uroguanylin and Colitis--Ulcerative

uroguanylin has been researched along with Colitis--Ulcerative* in 2 studies

Other Studies

2 other study(ies) available for uroguanylin and Colitis--Ulcerative

ArticleYear
Colonic epithelial cell diversity in health and inflammatory bowel disease.
    Nature, 2019, Volume: 567, Issue:7746

    The colonic epithelium facilitates host-microorganism interactions to control mucosal immunity, coordinate nutrient recycling and form a mucus barrier. Breakdown of the epithelial barrier underpins inflammatory bowel disease (IBD). However, the specific contributions of each epithelial-cell subtype to this process are unknown. Here we profile single colonic epithelial cells from patients with IBD and unaffected controls. We identify previously unknown cellular subtypes, including gradients of progenitor cells, colonocytes and goblet cells within intestinal crypts. At the top of the crypts, we find a previously unknown absorptive cell, expressing the proton channel OTOP2 and the satiety peptide uroguanylin, that senses pH and is dysregulated in inflammation and cancer. In IBD, we observe a positional remodelling of goblet cells that coincides with downregulation of WFDC2-an antiprotease moleculeĀ that we find to be expressed by goblet cellsĀ and that inhibits bacterial growth. In vivo, WFDC2 preserves the integrity of tight junctions between epithelial cells and prevents invasion by commensal bacteria and mucosal inflammation. We delineate markers and transcriptional states, identify a colonic epithelial cell and uncover fundamental determinants of barrier breakdown in IBD.

    Topics: Animals; Biomarkers; Colitis, Ulcerative; Colon; Epithelial Cells; Genetic Predisposition to Disease; Goblet Cells; Health; Humans; Hydrogen-Ion Concentration; Inflammatory Bowel Diseases; Intestinal Mucosa; Ion Channels; Male; Mice; Natriuretic Peptides; Proteins; Single-Cell Analysis; Stem Cells; Tight Junctions; Transcription, Genetic; WAP Four-Disulfide Core Domain Protein 2

2019
Expression of guanylate cyclase-C, guanylin, and uroguanylin is downregulated proportionally to the ulcerative colitis disease activity index.
    Scientific reports, 2016, 04-29, Volume: 6

    The transmembrane receptor guanylate cyclase-C (GC-C) signaling pathway has been implicated in several gastrointestinal disorders. Activation of GC-C via guanylin (Gn) and uroguanylin (Ugn) regulates intestinal fluid and electrolyte homeostasis. However, how it regulates the pathogenesis of inflammatory bowel disease (IBD) is still unclear. Here, we investigated the activation of GC-C signaling in ulcerative colitis (UC) of different clinical severities. A total of 60 UC patients and 20 normal controls were recruited. Evaluation of the UC disease activity index (DAI) was performed using a modified Mayo scoring system. The expression of GC-C, Gn and Ugn in the colonic mucosa was measured by quantitative real-time PCR and Western blot. We found that the UC patients had significantly lower expression of GC-C, Gn and Ugn than the controls. Furthermore, there were significant differences for GC-C, Gn and Ugn expression for the UC groups of Grade 1, 2 and 3, and their expression levels were reduced with increases in their DAI. Taken together, our results demonstrate that GC-C, Gn and Ugn are downregulated in UC, and this downregulation is more significant with aggravation of the clinical condition. Therefore, the GC-C signaling pathway may be implicated in the progression of UC.

    Topics: Adult; Colitis, Ulcerative; Down-Regulation; Female; Gastrointestinal Hormones; Humans; Male; Middle Aged; Natriuretic Peptides; Receptors, Atrial Natriuretic Factor; Signal Transduction

2016