trypsinogen and Tongue-Neoplasms

Enhanced proteolysis and altered tight junction (TJ) proteins associate with carcinoma invasion. We hypothesized that trypsin-2, a tumor-associated serine proteinase, induces tongue carcinoma invasion by activating pro-membrane type-1 matrix metalloproteinase (MT1-MMP) and disturbing the TJs. The effects of invasion were analyzed using trypsin-2 over-expressing human tongue squamous cell carcinoma cells (Try2-HSC-3) in vitro and in vivo. The invasion of Try2-HSC-3 cells was increased in mouse xenografts and human organotypic model. Trypsin-2 activated proMT1-MMP, as well as altered the expression of TJ protein claudin-7. In conclusion, trypsin-2 over-expression enhanced tongue carcinoma cell invasion by various genetic and proteolytic mechanisms.

Topics: Animals; Carcinoma, Squamous Cell; Cell Line, Tumor; Cell Movement; Claudins; Enzyme Activation; Female; Gene Expression; Gene Expression Profiling; Humans; Matrix Metalloproteinase 14; Mice; Mice, Nude; Neoplasm Invasiveness; Protein Precursors; Tight Junctions; Tongue Neoplasms; Trypsin; Trypsinogen; Tumor Burden; Xenograft Model Antitumor Assays