tribulus and Acute-Lung-Injury

Tribulus terrestris L., as an annual herb plant from Zygophyllaceae, exhibits many biological activities, and its main chemical constituents are saponins. However, the extraction process, chemical compositions, anti-inflammatory effect and mechanism of total saponins from Tribulus terrestris L. leaves are still unclear.. The present study extensively evaluated the extraction process, major components, anti-inflammatory action and mechanism of Tribulus terrestris L. leaves saponins.. The ultrasonic extraction and response surface methods were adopted for optimization of extraction technology of total saponins from Tribulus terrestris L. leaves, and its compositions were detected with LC-MS. The ultrasonic extraction parameters of saponins fraction, including ethanol concentration 30%, extraction time 55 min, ratio of solvent to material 35:1 ml/g and extraction temperature 46 °C, were screened by response surface method with the extracting rate 5.49%, and thirty compositions were detected with LC-MS. These studies imply that Tribulus terrestris L. leaves saponins may be an important anti-inflammatory drug in clinic.

Topics: Acute Lung Injury; Animals; Anti-Inflammatory Agents; Chromatography, High Pressure Liquid; Disease Models, Animal; Lipopolysaccharides; Male; Mice; Plant Extracts; Plant Leaves; RAW 264.7 Cells; Saponins; Spectrometry, Mass, Electrospray Ionization; Tribulus; Ultrasonics