tretinoin and Burns--Chemical

Disorders of the transparent cornea affect millions of people worldwide. However, how to maintain and/or regenerate this organ remains unclear. Here, we show that

Topics: Age Factors; Aldehyde Dehydrogenase 1 Family; Animals; Burns, Chemical; Cell Differentiation; Cell Proliferation; Cells, Cultured; Cellular Senescence; Corneal Neovascularization; Corneal Stroma; Disease Models, Animal; Epithelium, Corneal; Eye Burns; Mice, Knockout; Regeneration; Retinal Dehydrogenase; Signal Transduction; Stem Cells; Transcription Factor RelA; Tretinoin

Alkali burns are the most common, severe chemical ocular injuries, their functional prognosis depending on corneal wound healing efficiency. The purpose of our study was to compare the benefits of amniotic membrane (AM) grafts and homogenates for wound healing in the presence or absence of previous all-trans retinoic acid (atRA) treatment.. Fifty male CD1 mice with reproducible corneal chemical burn were divided into five groups, as follows: group 1 was treated with saline solution; groups 2 and 3 received untreated AM grafts or grafts treated with atRA, respectively; and groups 4 and 5 received untreated AM homogenates or homogenates treated with atRA, respectively. After 7 days of treatment, ulcer area and depth were measured, and vascular endothelial growth factor (VEGF) and matrix metalloproteinase 9 (MMP-9) were quantified.. AM induction by atRA was confirmed via quantification of retinoic acid receptor β (RARβ), a well-established retinoic acid-induced gene. Significant improvements of corneal wound healing in terms of ulcer area and depth were obtained with both strategies. No major differences were found between the efficiency of AM homogenates and grafts. This positive action was increased when AM was pretreated with atRA. Furthermore, AM induced a decrease in VEGF and MMP-9 levels during the wound healing process. The atRA treatment led to an even greater decrease in the expression of both proteins.. Amnion homogenate is as effective as AM grafts in promoting corneal wound healing in a mouse model. A higher positive effect was obtained with atRA treatment.

Topics: Alkalies; Amnion; Animals; Burns, Chemical; Corneal Ulcer; Disease Models, Animal; Eye Burns; Fluorescent Antibody Technique, Indirect; Humans; Keratolytic Agents; Male; Matrix Metalloproteinase 9; Mice; Tissue Engineering; Transplants; Tretinoin; Vascular Endothelial Growth Factor A; Wound Healing

An experimental study was carried out to investigate the efficacy of an anti-inflammatory and antiproliferative agent all-trans retinoic acid (ATRA) and an antioxidant agent zinc sulphate (ZnSO(4)) in the prevention of stricture after caustic esophageal burn in rats.. Esophageal burn was induced using 50% NaOH. Rats were divided into four groups as follows: group A (sham; n = 8), group B (control; n = 8), group C (treated with ATRA; n = 8) and group D (treated with ZnSO(4); n = 8). All rats were killed on the 28th day and esophageal tissues were evaluated for histopathologic damage score, hydroxyproline (HP) content and TGF-beta1 expression.. Significant difference was detected in terms of histopathologic damage score between groups B and C (p = 0.002). Although mean HP levels of groups C and D were lower than group B, statistical comparison was not significant. TGF-beta1 expression in group C was significantly lower than group B.. Zinc has not been found effective in the prevention of stricture formation. The results indicate that ATRA has a preventive effect in the development of fibrosis in an experimental model of caustic esophageal burns in rats.

Topics: Animals; Anti-Inflammatory Agents; Antioxidants; Burns, Chemical; Caustics; Disease Models, Animal; Esophageal Stenosis; Esophagus; Oxidative Stress; Rats; Rats, Wistar; Tretinoin; Zinc Sulfate