uleine and Inflammation

The aim of this work was to investigate the anti-inflammatory activities of the uleine-rich fraction extracted from the barks of Himatanthus lancifolius (Muell. Arg.) Woodson (Apocynaceae). To achieve this, we focused on its in vitro effects on some steps of the inflammatory response using peripheral human leukocytes. The results presented herein show that the uleine-rich fraction significantly inhibits the migration of casein-induced granulocytes and their adhesion to fibronectin and vitronectin, along with mononuclear cells, by down-regulating the expression of alpha 4beta1 and alpha5beta1 integrins. The data suggest that H. LANCIFOLIUS has the potential of interferring with leukocyte trafficking through its uleine-rich fraction, emphasizing its usefulness in inflammatory conditions. DEXA:dexamethasone disodium phosphate FN:fibronectin PMN:polymorphonuclear URF:uleine-rich fraction VN:vitronectin.

Topics: Alkaloids; Apocynaceae; Bridged-Ring Compounds; Caseins; Cell Adhesion; Cell Migration Assays, Leukocyte; Chemotaxis; Fibronectins; Humans; Inflammation; Integrin alpha4beta1; Integrin alpha5beta1; Leukocytes; Phytotherapy; Plant Bark; Plant Extracts; Vitronectin