trypsinogen and Intestinal-Obstruction

Detection of elevated levels of immunoreactive trypsinogen (IRT) in dried neonatal blood spots has been used as a screening test for cystic fibrosis. In other cystic fibrosis newborn-screening studies, a sweat chloride test is generally performed only if an infant has a persistent IRT level above a selected cutoff value on both the initial and subsequent specimens. Neither the timing of the second specimen nor the value of the cutoff point for the second specimen has been comprehensively evaluated. In this randomized, controlled study, 145,024 infants were screened in the neonatal period for cystic fibrosis using the 99.8 percentile (180 ng/mL) as the neonatal cutoff point. A total of 129 infants had elevated neonatal IRT levels and had negative results on sweat tests (false-positive by IRT screening). A total of 54 children with cystic fibrosis were identified in the screened and comparison groups. Excluding patients with meconium ileus, 4 infants with cystic fibrosis had neonatal IRT values less than 180 ng/mL, and an additional 9 infants with cystic fibrosis had values decline to less than 180 ng/mL within the first 2 1/2 months of age. The IRT values of infants with and without cystic fibrosis overlapped considerably beyond 30 days of age. These findings suggest that further refinement of cystic fibrosis screening methodology will be necessary to achieve an acceptable sensitivity and specificity.

Topics: Aging; Antibodies; Cystic Fibrosis; Humans; Incidence; Infant; Infant, Newborn; Infant, Premature; Intestinal Obstruction; Mass Screening; Meconium Aspiration Syndrome; Radioimmunoassay; Randomized Controlled Trials as Topic; Trypsinogen; Wisconsin

Phasing of heterozygous alleles is critical for interpretation of

Topics: Cystic Fibrosis; Cystic Fibrosis Transmembrane Conductance Regulator; Humans; Infant, Newborn; Intestinal Obstruction; Meconium; Meconium Ileus; Trypsin; Trypsinogen

Topics: Humans; Infant, Newborn; Intestinal Obstruction; Meconium; Trypsinogen

Topics: Electrophoresis; Endopeptidases; Enzyme Activation; Humans; Intestinal Obstruction; Isoelectric Focusing; Pancreatic Juice; Trypsin; Trypsinogen