transforming-growth-factor-beta and Laryngeal-Diseases

The ultrastructural characteristics of new bone and cartilage, induced at the site of cricoid cartilage defects treated with rhBMP-2 in rabbits, were investigated.. A cricoid defect model was used. Fifteen rabbits were randomly and equally divided into 3 groups. Four rabbits from each group were treated with rhBMP-2, while one rabbit from each group was used as control. The rabbits were killed 1, 2, or 4 weeks after surgery. The healing pattern of the laryngeal wound was evaluated by light and transmission electron microscopy.. Mineralized collagen type I matrix, osteoblasts, and osteoclast-like cells were present as early as 1 week after surgery. Well-structured bone trabeculas and growth plate-like structures were present 4 weeks after surgery.. Intramembranous and endochondral osteogenesis take place at the site of cricoid cartilage defects treated with rhBMP-2. Progenitor cells of cricoid perichondrium form a growth plate-like structure similar to the epiphyseal growth plate.. This study reveals the pattern of BMP-2-induced repair of airway cartilage defects.

Topics: Animals; Bone Morphogenetic Protein 2; Bone Morphogenetic Proteins; Cell Differentiation; Cell Proliferation; Chondrocytes; Chondrogenesis; Collagen Type I; Cricoid Cartilage; Disease Models, Animal; Growth Plate; Laryngeal Diseases; Male; Microscopy, Electron, Transmission; Osteoblasts; Osteoclasts; Osteogenesis; Rabbits; Random Allocation; Recombinant Proteins; Time Factors; Transforming Growth Factor beta; Wound Healing

This study seeks to determine the ability of enzyme-linked immunosorbent assays of vocal fold secretions to detect and describe the inflammatory response in the vocal folds. Vocal fold and palatal secretions were collected during operation from patients with a range of vocal fold disorders and from control patients. The secretions were subjected to assays for interleukin-1beta, prostaglandin E2, and transforming growth factor beta. The results indicate a differential expression of mediators associated with the wound healing cascade in the vocal folds. The prostaglandin E2 levels clearly differentiated vocal fold secretions associated with laryngeal disease versus control sites. Furthermore, the interleukin-1beta concentrations were significantly elevated in subjects with epithelial lesions of the vocal folds as opposed to lesions of the lamina propria. Although still in its infancy, such analysis may ultimately hold scientific and clinical utility in the study and management of patients with vocal fold disease.

Topics: Adolescent; Adult; Aged; Biomarkers; Dinoprostone; Humans; Inflammation Mediators; Interleukin-1; Laryngeal Diseases; Middle Aged; Transforming Growth Factor beta; Vocal Cords; Wound Healing