tretinoin and Laryngeal-Neoplasms

Topics: Anticarcinogenic Agents; Combined Modality Therapy; Head and Neck Neoplasms; Humans; Incidence; Laryngeal Neoplasms; Larynx; Precancerous Conditions; Tretinoin; United States

miR-27a regulates cell differentiation in a variety of diseases. However, whether and how miR-27a participates in laryngeal cancer cell differentiation remains unknown. Therefore, we explored role and molecular mechanism of miR-27a in laryngeal cancer differentiation in the study. We found that miR-27a expression was inversely correlated with laryngeal cancer differentiation degree based on the clinical pathological diagnosis of each patient. miR-27 asignificantly rescued differentiation and inhibited β-catenin, LEF1, OCT4 and SOX2 in Wnt/β-catenin pathway in all-trans-retinoic acid (ATRA)-induced laryngeal cancer cells. Bindings of RARα to miR-27a and miR-27a to GSK-3β were confirmed by ChIP and Luciferase reporter assays, respectively. In conclusion, miR-27a is a negative regulator in laryngeal cancer differentiation. RARα-mediated miR-27a transcriptional inactivation releases the inhibition of miR-27a on GSK-3β leading to laryngeal cancer differentiation through GSK-3β-involved Wnt/β-catenin pathway, suggesting that miR-27a is a usefully therapeutic target at least in ATRA-induced laryngeal cancer differentiation.

Topics: Antineoplastic Agents; beta Catenin; Blotting, Western; Cell Differentiation; Cell Line, Tumor; Cell Survival; Female; Gene Expression Regulation, Neoplastic; Glycogen Synthase Kinase 3 beta; HEK293 Cells; Humans; Laryngeal Neoplasms; Lymphoid Enhancer-Binding Factor 1; Male; MicroRNAs; Middle Aged; Octamer Transcription Factor-3; Retinoic Acid Receptor alpha; Reverse Transcriptase Polymerase Chain Reaction; SOXB1 Transcription Factors; Tretinoin; Wnt Signaling Pathway

Topics: Acne Vulgaris; Carcinoma, Squamous Cell; Diagnosis, Differential; Follow-Up Studies; Humans; Laryngeal Neoplasms; Male; Middle Aged; Neck; Radiation Dosage; Radiodermatitis; Radiotherapy; Risk Assessment; Severity of Illness Index; Tretinoin

To investigate the treatment efficient of viaminati on the laryngeal keratosis.. All 16 cases of laryngeal keratosis took viaminati.. 15 cases were recovered. 1 case was developed cancer.. Viaminati is effective in treating laryngeal keratosis and has not obvious side-effect.

Topics: Adult; Female; Humans; Keratolytic Agents; Keratosis; Laryngeal Diseases; Laryngeal Neoplasms; Male; Middle Aged; Precancerous Conditions; Tretinoin

In vitro monolayer culture and clonogenic assay were used to investigate the individual and combined effect of temperature and retinoic acid (RA) on cellular morphology and colony forming ability of human epidermoid laryngeal carcinoma (HEp-2) cells. 20 micromol. RA alone inhibited multilayer formation and induced cell flattening. Hyperthermia (42 degrees C) individually caused formation of cytoplasmic processes and irregularities in cellular shape and size. Combined effect of hyperthermia (42 degrees C) and 20 micromol. RA treatment caused bleb formation on cell surfaces and lysis of cytoplasmic and nuclear membrane. RA treatment also caused dose-dependent reduction of colony growth. Heat-induced cell killing was only observed at lethal temperatures of 43 degrees C and above. RA in combination with heat synergistically inhibited colony formation even at non lethal temperatures of 41 and 42 degrees C. These results indicate that RA in combination with hyperthermia may facilitate the therapy of human epidermoid larynx carcinoma.

Topics: Antineoplastic Agents; Carcinoma, Squamous Cell; Cell Division; Cell Survival; Combined Modality Therapy; Dose-Response Relationship, Drug; Hot Temperature; Humans; Laryngeal Neoplasms; Tretinoin; Tumor Cells, Cultured

To investigate whether all-trans retinoic acid (RA) has antitumor effect in vivo.. Sixteen nude mice were transplanted with human laryngeal cell carcinoma PHC3. Eight of them were injected with RA intraperitoneally and others with solvent as the control. Response was measured and tumor histopathological features were studied.. The growth and weight of RA-treated tumors were significantly reduced in comparison with those of the control. The growth inhibition rate reached more than 50% in the RA group. Studies also showed that RA-treated cells became well-differentiated. There was a significant decrease of c-myc protein level in the RA treated tumors as compared with the control.. It is suggested that RA could induce differentiation of human laryngeal carcinoma cells and suppress tumor growth in vivo. Regulation of c-myc gene or protein may be related to those happening.

Topics: Animals; Antineoplastic Agents; Female; Humans; Laryngeal Neoplasms; Mice; Mice, Inbred BALB C; Mice, Nude; Neoplasm Transplantation; Proto-Oncogene Proteins c-myc; Tretinoin

To observe the differentiation-inducing effect of Stepholidine (ST) and Retinoic Acid (RA) on laryngeal and nasopharyngeal carcinoma, the expression of oncogene (C-myc, bcl-2) protein and tumor suppressor gene (p53) was done by using flow cytometry and ABC immunohistochemical methods combined with image analysis technique. The results indicated that after treated with ST and RA, the cells became well-differentiated, the cell growth was suppressed, contact suppress was partially recovered, colony forming was decreased, protooncogenes (C-myc bcl-2) protein was decreased, tumor suppressor gene (p53) protein was increased. No significant difference was observed between the cells treated with ST and TA. We believed that the Chinese medicine-Stephania might be expected to become a new prospective differentiation inducer in carcinoma of head and neck.

Topics: Berberine; Cell Differentiation; Cell Division; Humans; Laryngeal Neoplasms; Nasopharyngeal Neoplasms; Proto-Oncogene Proteins c-bcl-2; Tretinoin; Tumor Cells, Cultured; Tumor Suppressor Protein p53

We have defined conditions permitting the in vitro growth of human laryngeal papilloma cells at the air-liquid interface. Using this model system, retinoic acid has been found to modulate the differentiation of human laryngeal papilloma cells along two different pathways. At low concentrations of retinoic acid [10(-9) mol/L and 10(-8) mol/L], the cells formed a stratified squamous epithelium with a differentiation-specific protein staining pattern identical to that found in vivo. At higher concentrations of retinoic acid [10(-7) mol/L and 10(-6) mol/L], the cells differentiated into a columnar epithelium with occasional ciliated cells, lacking the markers of squamous differentiation. Analysis of the human papillomavirus DNA content revealed that as the concentration of retinoic acid increased, the viral DNA content decreased. This system is proposed as a model to further investigate the differentiation defects of human laryngeal papilloma cells and the regulatory role of retinoic acid in the clinical expression of human laryngeal papillomatosis.

Topics: DNA, Viral; Dose-Response Relationship, Drug; Epithelial Cells; Humans; In Vitro Techniques; Laryngeal Neoplasms; Papilloma; Papillomaviridae; Tretinoin; Tumor Cells, Cultured

Laryngeal papillomatosis often involves a relentless growth of papillomas on the vocal cords, requiring repeated excisions to maintain an adequate airway. Because of its antiproliferative effects on epithelial tissues, 13-cis-retinoic acid (0.5-2.0 mg/kd/day p.o.) was used in five patients whose disease was poorly controlled by laser beam surgery. Control of disease for 24+, 5+, and 12 months has been achieved in three of the patients, with two complete and one partial responses. Side effects of treatment were mild and rapidly reversible, following a 25-50% reduction in drug dose.

Topics: Adult; Aged; Antineoplastic Agents; Child; Child, Preschool; Combined Modality Therapy; Drug Evaluation; Female; Humans; Isotretinoin; Laryngeal Neoplasms; Laser Therapy; Male; Middle Aged; Papilloma; Tretinoin

After in vitro incubation of human squamous cell carcinomas of the head and neck region with aromatic retinoid an increased number of lysosomes can be observed in the tumor cells. It is discussed whether this accumulaion of lysosomes is due to a direct stimulation of lysosomal enzyme synthesis or whether it is consequence of cell damage by the retinoid.

Topics: Acid Phosphatase; Acitretin; Carcinoma, Squamous Cell; Culture Techniques; Dose-Response Relationship, Drug; Humans; Laryngeal Neoplasms; Lysosomes; Mouth Neoplasms; Tretinoin