disease; Colitis; Crohn Disease; Dietary Fats; Feces; Female; Humans; Ileum; Intestinal Absorption; Lipids; Male; Middle Aged; Postoperative Complications; Recurrence; Schilling Test; Serum Albumin; Vitamin B 12; Xylose

Topics: Adult; Aged; Colitis; Enteritis; Female; Humans; Intestinal Absorption; Male; Middle Aged; Placenta Diseases; Pregnancy; Vitamin B 12

Topics: Adolescent; Adult; Ascorbic Acid; Child; Colitis; Female; Humans; Male; Middle Aged; Neurologic Manifestations; Niacinamide; Pantothenic Acid; Pyridoxine; Skin Manifestations; Tetracycline; Vitamin B 12

Topics: Adult; Colitis; Female; Gastric Mucosa; Gastritis; Histidine; Humans; Intestinal Mucosa; Male; Methods; Pantothenic Acid; Peptic Ulcer; Vitamin B 12

Topics: Atrophy; Biopsy; Carbohydrate Metabolism; Colitis; Colitis, Ulcerative; Electrons; Feces; Fluids and Secretions; Gastritis; Intestinal Absorption; Intestine, Small; Lipid Metabolism; Microscopy; Microscopy, Electron; Pathology; Stomach; Vitamin B 12; Xylose

Topics: Anemia; Colitis; Corrinoids; Hematinics; Humans; Iron; Serum; Stomach; Stomach Diseases; Vitamin B 12; Vitamins

Topics: Colitis; Colitis, Ulcerative; Corrinoids; Hematinics; Humans; Vitamin B 12; Vitamin B Complex

Topics: Chronic Disease; Colitis; Hematinics; Humans; Vitamin B 12

Topics: Colitis; Colitis, Ulcerative; Hematinics; Humans; Vitamin B 12