tretinoin and Polycystic-Kidney--Autosomal-Dominant

Autosomal dominant polycystic kidney disease (ADPKD) is a genetic disorder characterized by uncontrollable epithelial cell growth, cyst formation, and kidney malfunction. In the present study, we investigated the antiproliferative effects of the treatment with the combination of paclitaxel (PAC) and all-trans retinoic acid (ATRA) on ADPKD epithelial cells. Our results show that the combined treatment with 1 nM PAC and 10 nM ATRA significantly suppressed ADPKD cell proliferation (20%), while the treatment with ATRA or PAC alone had no such effect. Treatment with PAC and ATRA induced cell cycle arrest at the G2/M phase and apoptosis by upregulating p53 and caspase-8 expression and increased the intracellular calcium (Ca

Topics: Apoptosis; Calcium; Caspase 8; Cell Cycle Checkpoints; Cell Line; Cell Proliferation; Drug Synergism; Epithelial Cells; Humans; Intracellular Space; MAP Kinase Signaling System; Models, Biological; Paclitaxel; Polycystic Kidney, Autosomal Dominant; Tretinoin; Tumor Suppressor Protein p53