tribulus and Disease-Models--Animal

Tribulus terrestris saponins (TTS) have been longley used as an overall tonic and recent studies showed they influence inflammatory conditions. We examined the ameliorative effect of a commercial formula of a saponin-rich extract of TT in a model of dietary obesity in female rats focusing on their ability to control the inflammatory burden, insulin resistance (IR), adipokine expression and the related reproductive system pathologies. Female rats were fed with high fat diet (HFD) for 14 weeks to launch diet-induced obesity; they were assigned as: the obese control female rats (OFR) which received no treatment and TTS (5 and 10 mg/kg/day) treated rats; they were compared to a normal rat group. We determined the IR index, serum/tissue inflammatory cytokines, and adipose tissue adipokine expression and examined the secondary ovarian pathologies. Body weight gain, serum triglycerides and IR (>5-fold) in the OFR group were greater than the normal group; TTS lessened these parameters compared with the OFR group. TTS, at 10 mg/kg dose, ameliorated mRNA expression of leptin and visfatin genes in addition to serum inflammatory cytokine levels. Moreover, TTS corrected the hyperprolactinemia and other hormonal disturbances and ameliorated the ovarian pathologies. This study highlighted that the anti-inflammatory properties of TTS helped in alleviation of IR and body weight gain in OFR. Upon correction of obesity manifestations, the gonadal hormone dysregulations and ovarian pathologies were subsequently ameliorated. We can consider TTS as a promising candidate that may alleviate the inflammatory burden, IR and adipokine expression in obesity and hence prevent the secondary gonadal complications in female subjects if appropriate clinical studies are available.

Topics: Adipokines; Animals; Body Weight; Cytokines; Diet, High-Fat; Disease Models, Animal; Female; Gonadal Disorders; Hyperprolactinemia; Inflammation Mediators; Insulin Resistance; Obesity; Plant Extracts; Rats; Rats, Wistar; Saponins; Tribulus; Triglycerides; Weight Gain

Tribulus terrestris L., as an annual herb plant from Zygophyllaceae, exhibits many biological activities, and its main chemical constituents are saponins. However, the extraction process, chemical compositions, anti-inflammatory effect and mechanism of total saponins from Tribulus terrestris L. leaves are still unclear.. The present study extensively evaluated the extraction process, major components, anti-inflammatory action and mechanism of Tribulus terrestris L. leaves saponins.. The ultrasonic extraction and response surface methods were adopted for optimization of extraction technology of total saponins from Tribulus terrestris L. leaves, and its compositions were detected with LC-MS. The ultrasonic extraction parameters of saponins fraction, including ethanol concentration 30%, extraction time 55 min, ratio of solvent to material 35:1 ml/g and extraction temperature 46 °C, were screened by response surface method with the extracting rate 5.49%, and thirty compositions were detected with LC-MS. These studies imply that Tribulus terrestris L. leaves saponins may be an important anti-inflammatory drug in clinic.

Topics: Acute Lung Injury; Animals; Anti-Inflammatory Agents; Chromatography, High Pressure Liquid; Disease Models, Animal; Lipopolysaccharides; Male; Mice; Plant Extracts; Plant Leaves; RAW 264.7 Cells; Saponins; Spectrometry, Mass, Electrospray Ionization; Tribulus; Ultrasonics

Topics: Animals; Antioxidants; Biomarkers; Biopsy; Body Weight; Disease Models, Animal; Drug Evaluation, Preclinical; Female; Kidney Calculi; Male; p38 Mitogen-Activated Protein Kinases; Plant Extracts; Rats; Tribulus; Urolithiasis

Stroke is one of the most common neurological disorders and seriously threatens human life. Gross saponins of

Topics: Animals; Disease Models, Animal; Gas Chromatography-Mass Spectrometry; Gene Expression Regulation; Male; Metabolic Networks and Pathways; Metabolome; Metabolomics; Neuroprotective Agents; Rats; Saponins; Stroke; Tetrazolium Salts; Tribulus

Topics: Acetylcholinesterase; Administration, Oral; Aluminum Chloride; Alzheimer Disease; Animals; Antioxidants; Behavior, Animal; Brain; Disease Models, Animal; Female; Male; Neuroprotective Agents; Plant Extracts; Plants, Medicinal; Rats, Wistar; Toxicity Tests, Acute; Tribulus

Hyperurecemia is usually associated with gout and various metabolic arthritis disorders. Limited medications are available to manage such conditions. This study aimed to isolate the triterpenes constituent of the plant and to assess xanthine oxidase (XO) inhibitory and antihyperuricemic activities of Tribulus arabicus ethanolic extract, its fractions and the isolated compound using in vitro and in vivo approaches.. The ethanolic extract, fractions; n-hexane, chloroform and n-butanol and the isolated compound (ursolic acid) were evaluated in vitro for their XO inhibitory activity. Those that demonstrated significant activity were further evaluated for their antihyperuricemic activity on potassium oxonate-induced hyperuricemia in mice.. The ethanolic extract was found to be safe up to 5000 mg/kg. The extract and its n-hexane fraction exhibited significant inhibitory activity on XO, whilst only a modest reduction in the enzymatic activity was noticed with n-butanol and chloroform fractions. Furthermore, administration of the ethanolic extract at low and high doses significantly reduced serum urate levels in mice by 31.1 and 64.6% respectively. The isolated active constituent, ursolic acid, showed potent XO inhibition activity (Half maximal inhibitory concentration, IC50 = 10.3 μg/mL), and significantly reduced uric acid level in vivo by 79.9%. Virtually, the binding mode of ursolic acid with XO was determined using molecular docking simulations.. The activity of the ethanolic extract of T. arabicus and its n-hexane fraction can be attributed to the isolated compound, ursolic acid. Ursolic acid has good hypouricemic activity and therefore has high potential to be used for the treatment of gout and hyperuricemia-related diseases.

Topics: Animals; Anti-Infective Agents; Disease Models, Animal; Gout; Humans; Hyperuricemia; Mice; Molecular Docking Simulation; Plant Extracts; Tribulus; Triterpenes; Ursolic Acid; Xanthine Oxidase

Strong evidence proposes that brain oxidative DNA damage and microglia activation contribute to Parkinson's disease (PD) pathogenesis. Traditional therapeutic regimens for PD can only relieve the symptoms. Tribulus terrestris (T. terrestris), a flowering plant from family Zygophyllaceae, is used in traditional medicine for treating different disorders and exerts neuroprotective and antioxidant effects in experimental models. The current study attempted to test whether treatment with T. terrestris standardized extract (TTE) can improve motor dysfunction and alleviate rotenone induced oxidative DNA damage and neurotoxicity in mice. Six groups of male Swiss albino mice were utilized. Group (1) was the vehicle (oil) group, group 2 was the rotenone control group (1 mg/kg/48 hours, subcutaneously) for 9 times, groups 3 and 4 were injected with rotenone and treated with TTE (5 or 10 mg per kg, by oral gavage) for 17 days, groups 5 and 6 served as TTE (5 or 10 mg per kg) per se groups. Motor function was measured by the pole and the open-field tests. Then, mouse brains were dissected, one hemisphere was employed for biochemical assays and the other one was used in histopathological studies. Results demonstrated that TTE ameliorated the motor dysfunctions induced by rotenone as well as markers of inflammation and DNA damage (8-OHdG and MTH1 expression). Indicators of oxidative stress and upregulation of the microglia marker (CD

Topics: Animals; Antioxidants; Disease Models, Animal; DNA Damage; Dopamine; Mice; Microglia; Neuroprotective Agents; Oxidative Stress; Parkinson Disease; Plant Extracts; Rotenone; Substantia Nigra; Tribulus; Up-Regulation

Tribulus terrestris L. (T. terrestris) has been used as a traditional medicine for the treatment of diuretic, lithontriptic, edema and urinary infections. Previous studies have indicated that it is effective in improving inflammation by regulating tumor necrosis factor‑α (TNF)‑α, interleukin (IL)‑6, IL‑10, nitric oxide (NO) and cyclooxygenase (COX)‑2. However, the effects and mechanism of action of T. terrestris on osteoarthritis (OA) remain unknown. Therefore, the present study aimed to evaluate the effects of the ethanolic extract of T. terrestris (ETT) in a monosodium iodoacetate (MIA)‑induced OA animal model. OA was induced in LEW/SSNHSD rats by intra‑articular injection of MIA. Morphometric changes and parameters of the tibial trabecular bone were determined using micro‑computed tomography. The molecular mechanisms of ETT in OA were investigated using reverse transcription‑polymerase chain reaction, western blotting and gelatin zymogram analysis. Treatment with ETT attenuated MIA‑induced OA, and this effect was mediated by the downregulation of NO synthase 2, COX‑2, TNF‑α and IL‑6. Furthermore, the ETT‑mediated attenuation of OA was also dependent on the expression of matrix metalloproteinases‑2 and ‑9. The results of the current study indicate that further evaluation of the mechanisms underlying the attenuation of MIA‑induced OA by ETT are required, and may support the development of ETT as a potential therapeutic agent for the treatment of inflammatory diseases such as OA.

Topics: Animals; Bone and Bones; Cartilage; Cyclooxygenase 2; Cytokines; Disease Models, Animal; Female; Inflammation; Iodoacetic Acid; Matrix Metalloproteinase 2; Matrix Metalloproteinase 9; Nitric Oxide Synthase Type II; Osteoarthritis; Pain; Plant Extracts; Rats; RNA, Messenger; Signal Transduction; Tribulus

Polycystic ovary syndrome (PCOS) is a major hyperandrogenic disorder. Many drugs prescribed specifically to treat PCOS have side effects; however, previous studies suggest that natural therapeutics including botanicals may be less invasive and equally effective for the management of PCOS.. In the present study, plants were screened for antiandrogenic activity using the RIKILT yeast Androgen bioAssay (RAA). Selected positive plants were subsequently tested for their efficacy against PCOS induced by estradiol valerate (EV) in rat models.. RAA revealed the antiandrogenic property of Nardostachys jatamansi DC (NJ), Tribulus terrestris L. (TT), and Embelia tsjeriam-cottam DC (EJ), whereas Whithania somnifera Dunal (WS), Symplocos racemosa Roxb. (SR), and Helicteres isora L. (HI) exhibited androgenic properties. EJ also exhibited mild androgenic activity and therefore was excluded from further study. EV administration reduced the weight gain and disrupted cyclicity in all rats. NJ and TT extract treatment normalized estrous cyclicity and steroidal hormonal levels and regularized ovarian follicular growth.. The in vitro antiandrogenic activity of plant extracts and their positive effects on different parameters of PCOS were proved in vivo.

Topics: Androgen Antagonists; Animals; Biomarkers; Disease Models, Animal; Estradiol; Estrous Cycle; Female; Nardostachys; Ovarian Follicle; Phytotherapy; Plant Extracts; Plants, Medicinal; Polycystic Ovary Syndrome; Progesterone; Rats, Sprague-Dawley; Testosterone; Time Factors; Tribulus; Weight Gain

The aim of this study was to investigate whether terrestrosin D (TED) inhibits the progression of castration-resistant prostate cancer and consider its mechanism.. Cell cycle, mitochondrial membrane potential (ΔΨm) and apoptosis were determined by flow cytometry. Caspase-3 activity and vascular endothelial growth factor secretion were detected by a caspase-3 assay and human vascular endothelial growth factor kit, respectively. A PC-3 xenograft mouse model was used to evaluate the anticancer effect of TED in vivo.. In vitro, TED strongly suppressed the growth of prostate cancer cells and endothelial cells in a dose-dependent manner. TED induced cell cycle arrest and apoptosis in PC-3 cells and human umbilical vascular endothelial cells (HUVECs). TED-induced apoptosis did not involve the caspase pathway. TED also decreased ΔΨm in PC-3 cells and HUVECs. In vivo, TED significantly suppressed tumor growth in nude mice bearing PC-3 cells, without any overt toxicity. Immunohistochemical analysis showed TED induced apoptotic cell death and inhibited angiogenesis in xenograft tumor cells.. Cell cycle arrest and induction of apoptosis in cancer cells and endothelial cells might be plausible mechanisms of actions for the observed antitumor and antiangiogenic activities of TED.

Topics: Adenocarcinoma; Animals; Apoptosis; Caspase 3; Cell Cycle; Cell Line, Tumor; Cell Proliferation; Disease Models, Animal; Endothelium, Vascular; Heterografts; Humans; In Vitro Techniques; Male; Mice; Mice, Nude; Neovascularization, Pathologic; Plant Extracts; Prostatic Neoplasms; Saponins; Tribulus; Vascular Endothelial Growth Factor A; Xenograft Model Antitumor Assays

Tribulus terrestris has long been used in traditional medicine to treat impotency and improve sexual functions in man. The aim of this study was to evaluate the efficiency of T. terrestris extract in the treatment of polycystic ovary (PCO) in Wistar rat. Estradiol valerate was injected to 15 mature Wistar rats to induce PCO. Rats were randomly divided into three groups (control, low-dose and high-dose groups) of five each and received 0, 5 and 10 mg of T. terrestris extract, respectively.Treatments began on days 50 and 61 after estradiol injection; at the same time, vaginal smear was prepared. The ovaries were removed on day 62, and histological sections were prepared accordingly. The number and diameter of corpora lutea, thickness of the theca interna layer and the number of all follicles were evaluated in both ovaries. In comparison with the control group, the number of corpora lutea and primary and secondary follicles significantly increased following T. terrestris treatment; however, the number of ovarian cysts significantly decreased. It can be concluded that T. terrestris have a luteinizing effect on ovarian cysts, which may relate to its gonadotropin-like activity; also, a high dose of the extract can efficiently remove ovarian cysts and resume ovarian activity.

Topics: Animals; Corpus Luteum; Disease Models, Animal; Estradiol; Female; Ovarian Cysts; Ovarian Follicle; Phytotherapy; Plant Extracts; Rats; Rats, Wistar; Tribulus; Vaginal Smears

Phytotherapy has played an important role in the management of diabetes and related complications. In the present study different fractions of Catharanthus roseus L. (Apocynaceae), Ocimum sanctum L. (Labiatae), Tinospora cordifolia Willd. (Menispermaceae), Aegle marmelos L. (Rutaceae), Ficus golmerata L. (Moraceae), Psoralea corlifolia L. (Fabaceae), Tribulus terrestris L. (Zygophyllaceae), and Morinda cetrifolia L. (Rubiaceae) were evaluated as possible inhibitors of aldose reductase (AR: a key enzyme implicated in cataractogenesis) and antioxidant agents. Anti-cataract activity of the selected plants was demonstrated using 'sugar induced lens opacity model' and the cytotoxicity studies were carried out using MTT assay. Among the tested plants, water extract of M. cetrifolia (IC50 0.132 mg/ml) exhibited maximum AR inhibitory activity as compared to other phytofractions which showed the activity in an IC50 range of 0.176-0.0.82 mg/ml. All the plant fractions showed considerable antioxidant potential. Sugar induced lens opacity studies revealed that, M. cetrifolia possess significant anti-cataract potential to maintain lens opacity as compared to glucose induced lens opacity in bovine lens model. The extract of the selected plants showed moderate cytotoxicity against HeLa cell line. Results of the present studies may find useful in converting botanicals into therapeutic modalities.

Topics: Aegle; Aldehyde Reductase; Animals; Ascorbic Acid; Cataract; Catharanthus; Cattle; Diabetes Complications; Disease Models, Animal; Enzyme Inhibitors; Ficus; Flavonoids; Free Radical Scavengers; HeLa Cells; Humans; Hypoglycemic Agents; Male; Menispermaceae; Morinda; Ocimum; Phenols; Phytotherapy; Plant Extracts; Plants, Medicinal; Psoralea; Rats; Tinospora; Tribulus

Tribulosin (tigogenin 3-O-β-D-xylopyranosyl(1-2)-[β-D-xylopyranosyl (1-3)]-β-D-glucopyranosyl (1-4)-[a-L-rhamnopyranosyl(1-2)]-β-D-galactopyranoside), a component of gross saponins of Tribulus terrestris, has been shown to produce cytoprotective effects in heart. Yet, the precise mechanisms are not fully understood. We examined the mechanisms of tribulosin on myocardial protection. Ventricular myocytes were isolated from the heart of neonatal rats and were exposed to 3 h of hypoxia followed by 2 h reoxygenation. Apoptosis was induced by hypoxia/reoxygenation (H/R), and the expression of protein kinase C epsilon (PKCϵ) and extracellular signal-regulated kinase 1 and 2 (ERK1/2) in cultured neonatal rat cardiac myocytes was detected. The results indicated that treatment with tribulosin in the culture medium protected cardiac myocytes against apoptosis induced by H/R. PKCϵ and ERK1/2 expression increased after pretreated with tribulosin. In the presence of PKCϵ inhibitor co-treated with tribulosin, the expression of ERK1/2 was decreased in H/R cardiac myocytes. While preconditioned with PD98059, ERK1/2 inhibitor, no effects on the expression of PKCϵ were detected. Tribulosin has protective effects on cardiac myocytes against apoptosis induced by H/R injury via PKCϵ and ERK1/2 signaling pathway.

Topics: Animals; Animals, Newborn; Apoptosis; Disease Models, Animal; Hypoxia; Male; Mitogen-Activated Protein Kinase 3; Myocytes, Cardiac; Protein Kinase C-epsilon; Rats; Rats, Wistar; Sitosterols; Tribulus

Hormonal effects of Tribulus terrestris (TT) were evaluated in primates, rabbit and rat to identify its usefulness in the management of erectile dysfunction (ED). TT extract was administered intravenously, as a bolus dose of 7.5, 15 and 30 mg/kg, in primates for acute study. Rabbits and normal rats were treated with 2.5, 5 and 10mg/kg of TT extract orally for 8 weeks, for chronic study. In addition, castrated rats were treated either with testosterone cypionate (10mg/kg, subcutaneously; biweekly for 8 weeks) or TT orally (5mg/kg daily for 8 weeks). Blood samples were analyzed for testosterone (T), dihydrotestosterone (DHT) and dehydroepiandrosterone sulphate (DHEAS) levels using radioimmunoassay. In primates, the increases in T (52%), DHT (31%) and DHEAS (29%) at 7.5mg/kg were statistically significant. In rabbits, both T and DHT were increased compared to control, however, only the increases in DHT (by 30% and 32% at 5 and 10mg/kg) were statistically significant. In castrated rats, increases in T levels by 51% and 25% were observed with T and TT extract respectively that were statistically significant. TT increases some of the sex hormones, possibly due to the presence of protodioscin in the extract. TT may be useful in mild to moderate cases of ED.

Topics: Administration, Oral; Animals; Blood Pressure; Castration; Dehydroepiandrosterone Sulfate; Dihydrotestosterone; Disease Models, Animal; Dose-Response Relationship, Drug; Electrocardiography; Erectile Dysfunction; Injections, Subcutaneous; Macaca mulatta; Male; Papio anubis; Phytotherapy; Plant Extracts; Rabbits; Rats; Rats, Sprague-Dawley; Species Specificity; Testosterone; Tribulus

This experiment was designed to determine whether Tribuli saponins (TS) relieve left ventricular remodeling (VR) after myocardial infarction (MI) in a murine hyperlipemia (HL) model. MI and HL models were induced and high and low doses of TS and simvastatin were administrated to the rats. Four weeks later, echocardiographic observation was performed and the left and right ventricular weight index (LVWI, RVWI) was calculated. Echocardiographic results showed that both high dose of TS and simvastatin had a beneficial effect on increasing fractional shortening (FS) and ejection fraction (EF), reducing left ventricular end diastolic volume (LVEDV), systolic volume (LVESV), left ventricular dimension end diastole (LVDd) and systole (LVDs), and decreasing LVWI, as compared to those in the HL-MI model group (p < 0.05, 0.01). Both medicines had little impact on thickness of the anterior and posterior wall. No significant difference was observed between each treatment group (p > 0.05). In conclusion, TS not only lowered serum lipidemia, but also relieved left ventricular remodeling, and improved cardiac function in the early stage after MI.

Topics: Animals; Cardiac Volume; Cholesterol; Disease Models, Animal; Dose-Response Relationship, Drug; Heart Ventricles; Hydroxymethylglutaryl-CoA Reductase Inhibitors; Hyperlipidemias; Male; Myocardial Infarction; Plant Extracts; Random Allocation; Rats; Rats, Sprague-Dawley; Saponins; Simvastatin; Stroke Volume; Tribulus; Ultrasonography; Ventricular Remodeling

Antifungal activity of natural products is being studied widely. Saponins are known to be antifungal and antibacterial. We have isolated eight steroid saponins from Tribulus terrestris L., namely TTS-8, TTS-9, TTS-10, TTS-11, TTS-12, TTS-13, TTS-14 and TTS-15. TTS-12 and TTS-15 were identified as tigogenin-3-O-beta-D-xylopyranosyl(1-->2)-[beta-D-xylopyranosyl(1-->3)]-beta-D-glucopyranosyl(1-->4)-[alpha-L-rhamnopyranosyl(1-->2)]-beta-D-galactopyranoside and tigogenin-3-O-beta-D-glucopyranosyl(1-->2)-[beta-D-xylopyranosyl(1-->3)]-beta-D-glucopyranosyl(1-->4)-beta-D-galactopyranoside, respectively. The in vitro antifungal activities of the eight saponins against six fluconazole-resistant yeasts, Candida albicans, Candida glabrata, Candida parapsilosis, Candida tropicalis, Candida krusei, and Cryptococcus neoformans were studied using microbroth dilution assay. The results showed that TTS-12 and TTS-15 were very effective against several pathogenic candidal species and C. neoformans in vitro. It is noteworthy that TTS-12 and TTS-15 were very active against fluconazole-resistant C. albicans (MIC(80)=4.4, 9.4 microg/ml), C. neoformans (MIC(80)=10.7, 18.7 microg/ml) and inherently resistant C. krusei (MIC(80)=8.8, 18.4 microg/ml). So in vivo activity of TTS-12 in a vaginal infection model with fluconazole-resistant C. albicans was studied in particular. Our studies revealed TTS-12 also showed in vivo activities against fluconazole-resistant yeasts. In conclusion, steroid saponins TTS-12 and TTS-15 from Tribulus terrestris L. have significant in vitro antifungal activity against fluconazole-resistant fungi, especially TTS-12 also showed in vivo activity against fluconazole-resistant C. albicans.

Topics: Animals; Antifungal Agents; Candida; Cryptococcus neoformans; Disease Models, Animal; Drug Resistance, Fungal; Drugs, Chinese Herbal; Female; Fluconazole; Galactose; Humans; Microbial Sensitivity Tests; Saponins; Steroids; Time Factors; Tribulus; Vaginal Diseases

FT-IR spectra of liver tissue isolated from mice, Mus musculus, have been recorded in the region of 4000 - 400 cm-1 for normal, mercury treated and recovery phase. In this study, the total protein content was found to be decreased in the liver tissues after treatment with median-lethal dose of mercuric chloride. The marked fall in the level of bio-chemical constituent in the tissue due to metal exposure indicates the rapid initiation of the breakdown of the bio-chemical constituents to meet the energy demand during toxic stress. During the recovery phase, the decreased levels of bio-chemical constituents are restored to near normal level. Methanol fractions of Tribulus terrestris fruit extract was administrated on mercury intoxicated mice for 15 days. After the administration, the mercury-intoxicated animals slowly recovered from the adverse effect of mercury poisoning with the help of plant bio-formulations. The results are discussed in detail.

Topics: Animals; Chemical and Drug Induced Liver Injury; Disease Models, Animal; Fruit; Liver; Mercuric Chloride; Mercury Poisoning; Mice; Phytotherapy; Plant Extracts; Protective Agents; Spectrophotometry, Infrared; Tribulus

Tribulus terrestris is a natural herb used for treating many diseases including hypertension. According to previous reports, aqueous extract of tribulus fruits may have some antihypertensive effect with an unknown mechanism. The present study investigated the antihypertensive mechanism of tribulus in 2K1C hypertensive rats by measurement of circulatory and local ACE activity in aorta, heart, kidney and lung. Four groups of rats were selected; control, sham, operated or hypertensive and tribulus treated hypertensive group. Hypertension was induced using silver clip on renal artery by surgery. Four weeks after surgery, a single daily dose of 10 mg/kg of lyophilized aqueous extract of tribulus fruit were given orally to 2K1C rats for four weeks. ACE activity was determined by high performance liquid chromatography (HPLC). Blood pressure was measured by the tail-cuff method. The systolic blood pressure (SBP) was significantly increased in 2K1C rats compared to control rats. The SBP of tribulus fed hypertensive rats was significantly decreased compared to hypertensive rats. The ACE activity in all tissues of 2K1C rats including: aorta, heart, kidney, lung as well as serum were significantly increased compared to normal rats. The ACE activity in all tissues of tribulus fed hypertensive rats was significantly lower than that of hypertensive rats, which was more pronounced in kidney. These results indicated that there is a negative correlation between consumption of tribulus and ACE activity in serum and different tissues in 2K1C rats.

Topics: Angiotensin-Converting Enzyme Inhibitors; Animals; Antihypertensive Agents; Blood Pressure; Disease Models, Animal; Hypertension, Renovascular; Male; Peptidyl-Dipeptidase A; Phytotherapy; Plant Preparations; Rats; Rats, Wistar; Tribulus