ubiquinone and Swine-Diseases

The aim of this study was to evaluate the effect of low-energy laser irradiation, coenzyme Q10 and vitamin E supplementation on the apoptosis of macrophages and muscle precursor cells during skeletal muscle regeneration after bupivacaine-induced injury. The experiment was conducted on 75 gilts, divided into 5 experimental groups: I--control, II--low-energy laser irradiation, III--coenzyme Q10, IV--coenzyme Q10 and vitamin E, V--vitamin E. Muscle necrosis was induced by injection of 0.5% bupivacaine hydrochloride. The animals were euthanized on subsequent days after injury. Samples were formalin fixed and processed routinely for histopathology. Apoptosis was detected using the TUNEL method. The obtained results indicate that low-energy laser irradiation has a beneficial effect on macrophages and muscle precursor cell activity during muscle post-injury regeneration and protects these cells against apoptosis. Vitamin E has a slightly lower protective effect, limited mainly to the macrophages. Coenzyme Q10 co-supplemented with vitamin E increases the activity of macrophages and muscle precursor cells, myotube and young muscle formation. Importantly, muscle precursor cells seem to be more sensitive to apoptosis than macrophages in the environment of regenerating damaged muscle.

Topics: Animals; Antioxidants; Apoptosis; Bupivacaine; Dose-Response Relationship, Radiation; Drug Therapy, Combination; Female; Lasers; Muscle, Skeletal; Muscular Diseases; Swine; Swine Diseases; Ubiquinone; Vitamin E

A new bacterial strain, designated as FF42(T), was isolated from feces of domestic pigs-collected from Suwon, Korea-and was characterized to determine its taxonomic position. Strain FF42(T) was observed to be Gram negative, aerobic, non-spore forming, motile, and rod-shaped cells. Based on the phylogenetic and 16S rRNA sequence analyses, it was revealed that strain FF42(T) belonged to the genus Comamonas. The highest degree of sequence similarities was determined to be with Comamonas zonglianii BF-3(T) (96.3 %), Comamonas composti CC-YY287(T) (96.1 %), and Comamonas nitrativorans 23310(T) (95.9 %), while showing less than 95.6 % identity with the remaining Comamonas species. Growth of strain FF42(T) occurred between 25 and 40 °C (optimum, 28 °C) and at pH of 5-9 (optimum, pH 6.0). It grew in the presence of 0-3 % (w/v) NaCl while minimally tolerating at 3 % (w/v) NaCl. Biochemical and physiological tests revealed phenotypic differentiation of strain FF42(T) to other members of the genus Comamonas. The predominant quinone is ubiquinone (Q-8). The major cellular fatty acids were C10:0 3OH, C16:0, summed feature 3 (C16:1 ω7c/C16:1 ω6c), and summed feature 8 (C18:1 ω6c/C18:1 ω7c), all of which have previously been reported to occur in the species of the genus Comamonas. The G+C molar content for strain FF42(T) is 60.2 mol %. Based on phylogenetic and phenotypic analyses, strain FF42(T) (=KEMC 1002-058(T)=JCM 17561(T)) is clearly referred to be a novel species for the genus Comamonas, for which the name Comamonas faecalis sp. nov. is proposed.

Topics: Animals; Base Composition; Base Sequence; Comamonas; DNA, Bacterial; DNA, Ribosomal; Fatty Acids; Feces; Gram-Negative Bacterial Infections; Molecular Sequence Data; Phenotype; Phylogeny; RNA, Ribosomal, 16S; Sequence Analysis, DNA; Sus scrofa; Swine; Swine Diseases; Ubiquinone

The aim of the study was to observe the effect of coenzyme Q10 and vitamin E supplementation on the course of the regeneration process of the longissimus lumborum muscle after bupivacaine-induced myonecrosis as well as to determine the correlation between the level of those substances in plasma and their levels in damaged and non-damaged muscular tissue in pigs. The obtained results indicate that the course of regeneration of a damaged muscle is affected to a higher extent by coenzyme Q10 than by vitamin E. The administration of coenzyme Q10 and vitamin E has a significant impact on the increase in the level of those substances in damaged muscles and plasma of animals.

Topics: Anesthetics, Local; Animal Feed; Animals; Antioxidants; Bupivacaine; Coenzymes; Female; Muscle, Skeletal; Muscular Diseases; Necrosis; Regeneration; Swine; Swine Diseases; Ubiquinone; Vitamin E