urobilin and Anemia--Hemolytic

Topics: Anemia; Anemia, Hemolytic; Bile Pigments; Bilirubin; Biochemical Phenomena; Biochemistry; Clinical Laboratory Techniques; Hemoglobins; Humans; Infant, Newborn; Infant, Newborn, Diseases; Jaundice; Jaundice, Neonatal; Metabolism; Physiology; Urobilin

Topics: Anemia, Hemolytic; Cholestasis; Humans; Liver Diseases; Reference Values; Spectrophotometry; Urobilin; Urobilinogen

The detailed analysis of faecal bile pigments by high-performance liquid chromatography is described. Non-aqueous reversed-phase systems with acetonitrile-dimethyl sulphoxide or acetonitrile-dimethyl sulphoxide-methanol as the mobile phase on C1, C8 or C18-bonded silica are used for the group separation of verdinoids, violinoids and urobilinoids. A silica column, with acetonitrile-water-tetraethylene-pentamine as mobile phase, separates the laevorotatory stercobilin (C33H46N4O6) and half-stercobilin (C33H44N4O6) from the optically inactive urobilin (C33H42N4O6). The diastereoisomers are resolved by converting the urobilinoids into their dimethyl esters before chromatography on a silica column with n-heptane-methyl acetate-methanol containing 1% of diethylamine as the solvent system.

Topics: Anemia, Hemolytic; Bile Pigments; Chromatography, High Pressure Liquid; Feces; Humans; Urobilin

Topics: Adult; Anemia, Hemolytic; Bilirubin; Blood Cell Count; Blood Chemical Analysis; Blood Proteins; Blood Transfusion; Bone Marrow Examination; Cephalothin; Cholangitis; Cholestasis; Diagnosis, Differential; Escherichia coli; Escherichia coli Infections; Female; Humans; Jaundice; Kanamycin; Leukocytes; Proteinuria; Sepsis; Urine; Urobilin

Topics: Anemia, Hemolytic; Erythrocytes; Hemolysis; Humans; Urobilin; Wound Infection

Topics: Anemia, Hemolytic; Chemical Phenomena; Chemistry; Chromates; Chromatography, Thin Layer; Feces; Humans; Indicators and Reagents; Iron; Molecular Weight; Spectrum Analysis; Urobilin