tretinoin and Dry-Eye-Syndromes

Topics: Autophagy; Dry Eye Syndromes; Epithelial Cells; Humans; Inflammation; RNA; Trehalose; Tretinoin

Topics: Animals; Chemokine CCL5; Conjunctiva; Cornea; Dry Eye Syndromes; Eye; Female; Goblet Cells; Homeostasis; Immunity, Innate; Inflammation; Interleukin-12 Subunit p35; Interleukin-1beta; Lacrimal Apparatus; Mice; Mice, Inbred C57BL; Myeloid Cells; Receptors, Retinoic Acid; Retinoids; Signal Transduction; Tretinoin; Vitamin A; Vitamin A Deficiency

Topical retinoic acid has proved to be of variable benefit in a number of dry eye disorders of disparate aetiology, in which squamous metaplasia with keratinization of ocular epithelium is present. Its exact role in patients with dry eye however remains in dispute. We describe a case of severe dry eye due to chronic graft-versus-host disease, which was refractory to conventional therapy but which responded remarkably to topical retinoic acid with reversal of conjunctival keratinization and marked resolution of symptoms.

Topics: Administration, Topical; Bone Marrow Transplantation; Chronic Disease; Dry Eye Syndromes; Graft vs Host Disease; Humans; Male; Middle Aged; Tretinoin

We studied the ultrastructural changes of the corneal epithelium of the experimental rabbit dry eye and the effects of three topically applied eye-drops. Fourty rabbits were divided into experimental rabbit dry eye group, 0.1% bromhexine-treated group, 50% Chinese medicinal herb-treated group and 0.01% retinoic acid-treated group. Ten days after surgical removal of lacrimal gland, nictitating membrane and Harder's gland of the rabbit eyes, and corroding the conjunctiva with 50% trichloroacetic acid, the rabbit eyes showed typical dry eye signs. A lot of pathologic ultrastructural changes of the corneal epithelium were observed. Fourteen days after topically applying the three eye-drops, no significant effect was found on the ultrastructural changes of the dry eye corneal epithelium in the 0.1% bromhexine-treated and 50% Chinese medicinal herb-treated groups. However, in the 0.01% retinoic acid-treated eyes, normal microvilli and microplicae reappeared, denuded corneal epithelium was covered by new epithelial cells, no cell swelled, degenerated mitochondria recovered, basement membrane returned completely, and the completely recovered corneal epithelial cell count showed a significant difference compared with the control (P < 0.01). We conclude that removing the lacrimal gland, nictitating membrane and Harder's gland of rabbit eye, and corroding the conjunctiva with 50% trichloroacetic acid can result in rabbit dry eye and topically applying 0.01% retinoic acid can promote the recovery of the dry corneal epithelium.

Topics: Animals; Bromhexine; Cornea; Drugs, Chinese Herbal; Dry Eye Syndromes; Epithelium; Lacrimal Apparatus; Microscopy, Electron; Ophthalmic Solutions; Rabbits; Tretinoin

Topics: Adult; Conjunctiva; Dry Eye Syndromes; Forecasting; Humans; Keratins; Male; Ointments; Tretinoin

Topics: Dry Eye Syndromes; Ethics; Humans; Ointments; Pharmaceutical Services; Tretinoin

Topics: Aged; Calcinosis; Corneal Diseases; Dry Eye Syndromes; Female; Humans; Male; Tretinoin