triolein and Eye-Diseases

Triolein emulsion is under investigation for supplemental use to open the blood-brain barrier during chemotherapy. The effects of triolein emulsion on the blood-retinal barrier (BRB) were investigated.. Fat emboli were induced in 20 cats by injecting triolein emulsion through the carotid artery. At 30 min, 4, 12 and 48 h after embolization, electroretinography (ERG) and fluorescein angiography (FA) were performed. The eyeballs were enucleated for transmission electron-microscopic study.. FA revealed multiple leaking points at 30 min, and prominent diffuse leakage at 4 h when scotopic b-waves showed significant differences between the study and control eyes. Multiple focal disruptions of the blood vessels by fat vacuoles were found with electron microscopic study. ERG improved at 12 and 48 h, and the BRB appeared to be recovered on FA and electron microscopic studies after 48 h.. An experimental embolism with triolein emulsion disrupted the blood retinal barrier. Delayed maximal change was observed, and it could be implicated in the latent interval of clinical fat embolism syndrome.

Topics: Animals; Blood-Retinal Barrier; Capillary Permeability; Cats; Dark Adaptation; Disease Models, Animal; Electron Microscope Tomography; Electroretinography; Embolism, Fat; Emulsions; Eye Diseases; Fluorescein Angiography; Time Factors; Triolein

In human fat embolism we often find different grades of changes in both eyes of a patient. This was experimentally verified in rabbits. The anesthetized animals were held in lateral position so that one eye was continuously higher than the other. Labelled fat was injected intravenously, and then the activity in each eye was measured by a scintillation-counter. By this method a significantly higher rate of fat embolism was found in the upper eye.

Topics: Animals; Embolism, Fat; Eye Diseases; Female; Gravitation; Iodine Radioisotopes; Male; Rabbits; Scintillation Counting; Triolein