vasoactive-intestinal-peptide and Splenic-Diseases

To observe the effect of Sijunzi Decoction on secretion disorder of salivary amylase in splenasthenic rat and its mechanism.. The model group rats received reserpine 0.5 mg/kg through subcutaneous injection while the control group rats received the same volume of saline for 8 days. After being modeled, the model group were divided into treatment group and model control group, treatment group were given orally Sijunzi Decoction, model control group and normal group were fed the same amount of distilled water for 4 weeks. The animal were anaesthetized and the left parotid was removed, the wounds were sutured. When the animals were awake but drowsy, 20 microL 10% glacial acetic acid was applied on the apex of the tongue once a minute for 30 minutes, removed the right parotid gland of the animals. The samples were frozen and amylase activity and VIP, cyclic adenosine monophosphate (cAMP) content and VAMP-8, SNAP-23 protein expression in the parotid glands were detected.. Change of sAA in parotid acinar was not significantly different between treatment group and normal groups, but higher in model control groups after acid stimulation. The VIP and PKA contents were not significantly different among three groups. VIP, cAMP content and PKA activity increased significantly in normal group while VIP increased slightly, cAMP and PKA activity decreased in model control groups, which returned to some degrees in treatment group after acid stimulation. Expression of VAMP-8 protein was not significantly different between treatment group and model control groups, while expression of SNAP-23 was lower in model control groups, expression of VAMP-8 and SNAP-23 was higher in treatment group than which in model control groups.. Sijunzi Decoction has a certain effect on secretion disorder of salivary amylase in splenasthenic rat, which mechanism may be related to recover changes of VIP-cAMP signal pathway in the splenasthenic rat's parotid gland cells,including increase VIP content and expression of VAMP-8 and SNAP-23.

Topics: Amylases; Animals; Cyclic AMP; Disease Models, Animal; Drug Combinations; Drugs, Chinese Herbal; Male; Parotid Gland; Plants, Medicinal; Random Allocation; Rats; Rats, Sprague-Dawley; Reserpine; Signal Transduction; Splenic Diseases; Vasoactive Intestinal Peptide

To explore the mechanism of spleen deficiency (SD) by studying the relationship of gastro-intestinal peptides level and ileal electro-mechanical activity of SD rats and cold restrain rats.. (1) spleen deficiency (SD) model was established by feeding Houpou:Zhishi: Dahuang in the ratio of 3:3:2, 3 ml/time, for 42 days. (2) The cold restrain stress model: Animals were restrained on grille and placed in a cool water at 18 degrees for 3 h. (3) Substance P (SP) and vasoactive intestinal peptide (VIP) levels in all layers of initial part of ileum and blood in rats were measured by radioimmunoassays (RIA) while changes of electric activity and motility in ileum of rats were recorded with electrode and strain gauge.. SP levels in ileum and blood of experimental SD rats were significantly higher than that of the control groups (9.89+/-5.65 vs 1.22+/-1.18, P<0.005, in ileum; 22.7+/-3.95 vs 6.60+/-1.47, P<0.001, in blood) while the VIP levels of the SD rats were significantly lower than that of the controls (3.50+/-2.01 vs 9.10+/-4.91, P<0.05, in ileum; 229.8+/-62.4 vs 560.4+/-151.3, P<0.001, in blood). As compared with the controls, the average frequency of slow electric waves (21.3+/-0.96 vs 18.2+/-2.28, P<0.05) and motility (21.5+/-0.58 vs 18+/-2.65, P<0.005) of SD rats increased obviously and the frequency of fast waves of SD rat also increased. In spontaneous recovery cases, SP levels recovered significantly (compared with the SD groups, 2.99+/-0.62 vs 9.89+/-5.65, P<0.001, in ileum; 14.4+/-4.22 vs 22.7+/-3.95, P<0.001, in blood) but did not drop to normal. After the SD rats treated with Chinese herbs (Jiawei Sijun zi Tang), SP improved (compared with SD cases, 2.20+/-1.25 vs 9.89+/-5.65, (P<0.001), in ileum; 10.7+/-1.88 vs 22.7+/-3.95, (P<0.001), in blood) and VIP in blood also improved (compared with SD rats, 485.7+/-229.0 vs 229.8+/-62.4, P<0.01) while the amplitude of motility decreased apparently (compared with the SD rats, 0.64+/-0.096 vs 0.89+/-0.15, P<0.01). The ileal SP levels of cool stress didn't change while the ileal VIP levels of cool stress became significantly lower than that of the control groups (2.87+/-0.87 vs 9.10+/-4.91, P<0.01). The blood SP levels of cool stress were significantly higher (15.60+/-1.83 vs 6.60+/-1.47, P<0.001) whereas the blood VIP levels of cool stress were significantly lower than that of the control group (153.4+/-70.46 vs 560.4+/-151.30, P<0.001).. Changes of SP and VIP levels in initial part of ileum and blood of SD rats and cool stress rats may be closely related to the gastrointestinal motility disorders presented in SD and cool stress rats. the Chinese herbs (Jiawei Sijun zi Tang) currently used have partially therapeutic effect.

Topics: Animals; Electrophysiology; Ileum; Male; Rats; Rats, Wistar; Splenic Diseases; Substance P; Vasoactive Intestinal Peptide

By means of radioimmunoassay, the mucosal substance P (SP) and vasoactive intestinal polypeptide (VIP) concentration in distal ileum, transverse colon and sigmoid colon in 30 patients of chronic diarrhea with splenic weakness was determined and compared with that in 28 patients of chronic diarrhea without splenic weakness and in 15 controls without chronic diarrhea. In patients of chronic diarrhea with splenic weakness, the SP contents in the mucosa of distal ileum (120.95 +/- 90.70 pg/mg wet weight) was significantly increased compared with that in controls without diarrhea (47.86 +/- 35.49 pg/mg wet weight) and in chronic diarrhea without splenic weakness (52.50 +/- 42.49 pg/mg wet weight), P less than 0.01 and 0.01 less than P less than 0.05 respectively. The VIP contents of sigmoid mucosa in patients of chronic diarrhea with splenic weakness (510.63 +/- 265.22 pg/mg wet weight) was markedly increased in comparison of that in controls without chronic diarrhea (308.67 +/- 204.49 pg/mg wet weight), 0.01 less than P less than 0.05, and was not significantly augmented compared with that in chronic diarrhea without splenic weakness (398.97 +/- 240.80 pg/mg wet weight), but the tendency of increase was present. Our results suggested that the increased SP and VIP in patients of chronic diarrhea with splenic weakness might be closely related to the symptom of chronic diarrhea. According to the general function of VIP, the authors predicted that VIP might play a more important role in the pathogenetic action of chronic diarrhea with splenic weakness.

Topics: Adult; Chronic Disease; Diarrhea; Female; Humans; Intestinal Mucosa; Male; Splenic Diseases; Substance P; Vasoactive Intestinal Peptide