valinomycin has been researched along with Amyotrophic-Lateral-Sclerosis* in 1 studies
1 other study(ies) available for valinomycin and Amyotrophic-Lateral-Sclerosis
| Article | Year |
|---|---|
Expression of a Cu,Zn superoxide dismutase typical of familial amyotrophic lateral sclerosis induces mitochondrial alteration and increase of cytosolic Ca2+ concentration in transfected neuroblastoma SH-SY5Y cells.
We have set up a model system for familial amyotrophic lateral sclerosis (FALS) by transfecting human neuroblastoma cell line SH-SY5Y with plasmids directing constitutive expression of either wild-type human Cu,Zn superoxide dismutase (Cu,ZnSOD) or a mutant of this enzyme (G93A) associated with FALS. We have tested mitochondrial function and determined cytosolic Ca2+ concentration in control cells (untransfected) and in cells expressing either wild-type Cu,ZnSOD or G93A. We report that G93A induces a significant loss of mitochondrial membrane potential, an increased sensitivity toward valinomycin and a parallel increase in cytosolic Ca2+ concentration. The above phenomena are not related to total Cu,ZnSOD content and activity in the cell. Topics: Amyotrophic Lateral Sclerosis; Calcium; Cloning, Molecular; Cytosol; Humans; Intracellular Membranes; Membrane Potentials; Mitochondria; Neuroblastoma; Polymerase Chain Reaction; Recombinant Proteins; Superoxide Dismutase; Transfection; Tumor Cells, Cultured; Valinomycin | 1997 |