tretinoin and Peripheral-Nervous-System-Neoplasms

Topics: Cell Transformation, Neoplastic; Gene Expression Regulation; Humans; Neuroblastoma; Peripheral Nervous System Neoplasms; Proto-Oncogene Proteins; Proto-Oncogene Proteins c-myc; Transcription, Genetic; Tretinoin

Neuroblastoma (NB) is an infant tumor which frequently differentiates into neurons. We used two-dimensional differential in-gel electrophoresis (2D-DIGE) to analyze the cytosolic and nuclear protein expression patterns of LAN-5 cells following neuronal differentiating agent all-trans-retinoic acid treatment. We identified several candidate proteins, from which G beta2 and Prefoldin 3 may have a role on NB development. These results strength the use of proteomics to discover new putative protein targets in cancer.

Topics: Cell Line, Tumor; Cell Nucleus; Chromatography, Liquid; Computational Biology; Cytosol; Electrophoresis, Gel, Two-Dimensional; Humans; Neoplasm Proteins; Neuroblastoma; Peripheral Nervous System Neoplasms; Protein Array Analysis; Proteomics; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Tretinoin

Two new cell lines, designated NMS-2 and NMS-2PC, were established in vitro from a malignant peripheral nerve sheath tumour (MPNST) in the right thigh and a retroperitoneal lesion of a 30-year-old man with neurofibromatosis type 1 (NF1). The NMS-2 cell line was derived from the first tumour, and the NMS-2PC cell line from a retroperitoneal metastatic tumour detected 9 months later. Cultured NMS-2 cells showed epithelioid features, while NMS-2PC cells showed fibroblast-like features. However, both cell lines were strongly positive for S-100 protein. The transplanted NMS-2 and NMS-2PC tumours showed the same histological features typical of MPNST. Chromosomal analysis revealed that only the NMS-2 cells had a t (1;2) chromosomal translocation. Chemosensitivity tests demonstrated that NMS-2PC cells were far more sensitive than NMS-2 cells to Adriamycin and etoposide, which had been used clinically. All-trans-retinoic acid induced a morphological change in NMS-2PC cells so that they were no longer fibroblast-like, but epithelioid cells. We believe the epitheloid components in the MPNST were derived from typical spindle cells.

Topics: Adult; Animals; DNA Primers; Doxorubicin; Drug Screening Assays, Antitumor; Etoposide; Female; Humans; Immunoenzyme Techniques; Karyotyping; Male; Mice; Mice, Inbred BALB C; Mice, Nude; Neoplasm Transplantation; Neurofibromatosis 1; Peripheral Nervous System Neoplasms; Polymerase Chain Reaction; Retroperitoneal Neoplasms; S100 Proteins; Translocation, Genetic; Tretinoin; Tumor Cells, Cultured