triolein and Biliary-Fistula

The simultaneous use of 131I-triolein and 75Se-triether, a nonabsorbable marker, in a single oral dose to estimate fat absorption from incomplete fecal collections was tested in rats with and without induced steatorrhea. The results in normal rats showed that analysis of single stool samples allows a valid estimate of fat absorption as defined by the balance study based on the total fecal recovery of 131I. However, in the few normal rats with poor absorption, the absorption values from successively excreted stools showed a tendency to increase. Similar findings were obtained from biliary fistula rats with marked steatorrhea. No evidence of different intestinal transit rates of test fat and marker was observed in bile-diverted rats, thus suggesting that the observed inconsistency in fat absorption values from different stool specimens reflects intraprandial differences in the absorption of fat. Studies on rats subjected to intestinal ischemia do not support the suggestion of others that separation of fat and triether will occur if a mucosal defect is involved as the cause of fat malabsorption. It is concluded that this dual isotope technique may be of value in the clinical estimation of fat absorption, as it offers important technical advantages over conventional fat balance studies.

Topics: Animals; Biliary Fistula; Ethers; Feces; Intestinal Absorption; Intestines; Iodine Radioisotopes; Ischemia; Lipid Metabolism; Radioisotopes; Rats; Selenium; Triolein

Topics: Animals; Bile; Bile Acids and Salts; Biliary Fistula; Cholesterol; Diet; Dietary Fats; Fasting; Fatty Acids, Essential; Female; Haplorhini; Intestinal Fistula; Linoleic Acids; Lipid Metabolism; Macaca; Male; Phospholipids; Secretory Rate; Triglycerides; Triolein

The microsomal fraction and the 18,000 g supernatant fluid obtained from livers from normal rats, cholestyraminetreated rats, or from rats with a bile fistula have been used to compare the 7alpha-hydroxylation of [4-(14)C]cholesterol and beta-[4-(14)C]sitosterol (24alpha-ethyl-cholesterol). It was not possible to increase the specific formation of 7alpha-hydroxy-beta-sitosterol above 0.05% with any of the preparations. This conversion was less than 1% of that found for cholesterol. The inhibitory effect of added 7-oxo- and 7beta-hydroxy-beta-sitosterol on the 7alpha-hydroxylation of cholesterol was found to be much less than that of the corresponding cholesterol compounds. 7alpha-Hydroxy-beta-sitosterol was without effect. It is concluded that the activity of the cholesterol 7alpha-hydroxylase is dependent upon the structure of the steroid side chain.

Topics: Animals; Biliary Fistula; Carbon Isotopes; Cell-Free System; Cholesterol; Cholestyramine Resin; Chromatography, Gas; Chromatography, Ion Exchange; Chromatography, Thin Layer; Circadian Rhythm; Hydroxylation; Liver; Male; Mass Spectrometry; Microsomes, Liver; Phenobarbital; Phytosterols; Rats; Sitosterols; Steroid Hydroxylases; Time Factors; Triolein

Topics: Animals; Bile Acids and Salts; Biliary Fistula; Carbon Isotopes; Duodenum; Intestinal Absorption; Intestinal Mucosa; Jejunum; Lymph; Male; Oleic Acids; Palmitic Acids; Rats; Solubility; Stearic Acids; Time Factors; Triglycerides; Triolein; Tritium

Topics: Animals; Biliary Fistula; Fatty Acids; Glycerides; Intestinal Absorption; Intestinal Mucosa; Rats; Triolein

Topics: Absorption; Animals; Biliary Fistula; Biological Transport; Carbon Isotopes; Fistula; Intestinal Absorption; Lymph; Oleic Acids; Radiometry; Rats; Thoracic Duct; Triolein