vasoactive-intestinal-peptide and Leukemia--Lymphoid

vasoactive-intestinal-peptide has been researched along with Leukemia--Lymphoid* in 2 studies

Other Studies

2 other study(ies) available for vasoactive-intestinal-peptide and Leukemia--Lymphoid

ArticleYear
Autocrine proliferative effect of ghrelin on leukemic HL-60 and THP-1 cells.
    The Journal of endocrinology, 2007, Volume: 192, Issue:1

    Ghrelin is a 28 amino acid peptide hormone that is mainly produced by the stomach, but also by several tissues and tumors. Ghrelin is octanoylated on the Ser(3), but is also detected as a des-acylated form. Only the acylated ghrelin activates the GH secretagogue receptor (GHS-R) type 1a to stimulate GH release, and regulate food intake and energy metabolism. For the first time, we report that ghrelin and des-acyl ghrelin are present in human promyelocytic HL-60, monocytic THP-1 and lymphoblastic SupT1 cell lines. The human leukemic cell lines did not express the functional GHS-R 1a, whereas they expressed GHS-R 1b, a truncated variant of the receptor. Leukemic cell proliferation was not modified by the addition of octanoylated or des-acyl ghrelins. However, THP-1 and HL-60 cell proliferations were inhibited by SB801, an antibody directed against the N-terminal octanoylated portion of ghrelin, suggesting that octanoylated ghrelin stimulates cell proliferation via an autocrine pathway involving an as yet unidentified ghrelin receptor. Both octanoylated and des-acyl ghrelins did not alter the basal adenylate cyclase activity. Treatments of THP-1 and SupT1 cells by both octanoylated and des-acyl ghrelins did not modify the adenylate cyclase activity in response to vasoactive intestinal peptide, suggesting that ghrelin is unlikely to modulate the anti-inflammatory and differentiating properties of vasoactive intestinal peptide.

    Topics: Adenylyl Cyclases; Autocrine Communication; Carboxylesterase; Cell Line, Tumor; Cell Proliferation; Ghrelin; HL-60 Cells; Humans; Leukemia; Leukemia, Lymphoid; Leukemia, Monocytic, Acute; Leukemia, Promyelocytic, Acute; Peptide Hormones; Receptors, G-Protein-Coupled; Receptors, Ghrelin; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger; Vasoactive Intestinal Peptide

2007
Demonstration of a functional receptor for vasoactive intestinal polypeptide on Molt 4b T lymphoblasts.
    Regulatory peptides, 1983, Volume: 6, Issue:1

    Viable human T lymphoblasts derived from the "Molt 4b" cell line have been shown to possess functional plasma membrane receptors for vasoactive intestinal polypeptide (VIP). Specific binding of 125I-VIP to these lymphoblasts is rapid, reversible and linearly dependent on the number of cells present. Analysis of binding at 17 degrees C reveals a single class of high affinity binding sites over the concentration range of 10(-7) to 10(-11) M VIP (KD = 7.3 +/- 1.3 nM). The Bmax of 0.24 +/- 0.07 nM extrapolates to 15 000 +/- 4000 sites/cell. The binding of 125I-VIP to T lymphoblasts is highly specific; secretin and glucagon, peptides of similar molecular weight which show sequence homology with VIP, are unable to competitively inhibit binding of 125I-VIP to Molt 4b lymphoblasts. VIP activates adenylate cyclase in membrane preparations from Molt 4b lymphoblasts and increases cAMP in intact cells. Half maximal activation in both membrane preparations and intact cells occurs at 5 nM VIP. This demonstration of a functional receptor for VIP suggests that the Molt 4b lymphoblastic cell line may be a useful model system in which to study neuropeptide modulation of T lymphocyte function.

    Topics: Adenylyl Cyclases; Binding, Competitive; Cell Line; Cell Membrane; Cyclic AMP; Gastrointestinal Hormones; Humans; Kinetics; Leukemia, Lymphoid; Receptors, Cell Surface; Receptors, Vasoactive Intestinal Peptide; T-Lymphocytes; Vasoactive Intestinal Peptide

1983