vasoactive-intestinal-peptide and Dermatitis

The aim of this study was to evaluate the antiinflammatory effect of oatmeal extract oligomer on skin fragments stimulated by a neuromediator, vasoactive intestinal peptide (VIP). Skin fragments (from plastic surgery) were maintained in survival conditions for 6 h. To induce inflammation, VIP was placed in contact with dermis by culture medium. Histological analysis was then performed on hematoxylin- and eosin-stained slides. Edema was evaluated with semiquantitative scores. Vasodilation was studied by quantifying the percentage of dilated vessels according to scores and by measuring their surface by morphometrical image analysis. TNF-alpha dosage was made on culture supernatants. Vasodilation was significantly increased after application of VIP. After treatment with oatmeal extract oligomer, the mean surface of dilated vessels and edema were significantly decreased compared with VIP-treated skin. Moreover, treatment with this extract decreased TNF-alpha.

Topics: Adult; Avena; Dermatitis; Edema; Female; Humans; Middle Aged; Organ Culture Techniques; Phytotherapy; Plant Extracts; Skin; Tumor Necrosis Factor-alpha; Vasoactive Intestinal Peptide; Vasodilation

Neuropeptides (NP) are protein compounds contained both in the central and peripheral nervous system. They can be antidromically released from sensory nerves and are implicated in the so-called neurogenic inflammation. They also exert a number of functions within the immune system and are thought to act as trophic as well as mitogenic substances. Several NP have been detected in human skin by immunohistochemical and radioimmunological techniques, and recent reports have demonstrated that NP could be involved in the mechanisms of certain dermatoses. The involvement of NP in either physiological or pathophysiological skin conditions is discussed. Moreover, a few questions, which still need to be addressed, are raised, and future directions this field of research should take are outlined.

Topics: Calcitonin Gene-Related Peptide; Dermatitis; Humans; Inflammation; Neuropeptides; Skin; Skin Physiological Phenomena; Somatostatin; Stress, Psychological; Substance P; Vasoactive Intestinal Peptide

Immunoreactivity (IR) of substance P (SP) and Vasoactive Intestinal Peptide (VIP) were determined by radioimmunoassay in serum of 56 patients with inflammatory skin diseases, in blister fluid of 40 patients with spontaneous blisters and 31 subjects with induced skin blisters. Serum concentrations of SP-IR and VIP-IR were mostly low or non-detectable. Spontaneous blisters contained high amounts of SP-IR, particularly in bullous pemphigoid and in some inflammatory dermatoses, while VIP-IR levels usually were low. Suction blisters from inflamed but not from normal skin often contained SP-IR but more seldom VIP-IR.

Topics: Adolescent; Adult; Aged; Blister; Chromatography, High Pressure Liquid; Dermatitis; Female; Humans; Male; Middle Aged; Radioimmunoassay; Skin Diseases, Vesiculobullous; Substance P; Vasoactive Intestinal Peptide

Topics: Animals; Arteries; Cats; Dermatitis; Dogs; Gastrointestinal Hormones; Guinea Pigs; Haplorhini; Humans; Nerve Fibers; Neurotensin; Nociceptors; Pruritus; Rats; Skin; Substance P; Vasoactive Intestinal Peptide; Veins