triolein and Digestive-System-Diseases

To differentiate maldigestion from malabsorption of fat, we compared the radioactivity of triglyceride with non-esterified fatty acid in fecal fat after ingestion of radiochemically purified 131I-triolein. A large amount of radioactivity was present in the triglyceride fraction from patients with pancreatic insufficiency, while such was found only in non-esterified fatty acid fraction in patients with short bowel syndrome, as determined by thin-layer chromatogram scanning. Thus, the "Digestion-Absorption Index" was calculated as a ratio of the radioactivity of triglyceride to that of non-esterified fatty acid. Mean Digestion-Absorption Index was 0.946 in the maldigestive group, and 0.045 in the malabsorptive group, and there was a statistical significance. However, an overlap in a few cases was confirmed in the area of 0.10 +/- 0.02, when this index was plotted on a table of semilogarithms. We assumed that this overlap was chiefly the result of maldigestion plus malasorption of fat.

Topics: Adult; Aged; Autoradiography; Chromatography, Thin Layer; Diagnosis, Differential; Dietary Fats; Digestion; Digestive System Diseases; Fatty Acids, Nonesterified; Feces; Humans; Intestinal Absorption; Iodine Radioisotopes; Lipid Metabolism; Malabsorption Syndromes; Middle Aged; Triglycerides; Triolein

To determine whether or not purified 131I-triolein produces the same behavior as 14C-triolein in the intraluminal phase, and to assess whether or not 0.10 +/- 0.02 of the Digestion-Absorption Index (DAI) obtained from clinical cases is an adequate value for differentiating maldigestion from malabsorption of fat, dogs, pancreatic juice-deficient, short bowel and bile-deficient were prepared. Both the pancreatic juice-deficient and bile-deficient groups indicated a high fecal excretion of 14C- and 131I-radioactivity, and the short bowel group indicated a moderate fecal excretion of fat. Daily fecal fat levels in each group were in parallel to the 3-day fecal excretion of radioactivity. However, the Digestive-Adsorption Index of the pancreatic juice-deficient group of 0.602 in 14C and 0.620 in 131I, and indicated that a considerable portion of the ingested triolein was excreted into the feces without hydrolysis. DAI in the short bowel group were 0.020 in 14C and 0.022 in 131I, and in the bile-deficient group, the indices were 0.031 in 14C and 0.021 in 131I. Both latter groups showed a malabsorptive defect. Additionally, the Digestion-Absorption Index of the pancreatic juice-deficient group was higher than 0.10 +/- 0.02 of the clinical borderline mentioned in the foregoing paper, and that of the other two groups was lower. Therefore, this borderline may represent adequate values for the differentiation of fat malassimilation. On the other hand, it was confirmed that 14C-triolein and 131I-triolein showed similar behavior in the intraluminal phase, and that the 131I-triolein test was adequate for clinical testing for fat digestion and absorption.

Topics: Animals; Bile; Carbon Radioisotopes; Common Bile Duct; Diagnosis, Differential; Digestion; Digestive System Diseases; Dogs; Feces; Intestinal Absorption; Iodine Radioisotopes; Ligation; Pancreas; Pancreatic Juice; Triolein