umi-77 has been researched along with Prostatic-Neoplasms* in 1 studies
1 other study(ies) available for umi-77 and Prostatic-Neoplasms
| Article | Year |
|---|---|
Synthesis and evaluation of a UMI-77-based fluorescent probe for selective detecting Mcl-1 protein and imaging in living cancer cells.
Development of efficient fluorescent probes for detecting the overexpressed Mcl-1 protein in living cells is imperative for the diagnosis and treatment of cancers. In this paper, a new UMI-77 based fluorescent probe (DNSH), was synthesized and characterized. DNSH bound to the hydrophobic pockets of Mcl-1 protein tightly and the binding affinity was 20-fold higher than that of previous developed Mcl-1 probe. DNSH exhibited specific fluorescence response to Mcl-1 protein rather than other proteins. In the presence of Mcl-1 protein, fluorescence emission of DNSH can be switched on. Furthermore, fluorescence colocalization experiment demonstrated that DNSH can be successfully used for imaging mitochondrial Mcl-1 protein in human prostate cancer cells without a washing process. These results showed that DNSH may find useful applications in biological research such as tracking Mcl-1 protein in living biological specimens. Topics: Dose-Response Relationship, Drug; Fluorescent Dyes; Humans; Male; Models, Molecular; Molecular Structure; Myeloid Cell Leukemia Sequence 1 Protein; Optical Imaging; Prostatic Neoplasms; Structure-Activity Relationship; Sulfonamides; Thioglycolates | 2021 |